The laccase promoter of potato confers strong tuber-specific expression in transgenic plants

Ha, Jun; Moon, Ki-Beom; Kim, Mi-Sun; Park, Se-Won; Hahn, Kyu-Woong; Jeon, Jun Ho; Kim, Hyun-Soon

doi:10.1007/s11240-014-0578-1

Cited by 6 publications

(2 citation statements)

References 53 publications

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“…The best-known potato promoters are promoters that drive tuber-specific expression. To date, several promoters have been characterized, including promoters of such genes as patatin [ 10 ], granule-bound starch synthase I (GBSSI) [ 11 ], laccase [ 12 ], and glucose pyrophosphorylase [ 13 ]. Both patatin and GBSS promoters have already been successfully used for metabolic engendering in potato to enhance the tuber content of oil [ 14 , 15 ], carotenoid [ 16 ], amylose [ 17 ] and cyanophycin [ 18 ] and for the production of edible vaccines [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Miroshnichenko

Фирсов

Timerbaev

et al. 2020

Plants

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Various plant-derived promoters can be used to regulate ectopic gene expression in potato. In the present study, four promoters derived from the potato genome have been characterized by the expression of identical cassettes carrying the fusion with the reporter β-glucuronidase (gusA) gene. The strengths of StUbi, StGBSS, StPat, and StLhca3 promoters were compared with the conventional constitutive CaMV 35S promoter in various organs (leaves, stems, roots, and tubers) of greenhouse-grown plants. The final amount of gene product was determined at the post-transcriptional level using histochemical analysis, fluorometric measurements, and Western blot analysis. The promoter strength comparison demonstrated that the StUbi promoter generally provided a higher level of constitutive β-glucuronidase accumulation than the viral CaMV 35S promoter. Although the StLhca3 promoter was predominantly expressed in a green tissue-specific manner (leaves and stems) while StGBSS and StPat mainly provided tuber-specific activity, a “promoter leakage” was also found. However, the degree of unspecific activity depended on the particular transgenic line and tissue. According to fluorometric data, the functional activity of promoters in leaves could be arranged as follows: StLhca3 > StUbi > CaMV 35S > StPat > StGBSS (from highest to lowest). In tubers, the higher expression was detected in transgenic plants expressing StPat-gusA fusion construct, and the strength order was as follows: StPat > StGBSS > StUbi > CaMV 35S > StLhca3. The observed differences between expression patterns are discussed considering the benefits and limitations for the usage of each promoter to regulate the expression of genes in a particular potato tissue.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Miroshnichenko

Фирсов

Timerbaev

et al. 2020

Plants

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“…However, when ubiquitous promoters are used for the expression of pleiotropic genes such as SlIAA9 or SlDELLA , they also cause unwanted phenotypes 18,22 . To overcome the disadvantages of these ubiquitous promoters, in recent years, many studies have reported the use of tissue-specific promoters in plant parts such as the seeds 23–25 , trichomes 26,27 , tubers 28 , roots 29,30 , pollen 31 , and petals 32 . However, research on promoters that drive the spatial and temporal expression of genes in reproductive tissues, especially in the ovary, are still rare.…”

Section: Introductionmentioning

confidence: 99%

Genetic engineering of parthenocarpic tomato plants using transient SlIAA9 knockdown by novel tissue-specific promoters

Kim

Ezura

Lee

et al. 2019

Sci Rep

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Parthenocarpy is the development of an ovary into a seedless fruit without pollination. The ubiquitous downregulation of SlIAA9 induces not only parthenocarpic fruit formation but also an abnormal vegetative phenotype. To make parthenocarpic transgenic tomato plants without unwanted phenotypes, we found two genes, namely, Solyc03g007780 and Solyc02g067760, expressed in ovary tissue but not in vegetative tissues. Solyc03g007780 was expressed in developing ovaries and anthers. Solyc02g067760 mRNA was detected in whole-flower tissues. The promoters of Solyc03g007780 (Psol80) and Solyc02g067760 (Psol60) predominantly induced the expression of genes in the ovule, placenta, endocarp and pollen and in whole-flower tissues, respectively. Psol80/60-SlIAA9i lines, created for SlIAA9-RNA interference controlled by two promoters, successfully formed parthenocarpic fruits without pleiotropic effects in vegetative tissues. Downregulation of SlIAA9, responsible for parthenocarpic fruit formation, was observed in ovules rather than ovaries in the Psol80/60-SlIAA9i lines. Although the weight of parthenocarpic fruits of the Psol80/60-SlIAA9i lines was lower than the weight of pollinated fruits of the wild type (WT), the parthenocarpic fruits presented redder and more saturated colors and higher levels of total soluble solids and titratable acidity than the WT fruits.

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In planta Agrobacterium-mediated transformation of L-Asparaginase gene into potato (Solanum tuberosum L.) cv. Agria: an efficient and novel method

Gharghi

Nazeri

Niazi‎

2022

Plant Biotechnol Rep

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The laccase promoter of potato confers strong tuber-specific expression in transgenic plants

Cited by 6 publications

References 53 publications

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potato

Genetic engineering of parthenocarpic tomato plants using transient SlIAA9 knockdown by novel tissue-specific promoters

In planta Agrobacterium-mediated transformation of L-Asparaginase gene into potato (Solanum tuberosum L.) cv. Agria: an efficient and novel method

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