The Lactic Acid Stress Response of Lactococcus lactis subsp. lactis

Hartke, Axel; Bouche, Sandrine; Giard, Jean‐Christophe; Benachour, Abdellah; Boutibonnes, P.; Auffray, Yanick

doi:10.1007/s002849900099

Cited by 129 publications

(98 citation statements)

References 17 publications

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“…Surprisingly, heat stimulation afforded protection against an acid challenge, but no cross-protection towards bile salts stress could be detected, whatever the temperature tested. These data underline the overlap of acid and heat regulons, as previously observed in other bacteria, whether they are Gram-positive [9], or negative [10]. However, heat stress, which was shown to confer cross-protection against bile salts stress in the Gram-positive Enterococcus faecalis [6], had on the contrary adverse effects on bile salts susceptibility in P. freudenreichii SI41.…”

Section: In Vitro Studysupporting

confidence: 73%

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Jan

Leverrier

Proudy

et al. 2002

Lait

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-Propionibacteria, which are largely used as Swiss-type cheese starters, also constitute promising human probiotics because of their beneficial effects on intestinal bifidobacteria and bacterial enzyme activities. To be efficient, they must survive acid stress imposed within the stomach and the surfactant effects of bile salts. We thus investigated the ability of Propionibacterium freudenreichii (strain Propiofidus ® SI41) to adapt to, and survive these stresses. In an in vivo study, 7 volunteers received Propiofidus ® SI41 in 3 different modes. The probiotic was supplied during 3 periods (i) in classical capsules at a low dose (5 × 10 9 CFU·d -1 ), (ii) in the same capsules at a higher dose (5 × 10 10 CFU·d -1 ), (iii) in acid-resistant capsules at the low dose. In the first case, propionibacteria were recovered above 10 5 CFU·g -1 in only 8 out of 14 faecal samples. In the two following cases, they were enumerated in all faecal samples between 10 5 and 10 7 CFU·g -1 . The recovery of live propionibacteria in faecal samples seemed to be related to a higher content in short chain fatty acids. This study, which clearly showed the role of gastric protection in the survival of propionibacteria, led us to investigate stress adaptation in this strain. Acid tolerance response was evidenced: transient exposure to pH 5 afforded protection towards acid challenge at pH 2. Moreover, we showed that pre-exposure to a moderate bile salts pre-treatment at 0.2 g·L -1 sharply increased its survival against a subsequent lethal challenge (1 g·L -1 ). Important changes in cellular protein synthesis were observed during adaptation using two-dimensional electrophoresis. Global protein synthesis decreased during adaptation treatments. In contrast, specific proteins were overexpressed during acid adaptation and others during bile salts adaptation. Among the up-regulated polypeptides, a carboxyl carrier protein (BCCP) and the recombinase RecR were identified during both stress responses while the universal chaperonins GroEL and GroES, as well as the replicase RepB, were only up-regulated during acid adaptation. These results bring new insights on the tolerance of P. freudenreichii to acid and bile salts, which should be taken into consideration for the development of probiotic preparations. Résumé -Survie et effets bénéfiques des bactéries propioniques dans l'intestin humain : étu-des in vivo et in vitro. Les propionibactéries, qui sont largement utilisées comme levain d'affinage des fromages à pâte pressée cuite, constituent également des probiotiques humains prometteurs du fait de leurs effets bénéfiques sur la flore bifide et les activités enzymatiques bactériennes, au niveau de l'intestin. Pour être efficaces, elles doivent survivre au stress acide subi dans l'estomac et à l'action détergente des sels biliaires. Nous avons donc étudié la capacité de Propionibacterium freudenreichii (souche Propiofidus ® SI41) à s'adapter et à survivre à ces stress. Dans une étude in vivo, 7 volontaires ont reçu Propiofidus ® SI41 selon 3 mo...

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Section: In Vitro Studysupporting

confidence: 73%

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Jan

Leverrier

Proudy

et al. 2002

Lait

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“…2, $ ). This high resistance was repeatedly found in more than 10 independent experiments with cultures in exponential growth phase, after the cells had been grown exponentially for more than eight generations to avoid entry into the stationary growth phase, which is known to increase the general resistance of Lactococci (Hartke et al, 1994).…”

Section: Ryssel and Othersmentioning

confidence: 72%

Multi-stress resistance in Lactococcus lactis is actually escape from purine-induced stress sensitivity

et al. 2014

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Multi-stress resistance is a widely documented and fascinating phenotype of lactococci where single mutations, preferentially in genes involved in nucleotide metabolism and phosphate uptake, result in elevated tolerance to multiple stresses simultaneously. In this report, we have analysed the metabolic basis behind this multi-stress-resistance phenotype in Lactococcus lactis subsp. cremoris MG1363 using acid stress as a model of multi-stress resistance. Surprisingly, we found that L. lactis MG1363 is fully resistant to pH 3.0 in the chemically defined SA medium, contrary to its sensitivity in the rich and complex M17 medium. When salvage of purines and subsequent conversion to GTP was permitted in various genetic backgrounds of L. lactis MG1363, the cells became sensitive to acid stress, indicating that an excess of guanine nucleotides induces stress sensitivity. The addition of phosphate to the acid-stress medium increased the stress sensitivity of L. lactis MG1363. It is also shown that high intracellular guanine nucleotide pools confer increased sensitivity to high temperatures, thus showing that it is indeed a multi-stress phenotype. Our analysis suggests that an increased level of guanine nucleotides is formed as a result of an improved conversion of guanosine in the salvage pathway. Based upon our findings, we suggest that L. lactis MG1363 is naturally multi-stress resistant in habitats devoid of any purine source. However, any exogenous purine that results in increased guanine nucleotide pools renders the bacterium sensitive to environmental stresses.

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“…These results suggest that lactic acid stress in L. plantarum WCFS1 also induces a more general stress response (as above described for different Lactobacillus species). An overlap between the stimulus for lactic acid and those for peroxide and UV radiation has also been reported for L. lactis [104,66]. The response of L. sanfranciscensis to osmotic stress (saccarose 40%) gives rise to the overproduction of 3-methylbutanoic acid and gamma-decalactones when L. sanfranciscensis was co-inoculated with yeasts, simulating a sourdough environment.…”

Section: General Steps Regarding a Virtual Fermented Food Processmentioning

confidence: 82%

“…DNA microarray technology has been used in numerous experiments to analyze gene expression: one example is the evaluation of the general stress response of B. subtilis [117] or the investigation of the transcription profiles of L. plantarum grown in steady-state cultures that varied in lactate/lactic acid concentration, pH, osmolarity [66,104]. This approach is useful also to study the behaviour of bacteria in a real food system.…”

Section: Methodological Approaches To Study the Effects Of Stress On Labmentioning

confidence: 99%

Dynamic Stresses of Lactic Acid Bacteria Associated to Fermentation Processes

Serrazanetti¹,

Gottardi²,

Montanari³

et al. 2013

Lactic Acid Bacteria - R &Amp; D for Food, Health and Livestock Purposes

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The Lactic Acid Stress Response of Lactococcus lactis subsp. lactis

Cited by 129 publications

References 17 publications

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Survival and beneficial effects of propionibacteria in the human gut: in vivo and in vitro investigations

Multi-stress resistance in Lactococcus lactis is actually escape from purine-induced stress sensitivity

Dynamic Stresses of Lactic Acid Bacteria Associated to Fermentation Processes

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