The Lactobacillus Crispatus M247 oral administration: it really is an effective strategy in the management of papillomavirus-infected women?

Abstract

-Background: Recent studies have shown the importance of the microbiota in women's health. In contrast, a dysbiosis condition can be a predisposing condition of viral infections by Human Papilloma Virus (HPV). The persistence of HPV-related lesions in patients with dysbiosis, could represent the antechamber of cervical cancer. The aim of this study is to evaluate whether long-term administration of oral lactobacilli may allow the restoration of eubiosis in women with HPV-infections with consequently viral clearance. -Methods: A total of 160 women affected HPV-infections were enrolled at Department of Gynecological Obstetrics of “San Paolo” Hospital, Italy between February 2021 and February 2022.Women were randomized in two groups, in treatment with oral Lactobacillus Crispatus M247 (group 1, n = 80) versus controlled group, with only follow-up (Group 2 n= 80).-Results: After a median follow up of 12 months (range 10-30 months) the chance to solve HPV-related cytological anomalies was higher in treatment with oral probiotic long-term users (group 1) versus group with placebo (group 2) (60.5% vs 41,3%, p = 0.05). Furthermore, a total HPV-clearance was shown in 9,3% in patients with only follow-up compared to a percentage of 15,3 % in group that assumed oral Lactobacilli long term users (p = 0.34). So, the percentual of patients assessed with HPV-DNA test documented at the end of the study period is not significatively different by the control group.-Conclusions: Despite the limitations of our analysis, we find a higher percentage of clearance of PAP-smear abnormalities, in patients who have took oral Lactobacilli long term than the control group. These results represent a confirm that the eubiosis re-establishment could be the first step to eradicate efficiently HPV-infection. Larger studies are need yet but we believe that future researchs should act in this direction.Trial registration: This word is retrospectively registered.

Effects of prebiotics, probiotics, and synbiotics on the prevention and treatment of cervical cancer: Mexican consensus and recommendations

“…Lactobacillus crispatus M247 is more frequent in non-infected women and was shown to have fecal and vaginal colonizing properties; according to a study by Dellino et al, oral administration with Lactobacillus crispatus was investigated in HPV-infected women. HPV clearance was 9.3% in controls vs. 15.3% in the probiotic group (8). Another study assessed the influence of probiotics on genital hrHPV clearance.…”

“…Gut microbiota influences the body's response to pathogens, including viral infections (5). Studies have demonstrated that certain probiotic strains can inhibit the growth of hrHPV, decrease viral load, and promote effector immune responses against the infection by enhancing the activity of immune cells, including natural killer cells and T cells, which play crucial roles in fighting cancer (6)(7)(8)(9)(10)(11). Thus, maintaining a balanced gut microbiome (eubiosis) may enhance the immune response against hrHPV and potentially reduce the risk of CC or improve antineoplastic response (4).…”

Human Metagenome Analysis with COVID-19 Infectious Disease

“…As a result of SARS-CoV-2 infection, there is a significant impact on the environment and dynamics of the human gut microbiome over the short and long term, which directly affects the health of the patient (13). The gut environment that is infected by the active SARS-CoV-2 virus and affected microbiome may also lead to an antagonistic environment, which facilitates the bloom of pathogenic bacteria and fungi, further limiting the formation and function of the gut microbiome, thereby crippling the immunity of the host (14).…”