The lamin B receptor of Drosophila melanogaster

Wagner, Nicole; Weber, Daniela; Seitz, Sabine; Krohne, Georg

doi:10.1242/jcs.01052

Cited by 64 publications

(51 citation statements)

References 48 publications

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“…Only depletion of Lem2 led to a detectable phenotype (see below). The absence of such a phenotype in cells depleted of the other INM proteins is in accordance with previous studies [10,11].…”

Section: Resultssupporting

confidence: 93%

The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology

et al. 2006

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The inner nuclear membrane (INM) of eukaryotic cells is characterized by a unique set of transmembrane proteins which interact with chromatin and/or the nuclear lamina. The number of identified INM proteins is steadily increasing, mainly as a result of proteomic and computational approaches. However, despite a link between mutation of several of these proteins and disease, the function of most transmembrane proteins of the INM remains unknown and depletion of many of these proteins from a variety of systems did not produce an obvious phenotype in the affected cells. Here, we report that depletion of the conserved INM protein Lem2 from human cell lines leads to abnormally shaped nuclei and severely reduces cell survival. We suggest that interactions of Lem2 with lamins or chromatin are critical for maintaining the integrity of the nuclear envelope.

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Section: Resultssupporting

confidence: 93%

The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology

et al. 2006

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“…Although Sf9 LBR has not been identified, the Drosophila melanogaster ortholog of human LBR (dLBR) has been characterized. The N-terminal region of dLBR binds to the insect lamin Dm0; however, binding of dLBR and Dmo is not essential for accumulation of dLBR in the INM (13). This is in contrast to other insect INM proteins (dMAN1, Bocksbeutel, and otefin), whose INM localizations are directly influenced by the presence of insect lamin Dm0 (14).…”

Section: Discussionmentioning

confidence: 84%

“…8 and 11). [4][5][6][7][8][9][10][11][12][13][14][15][16]. When microsomal membranes were prepared from cells infected with recombinant virus-expressing T7-tagged KPNA-4-16, the T7 antibody easily detected KPNA-4-16.…”

Section: Mammalian Cells Have a Counterpart To Sf9 Importin-␣-16 And Itmentioning

confidence: 99%

Early sorting of inner nuclear membrane proteins is conserved

Braunagel

Williamson²,

Ding

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…69 with some modifications, and immunostained by using rabbit polyclonal antibody against LamDm0 (no. 836) (70) or guinea pig polyclonal anti-dLBR antibody (71), and anti-DIG-FITCconjugated antibody (Roche). Secondary Alexa 546-or Alexa 647-conjugated antibodies were from Invitrogen.…”

Section: Drosophila Melanogaster Stocks Y W; Lam Sz18mentioning

confidence: 99%

The B-type lamin is required for somatic repression of testis-specific gene clusters

Shevelyov

Lavrov

Mikhaylova

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

123

118

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Large clusters of coexpressed tissue-specific genes are abundant on chromosomes of diverse species. The genes coordinately misexpressed in diverse diseases are also found in similar clusters, suggesting that evolutionarily conserved mechanisms regulate expression of large multigenic regions both in normal development and in its pathological disruptions. Studies on individual loci suggest that silent clusters of coregulated genes are embedded in repressed chromatin domains, often localized to the nuclear periphery. To test this model at the genome-wide scale, we studied transcriptional regulation of large testis-specific gene clusters in somatic tissues of Drosophila. These gene clusters showed a drastic paucity of known expressed transgene insertions, indicating that they indeed are embedded in repressed chromatin. Bioinformatics analysis suggested the major role for the B-type lamin, LamDmo, in repression of large testis-specific gene clusters, showing that in somatic cells as many as three-quarters of these clusters interact with LamDmo. Ablation of LamDmo by using mutants and RNAi led to detachment of testis-specific clusters from nuclear envelope and to their selective transcriptional up-regulation in somatic cells, thus providing the first direct evidence for involvement of the B-type lamin in tissue-specific gene repression. Finally, we found that transcriptional activation of the lamina-bound testis-specific gene cluster in male germ line is coupled with its translocation away from the nuclear envelope. Our studies, which directly link nuclear architecture with coordinated regulation of tissue-specific genes, advance understanding of the mechanisms underlying both normal cell differentiation and developmental disorders caused by lesions in the B-type lamins and interacting proteins.coexpressed genes ͉ nuclear lamina P revious studies by others and us have shown that many coexpressed tissue-specific genes are organized in large continuous clusters on chromosomes of diverse species (1-3), and similar clustering has been observed for the genes deregulated in diverse diseases (4-8). These findings imply that higherorder chromatin structure may be involved in regulation of extensive multigenic regions in both normal development and its pathological disruptions. In support of this suggestion, alterations in chromatin structure across large multigenic domains have been correlated to changes in gene expression (9-13). Repressed multigenic regions are frequently localized to nuclear periphery (14), and a number of studies linked derepression/ activation of genetic loci with their translocation away from the nuclear envelope (15-21). These observations suggest that tethering of genetic loci to nuclear lamina causes their silencing. Recent reports of repression of integrated transgenes and adjacent endogenous genes upon their artificial recruitment to the nuclear envelope (21, 22) support this hypothesis; however, similar studies on different loci did not show repression (23,24) indicating that silencing at nuclear ...

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The lamin B receptor of Drosophila melanogaster

Cited by 64 publications

References 48 publications

The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology

The inner nuclear membrane protein Lem2 is critical for normal nuclear envelope morphology

Early sorting of inner nuclear membrane proteins is conserved

The B-type lamin is required for somatic repression of testis-specific gene clusters

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