The Langendorff Heart

Dhein, Stefan

doi:10.1007/3-540-26574-0_9

Cited by 30 publications

(33 citation statements)

References 7 publications

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“…Calcium was added after oxygenation to prevent precipitation and the solution was filtered to 5 µm. O 2 saturation, pH and concentrations of Na, K, Ca were confirmed to be in the normal range before starting the experiment [33]. …”

Section: Methodsmentioning

confidence: 99%

Ultrasound Shear Wave Elasticity Imaging Quantifies Coronary Perfusion Pressure Effect on Cardiac Compliance

Vejdani‐Jahromi

Nagle

Trahey

et al. 2015

IEEE Trans. Med. Imaging

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Diastolic heart failure (DHF) is a major source of cardiac related morbidity and mortality in the world today. A major contributor to, or indicator of DHF is a change in cardiac compliance. Currently, there is no accepted clinical method to evaluate the compliance of cardiac tissue in diastolic dysfunction. Shear wave elasticity imaging (SWEI) is a novel ultrasound-based elastography technique that provides a measure of tissue stiffness. Coronary perfusion pressure affects cardiac stiffness during diastole; we sought to characterize the relationship between these two parameters using the SWEI technique. In this work, we demonstrate how changes in coronary perfusion pressure are reflected in a local SWEI measurement of stiffness during diastole. Eight Langendorff perfused isolated rabbit hearts were used in this study. Coronary perfusion pressure was changed in a randomized order (0–90 mmHg range) and SWEI measurements were recorded during diastole with each change. Coronary perfusion pressure and the SWEI measurement of stiffness had a positive linear correlation with the 95% confidence interval (CI) for the slope of 0.009–0.011 m/s/mmHg (R2 = 0.88). Furthermore, shear modulus was linearly correlated to the coronary perfusion pressure with the 95% CI of this slope of 0.035–0.042 kPa/mmHg (R2 = 0.83). In conclusion, diastolic SWEI measurements of stiffness can be used to characterize factors affecting cardiac compliance specifically the mechanical interaction (cross-talk) between perfusion pressure in the coronary vasculature and cardiac muscle. This relationship was found to be linear over the range of pressures tested.

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Section: Methodsmentioning

confidence: 99%

Ultrasound Shear Wave Elasticity Imaging Quantifies Coronary Perfusion Pressure Effect on Cardiac Compliance

Vejdani‐Jahromi

Nagle

Trahey

et al. 2015

IEEE Trans. Med. Imaging

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“…R ecording electrical signals from cells and tissue is central to areas ranging from the fundamental biophysical studies of function in, for example, the heart and brain, through medical monitoring and intervention (1)(2)(3). Over the past several decades, studies of electroactive cells have been carried out by using a variety of recording techniques, including glass micropipette intracellular and patch-clamp electrodes (1,3), voltage-sensitive dyes (4,5), multielectrode arrays (MEAs) (6)(7)(8), and planar FETs (9)(10)(11).…”

mentioning

confidence: 99%

“…Over the past several decades, studies of electroactive cells have been carried out by using a variety of recording techniques, including glass micropipette intracellular and patch-clamp electrodes (1,3), voltage-sensitive dyes (4,5), multielectrode arrays (MEAs) (6)(7)(8), and planar FETs (9)(10)(11). The latter 2 recording techniques use welldeveloped microfabrication methods to allow for direct multiplexed detection on a scale not possible with micropipette technology, although the MEAs and FETs exhibit limited signalto-noise ratios (S/N) and detection areas that make cellular and subcellular recording difficult (12,13).…”

mentioning

confidence: 99%

Flexible electrical recording from cells using nanowire transistor arrays

Cohen‐Karni

Timko²,

Weiss³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

212

207

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“…In a typical Langendorff preparation, the heart loses its functionality or becomes edematous at a rate of about 5-20% per hour. [12], [13] According to our results, normal systolic stiffness showed a 9% decrease and for diastolic stiffness it showed 11% increase. While during half-normal perfusion, diastolic stiffness increased and systolic stiffness decreased by 11% and 20% respectively.…”

Section: Discussionmentioning

confidence: 66%

The effect of acute coronary perfusion change on cardiac function measured by Shear Wave Elasticity Imaging

Vejdani‐Jahromi

Kiplagat

Trahey

et al. 2014

2014 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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The possibility of measuring cardiac function noninvasively has generated wide interest in elastography imaging techniques. Shear Wave Elasticity Imaging (SWEI) is an ultrasound-based elastography technique used to measure stiffness of tissues. While this technique has been studied extensively in static homogenous tissues such as liver, breast or prostate, there is still a significant need to study its capabilities to measure cardiac stiffness and function. In this research, we have studied the potential of SWEI to evaluate the coronary perfusion pressure effect on systolic and diastolic stiffness referred to as elastance and compliance of the heart. Five isolated rabbit hearts were used in this study in a Langendorff preparation. SWEI measurements of stiffness were recorded in two steps. In the first step, coronary perfusion was set to normal and then was reduced to half-normal. After 40 minutes of half-normal perfusion, it was returned to normal perfusion for the second step. SWEI velocity decreased from 6.003 m/s to 4.713 m/s in systole and from 1.948 m/s to 1.507 m/s in diastole in the first step. During the second step raising the perfusion to normal, SWEI stiffness showed an increase from 3.760 m/s to 5.468 m/s in systole and from 1.678 m/s to 2.156 m/s during diastole. Our results show that SWEI measurements of stiffness can characterize the cross talk between coronary perfusion and cardiac stiffness and also has the potential to measure compliance and elastance of the heart in systole and diastole.

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The Langendorff Heart

Cited by 30 publications

References 7 publications

Ultrasound Shear Wave Elasticity Imaging Quantifies Coronary Perfusion Pressure Effect on Cardiac Compliance

Ultrasound Shear Wave Elasticity Imaging Quantifies Coronary Perfusion Pressure Effect on Cardiac Compliance

Flexible electrical recording from cells using nanowire transistor arrays

The effect of acute coronary perfusion change on cardiac function measured by Shear Wave Elasticity Imaging

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