The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection

Dautigny, André; Pham-Dinh, Danielle; Roussel, Camille; Felix, J.M.; Nussbaum, J.L.; Jollès, Pierre

doi:10.1016/0006-291x(88)90254-9

Cited by 38 publications

(27 citation statements)

References 24 publications

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“…Synthesis of the neurofilament H mRNA was confirmed by Northern blot experiments; Fig. 4B shows that the cytoplasmic RNA hybridized with the neurofilament H-specific probe (40).…”

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confidence: 90%

“…Vimentin is preferentially expressed in various precursor cells (13), whereas the antibodies to GFAP recognize the astrocytes (44). y-Enolase (33) and neurofilaments (34,40,45) have been reported to be specifically expressed in neurons.…”

mentioning

confidence: 99%

“…Str.SVLT.3.8 cell line was examined for the expression of several receptors for neurotransmitters, coupled to adenylate cyclase (46,47), or to phospholipase C (42,48). As shown in Table 1, the 1-adrenergic receptors were revealed by stimulation of the cAMP generating system with the 83-adrenergic agonist isoproterenol (49 (20 ,ug) was hybridized with the EcoRI insert of pNF-H1 clone (40). Lanes: 1, RNA from mouse brain; 2 and 3, RNA from the cell line, grown in the N2 medium for 6 and 10 days, respectively.…”

mentioning

confidence: 99%

“…Most of them were derived from spontaneous or chemically induced tumors (14,15) or after transformation with oncogenic viruses (16,17). Some neural precursor cell line were obtained by transfection of the F9 embryonic carcinoma cell line with the simian virus 40 (SV40) large tumor (T) gene (18). Other lines, with astroglial properties, were derived from long-term primary cultures (19).…”

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confidence: 99%

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Immortalization of bipotential and plastic glio-neuronal precursor cells.

Evrard

Borde

Marin

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

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Permanent clonal cell lines from newborn mouse striatum have been established after transfer of the simian virus 40 large tumor oncogene by means of a retroviral vector. Some of the lines obtained displayed properties of bipotential and plastic glio-neuronal precursors. Depending on the culture conditions, these cells express either the glial fibrillary acidic protein or neuroframents. In addition, the cells can display adrenergic, D1 and D2 dopaminergic, muscarinic, and 5-hydroxytryptamine type 2 serotoninergic receptors, which are coupled either to the adenylate cyclase or to the phosphatidylinositol signaling pathways. The panel of receptors for neurotransmitters exhibited by these lines closely resembles that of primary striatal neurons. Results suggest that plastic common precursors of astrocytes and neurons persist in the striatum at a late developmental stage. As these permanent cell lines constitute an unlimited source of homogenous cell material, we suggest that they should be useful for molecular and pharmacological studies on the mechanisms and regulation of signal transduction as well as the commitment, plasticity, and differentiation of neural cells.

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“…Synthesis of the neurofilament H mRNA was confirmed by Northern blot experiments; Fig. 4B shows that the cytoplasmic RNA hybridized with the neurofilament H-specific probe (40).…”

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confidence: 90%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Immortalization of bipotential and plastic glio-neuronal precursor cells.

Evrard

Borde

Marin

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

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“…More recently, a partial cDNA clone for rat NF-H has been sequenced [14]. We have previously reported the isolation of a partial cDNA clone for rat NF-H [15] which extends over 200 bases further towards the N-terminal and here we describe the sequence of the clone which corresponds to approx.…”

Section: Introductionmentioning

confidence: 94%

Partial sequence of the rat heavy neurofilament polypeptide (NF‐H) Identification of putative phosphorylation sites

et al. 1988

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A 3 kb cDNA clone has previously been isolated in this laboratory corresponding to the rat heavy neurofilament polypeptide (NF-H). This clone, equivalent to approximately 70% of the total mRNA of the protein has been sequenced and shown to contain the carboxy-terminal region of the message. This contains 51 of the Lys-Ser-Pro repeat triplets which are reported to be the site of neurofilament phosphorylation. The sequence obtained was subsequently compared to those of mouse and human NF-H, showing a homology of approximately 85%. There is, however, one region which is variable between the species, this being the highly phosphorylated region of the protein containing the Lys-Ser-Pro triplet repeat.

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Developmental increases in expression of neurofilament mRNA selectively in projection neurons of the lamprey CNS

1996

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Neurofilaments of the sea lamprey are unique in being homopolymers of a single subunit (NF-180). Digoxigenin-labeled RNA probes complementary to NF-180 were used to determine the distribution and timing of expression of neurofilament message in the brain and spinal cord of the lamprey. In the brainstem, detection of NF-180 mRNA was restricted to neurons with axons projecting to the spinal cord or the periphery. The majority of brainstem neurons, whose axons project locally, did not express NF-180 within the detection limits of this technique. NF-180-positive neurons included cells with a wide range of axon diameters, suggesting neurofilament mRNA expression was linked to axon length rather than caliber. To further evaluate this hypothesis, expression was studied in animals of different developmental stages between larvae and adults. In younger (shorter) larvae, the large Mauthner and rhombencephalic Müller cells did not express NF-180 mRNA, even though their axons are among the largest caliber in the animal and extend the entire length of the spinal cord. In contrast, many other reticulospinal neurons, whose axons are smaller in diameter than those of the Müller and Mauthner cells, expressed NF-180 message throughout larval development. Furthermore, neurons of the cranial motor nuclei did not express NF-180 until later developmental stages and the extraocular motor neurons did not label until metamorphosis. Therefore, while detectable neurofilament mRNA expression in the lamprey is restricted to neurons with long axons, its expression in this population of neurons appears to be developmentally regulated by factors still not determined. It is postulated that need for NF message is determined by a balance between the volume of axon to be filled and the rate of turnover of NF in that axon.

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The large neurofilament subunit (NF-H) of the rat: cDNA cloning and in situ detection

Cited by 38 publications

References 24 publications

Immortalization of bipotential and plastic glio-neuronal precursor cells.

Immortalization of bipotential and plastic glio-neuronal precursor cells.

Partial sequence of the rat heavy neurofilament polypeptide (NF‐H) Identification of putative phosphorylation sites

Developmental increases in expression of neurofilament mRNA selectively in projection neurons of the lamprey CNS

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