The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis

Burton, Aisha T.; Kearns, Daniel B.

doi:10.1128/jb.00290-20

Cited by 16 publications

(10 citation statements)

References 133 publications

(199 reference statements)

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“…We then determined transformation frequencies and viability for these strains. As previously demonstrated, wild-type 3610 had an approximate transformation frequency of 10 -6 and curing the plasmid resulted in a 100-fold increase in transformation frequency (Figure 3B, black and dark blue) (10, 16). When comI was integrated on the chromosome in the cured strain, the transformation frequency was similar to the cured strain alone (Figure 3B, dark and light blue).…”

Section: Resultssupporting

confidence: 72%

“…These systems rely on sensing elements created by the phage genome and respond by activating their killing module, in this case ComI. Secondly, nearly half the genes on pBS32 encode for a putative prophage, and it has been speculated that the entire plasmid could be a prophage phagemid (16). Thus, this model could represent a phage defense system in response to DNA uptake.…”

Section: Discussionmentioning

confidence: 99%

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ComI inhibits transformation inBacillus subtilisby selectively killing competent cells

Smith,

Kearns,

Burton

2024

Preprint

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Many bacteria build elaborate molecular machines to import DNA via natural competence, yet this activity is often not identified until strains have been handled and domesticated in laboratory settings. For example, one of the best studied Gram-positive model organisms, Bacillus subtilis, has a non-transformable ancestor. Transformation in the ancestral strain is inhibited by a transmembrane peptide, ComI, which is encoded on an extrachromosomal plasmid. Although ComI was shown to be necessary and sufficient to inhibit transformation when produced at high levels under an inducible promoter, the mechanism by which ComI inhibits transformation is unknown. Here, we examine the native regulation and mechanism of transformation inhibition by ComI. We find that under native regulation, ComI expression is restricted in the absence of the plasmid. In the presence of the plasmid, we find that ComI is preferentially expressed in cells that are differentiating into a competent state. The subcellular localization of ComI, however, does not depend on any other competence proteins and permeabilization activity is concentration dependent. Thus over time, the competent cells gradually producing ComI, are permeabilized and killed. Based on these observations we propose a new model for the mechanism of ComI, suggesting a response to competence activation that selectively eliminates the competent subpopulation.

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Section: Resultssupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

ComI inhibits transformation inBacillus subtilisby selectively killing competent cells

Smith,

Kearns,

Burton

2024

Preprint

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“…It has been established that microbial interactions, including horizontal genetic exchange, signaling, and metabolite exchange, occur between microorganisms in biofilm communities [1,[7][8][9]. It has been found that within a biofilm, bacteria of different taxa form a single genetic system in the form of plasmids and other MGE that carry a behavioral code for members of the biofilm and determine their trophic, energy, and other connections between themselves and the outside world [2,4,9,10].…”

Section: Introductionmentioning

confidence: 99%

“…However, experimental studies of this plan are few, especially when pathogenic enterobacteria and marine bacteria are co-cultivated. In this regard, studies seem to be very significant, allowing us to establish: to what extent the marine environment is a "cemetery" of microorganisms and to what time it is a "bridgehead" for the formation of epidemic variants of pathogenic bacteria [7,10].…”

Section: Introductionmentioning

confidence: 99%

Horizontal Transfer of Virulence Factors by Pathogenic Enterobacteria to Marine Saprotrophic Bacteria during Co-Cultivation in Biofilm

Eskova

Andryukov

Yakovlev

et al. 2022

BioTech

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Environmental problems associated with marine pollution and climate warming create favorable conditions for the penetration and survival of pathogenic bacteria in marine ecosystems. These microorganisms have interspecific competitive interactions with marine bacteria. Co-culture, as an important research strategy that mimics the natural environment of bacteria, can activate silent genes or clusters through interspecies interactions. The authors used modern biotechnology of co-cultivation to dynamically study intercellular interactions between different taxa of bacteria—pathogenic enterobacteria Yersinia pseudotuberculosis and Listeria monocytogenes and saprotrophic marine bacteria Bacillus sp. and Pseudomonas japonica isolated in summer from the coastal waters of the recreational areas of the Sea of Japan. The results of the experiments showed that during the formation of polycultural biofilms, horizontal transfer of genes encoding some pathogenicity factors from Y. pseudotuberculosis and L. monocytogenes to marine saprotrophic bacteria with different secretion systems is possible. It was previously thought that this was largely prevented by the type VI secretion system (T6SS) found in marine saprotrophic bacteria. The authors showed for the first time the ability of marine bacteria Bacillus sp. and P. japonica to biofilm formation with pathogenic enterobacteria Y. pseudotuberculosis and L. monocytogenes, saprophytic bacteria with type III secretion system (T3SS). For the first time, a marine saprotrophic strain of Bacillus sp. Revealed manifestations of hyaluronidase, proteolytic and hemolytic activity after cultivation in a polycultural biofilm with listeria. Saprotrophic marine bacteria that have acquired virulence factors from pathogenic enterobacteria, including antibiotic resistance genes, could potentially play a role in altering the biological properties of other members of the marine microbial community. In addition, given the possible interdomain nature of intercellular gene translocation, acquired virulence factors can be transferred to marine unicellular and multicellular eukaryotes. The results obtained contribute to the paradigm of the epidemiological significance and potential danger of anthropogenic pollution of marine ecosystems, which creates serious problems for public health and the development of marine culture as an important area of economic activity in coastal regions.

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“…Його результати представлені на таблицях та генетичних картах (таблиці 4.14, 4.15; рисунки 4.14, 4.16, 4.19).Характеристики секвенованих геномів добре укладаються в діапазон значень окремих ознак, властивих відповідним видам. Так, B. velezensis ONU 553 та B. subtilis ONU 559 позбавлені плазмід, що відомо для приблизно 80% штамів цих видів[27,54], а штам B. pumilus ONU 554 має плазміду, як це характерно для пpедставникiв виду[48].…”

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Аеробні Споротвірні Бактерії Глибоководних Осадів Чорного Моря

Штеніков¹,

Іваниця²

2021

Мікробіологічні Дослідження Чорного Моря

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З глибоководних відкладень Чорного моря виділено ізоляти споротвірних бактерій, які методом аналізу профілів жирних кислот віднесено до 14 видів родів Bacillus, Paenibacillus та Brevibacillus. Виділені бактерії охарактеризовані за антагоністичною активністю по відношенню до опортуністичних патогенів людини. За результатами скринінгу було відібрано Bacillus velezensis ONU 553, B. pumilus ONU 554та B. subtilis ONU 559. Загальні характеристики геномів цих бактерій укладаються в діапазон значень, властивий відповідним видам. Методом рідинної хромато-масспектрометрії у бактерій штаму Bacillus velezensis ONU553 виявлено та ідентифіковано 90, B. pumilus ONU 554 – 33, B. subtilis ONU 559 - 43 екзометаболіти. Серед них виявлено антибіотики класів ліпопептидів та амікумацинів, а також пентапептид GPFPI. Виявлені сполуки, які ідентифіковані для бацил вперше, потребують подальшої верифікації. Для ряду сполук вперше ідентифіковано біосинтетичні генні кластери. Розраховані за профілями жирних кислот індекси термофільності та аналіз амінокислотних спектрів за методом Moura (в авторській модифікації) вказують на неаборигенність штамів B. velezensis ONU 553 та B. subtilis ONU 559 для донних відкладень. Виділені і вивчені штами морських бактерій поповнили Колекцію морських мікроорганізмів Одеського національного університету імені І.І. Мечникова, а отримані дані щодо геномів та екзометаболомів морських бактерій можуть слугувати науковою основою для розробки нових фармацевтичних препаратів.

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The Large pBS32/pLS32 Plasmid of Ancestral Bacillus subtilis

Cited by 16 publications

References 133 publications

ComI inhibits transformation inBacillus subtilisby selectively killing competent cells

ComI inhibits transformation inBacillus subtilisby selectively killing competent cells

Horizontal Transfer of Virulence Factors by Pathogenic Enterobacteria to Marine Saprotrophic Bacteria during Co-Cultivation in Biofilm

Аеробні Споротвірні Бактерії Глибоководних Осадів Чорного Моря

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