The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size

Mambro, Riccardo Di; Svolacchia, Noemi; Ioio, Raffaele Dello; Pierdonati, Emanuela; Salvi, Elena; Pedrazzini, Emanuela; Vitale, Alessandro; Perilli, Serena; Sozzani, Rosangela; Benfey, Philip N.; Busch, Wolfgang; Costantino, Paolo; Sabatini, Sabrina

doi:10.1016/j.cub.2019.02.022

Cited by 75 publications

(83 citation statements)

References 49 publications

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“…Its position is controlled by regulatory balance of auxin and cytokinine [45], which positions the auxin minimum [47] initiating the cell elongation (i.e., the differentiation). The regulation of auxin accumulation, and its degradation through ARR1 signaling, is dependent on PIN5 and GH3.17 genes [48]. A shortened meristematic zone with rapid transition from basal portion of proliferation domain into transition domain is one of developmental possibilities that lead to shorter primary root in ahl18.…”

Section: Root Apical Meristem and Ahl18mentioning

confidence: 99%

At-Hook Motif Nuclear Localised Protein 18 as a Novel Modulator of Root System Architecture

Širl

Šnajdrová

Gutiérrez-Alanís

et al. 2020

IJMS

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The At-Hook Motif Nuclear Localized Protein (AHL) gene family encodes embryophyte-specific nuclear proteins with DNA binding activity. They modulate gene expression and affect various developmental processes in plants. We identify AHL18 (At3G60870) as a developmental modulator of root system architecture and growth. AHL18 is involved in regulation of the length of the proliferation domain and number of dividing cells in the root apical meristem and thereby, cell production. Both primary root growth and lateral root development respond according to AHL18 transcription level. The ahl18 knock-out plants show reduced root systems due to a shorter primary root and a lower number of lateral roots. This change results from a higher number of arrested and non-developing lateral root primordia (LRP) rather than from a decreased LRP initiation. The over-expression of AHL18 results in a more extensive root system, longer primary roots, and increased density of lateral root initiation events. AHL18 is thus involved in the formation of lateral roots at both LRP initiation and their later development. We conclude that AHL18 participates in modulation of root system architecture through regulation of root apical meristem activity, lateral root initiation and emergence; these correspond well with expression pattern of AHL18.

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Section: Root Apical Meristem and Ahl18mentioning

confidence: 99%

At-Hook Motif Nuclear Localised Protein 18 as a Novel Modulator of Root System Architecture

Širl

Šnajdrová

Gutiérrez-Alanís

et al. 2020

IJMS

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“…Our results show increases in both PIN3 and LAX1 proteins in auxin‐treated seedlings (average ratio values 1.66 and 1.95, respectively, Figure D), which are consistent with previously reported expression data from imaging proPIN3::PIN3‐GFP lines and ProLAX1:GUS reporter lines . It has also recently been shown that GH3.17 protein levels are crucial for maintenance of auxin levels in root meristems, suggesting that there may be positive feedback regulation on this protein regulate auxin homeostasis.…”

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confidence: 99%

Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings

et al. 2019

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It is known that auxin induces rapid gene expression changes throughout plant development, but how these transcriptional responses relate to changes in protein abundance is not well characterized. This report identifies early auxin responsive proteins in whole Arabidopsis seedlings using an isobaric tags for relative and absolute quantification‐based quantitative proteomics approach. Approximately 25% of the detected proteins (1045 out of 4257 proteins) are auxin responsive, which is in line with the central role of auxin in the regulation of plant growth and development. Several well‐known auxin pathway proteins are identified as differentially expressed, validating this quantitative proteomics approach. Additionally, functional categorization of these auxin responsive proteins indicates that rapid and complex metabolic changes occur in seedlings in response to auxin, including lipid metabolism. Altogether, these data describe novel auxin‐regulated proteins and are an excellent resource for identifying new downstream signaling components related to auxin‐mediated plant growth and development.

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“…Based on our data, we do not exclude the role of EXPA1 in the control of RAM size, but probably in a concert with other EXPAs and dominantly in the columella/LRC (vide infra). The role of (cytokinin-regulated) auxin accumulation in the LRC in the control of RAM size has been proposed recently (Di Mambro et al, 2019). However, even in case of more distinct transcriptional regulation of EXPA1 by NAA, we do not see any significant and consistent EXPA1 upregulation in the LRC, both at the level of promoter activity or EXPA1 protein (data not shown), thus leaving the functional importance of cytokinin-and auxin-mediated regulation over EXPA1 in the root tip rather unclear.…”

Section: Discussionmentioning

confidence: 99%

Expansin-controlled cell wall stiffness regulates root growth inArabidopsis

Samalova

Elsayad

Melnikava

et al. 2020

Preprint

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19Expansins facilitate cell expansion via mediating pH-dependent cell wall (CW) loosening. 20However, the role of expansins in the control of biomechanical CW properties in the tissue and 21 organ context remains elusive. We determined hormonal responsiveness and specificity of 22 expression and localization of expansins predicted to be direct targets of cytokinin signalling. We 23 found EXPA1 homogenously distributed throughout the CW of columella/ lateral root cap, while 24 EXPA10 and EXPA14 localized predominantly at the three-cell boundaries of epidermis/cortex in 25 various root differentiation zones. Expression of EXPA15, revealing cell type-specific localization 26 pattern, overlaps with higher CW stiffness measured via Brillouin light scattering microscopy. As 27 indicated by both higher Brillouin frequency shift and AFM-measured Youngs' modulus, EXPA1 28 overexpression upregulated CW stiffness, associated with shortening of the root apical meristem 29 and root growth arrest. We propose that root growth in Arabidopsis requires delicate orchestration 30 of biomechanical CW properties via tight regulation of various expansins' localization to specific 31 cell types and extracellular domains. 32

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The Lateral Root Cap Acts as an Auxin Sink that Controls Meristem Size

Cited by 75 publications

References 49 publications

At-Hook Motif Nuclear Localised Protein 18 as a Novel Modulator of Root System Architecture

At-Hook Motif Nuclear Localised Protein 18 as a Novel Modulator of Root System Architecture

Auxin Induces Widespread Proteome Remodeling in Arabidopsis Seedlings

Expansin-controlled cell wall stiffness regulates root growth inArabidopsis

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