The LcKNAT1-LcEIL2/3 Regulatory Module Is Involved in Fruitlet Abscission in Litchi

Ma, Xingshuai; Ying, Peiyuan; He, Zidi; Wu, Hong; Li, Jianguo; Zhao, Minglei

doi:10.3389/fpls.2021.802016

Cited by 6 publications

(4 citation statements)

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“…It is generally accepted that ethylene triggers IDA‐HAE/HSL2 signaling, which in turn accomplishes the last steps in the abscission process (Botton & Ruperti, 2019; Estornell et al., 2015; Li et al., 2021; Ma et al., 2022; Meir et al., 2019; Sto et al., 2015; Tucker & Yang, 2012; Wang et al., 2019; Wilmowicz et al., 2018; Ying et al., 2016). Previously, we found that LcACO2 , LcACS1 , and LcEIL3 are involved in litchi fruitlet abscission (Ma et al., 2019; Ma, Yuan, et al., 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, we observed that the expression of LcIDL1 was increased in LcDOF5.6‐silenced FAZ, and both LcIDL1 and LcHSL2 were decreased in LcRbohD‐silenced FAZ (Figure 8), suggesting that LcDOF5.6–LcRbohD‐mediated ROS has a positive effect on the LcIDL1‐LcHSL2 signaling in regulating the litchi fruitlet abscission. Considering that ethylene could also act upstream of IDA‐HAE/HSL2 signaling, which in turn accomplish the final stages of organ abscission (Botton & Ruperti, 2019; Ma et al., 2022; Meir et al., 2019), the activation of IDA signaling might be achieved by the ROS‐triggered ethylene signaling.…”

Section: Discussionmentioning

confidence: 99%

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A LcDOF5.6–LcRbohD regulatory module controls the reactive oxygen species‐mediated fruitlet abscission in litchi

Xie

et al. 2023

The Plant Journal

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SUMMARY Reactive oxygen species (ROS) have been emerging as a key regulator in plant organ abscission. However, the mechanism underlying the regulation of ROS homeostasis in the abscission zone (AZ) is not completely established. Here, we report that a DOF (DNA binding with one finger) transcription factor LcDOF5.6 can suppress the litchi fruitlet abscission through repressing the ROS accumulation in fruitlet AZ (FAZ). The expression of LcRbohD, a homolog of the Arabidopsis RBOHs that are critical for ROS production, was significantly increased during the litchi fruitlet abscission, in parallel with an increased accumulation of ROS in FAZ. In contrast, silencing of LcRbohD reduced the ROS accumulation in FAZ and decreased the fruitlet abscission in litchi. Using in vitro and in vivo assays, we revealed that LcDOF5.6 was shown to inhibit the expression of LcRbohD via direct binding to its promoter. Consistently, silencing of LcDOF5.6 increased the expression of LcRbohD, concurrently with higher ROS accumulation in FAZ and increased fruitlet abscission. Furthermore, the expression of key genes (LcIDL1, LcHSL2, LcACO2, LcACS1, and LcEIL3) in INFLORESCENCE DEFICIENT IN ABSCISSION signaling and ethylene pathways were altered in LcRbohD‐silenced and LcDOF5.6‐silenced FAZ cells. Taken together, our results demonstrate an important role of the LcDOF5.6–LcRbohD module during litchi fruitlet abscission. Our findings provide new insights into the molecular regulatory network of organ abscission.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A LcDOF5.6–LcRbohD regulatory module controls the reactive oxygen species‐mediated fruitlet abscission in litchi

Xie

et al. 2023

The Plant Journal

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“…Specific FaEIN3/EINs showed high expression levels at the initial red stage of the strawberry fruit in a certain cultivar, suggesting they might play a role during fruit ripening. Also, Ma et al [61,62] proposed that LcEIL2 and LcEIL3 regulated ethylene-activated litchi fruitlet abscission by binding to LcCEL2/8 and LcPG1/2 in a cell wall remodeling process and LcACS1/4/7 and LcACO2/3 in the ethylene biosynthetic pathway. Mu et al (2016) [57] demonstrated that the expression levels of most of the genes involved in ethylene biosynthesis and signaling had the same changing trend in non-climacteric fruits (grape and strawberry): they reached a maximum in the fruit development and then decreased, indicating that the mechanism of ethylene production and perception occurs in these fruits prior to ripening.…”

Section: Discussionmentioning

confidence: 99%

Ethylene Signaling Pathway Genes in Strawberry and Their Expression Patterns during Fruit Ripening

Zhang

Deng

et al. 2023

Agronomy

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Ethylene at least partly regulates some aspects during non-climacteric ripening in strawberry. However, the ethylene signaling pathway genes in the strawberry fruit have not been comprehensively and systematically analyzed. In the present study, 15 FaETRs and 14 FaEIN3/EINs were identified in the octoploid strawberry genome. Subcellular localization analysis predicted that FaETRs and FaEIN3/EINs are respectively localized to the endoplasmic reticulum and the nucleus. The phylogenetic trees showed that FaETRs were classified into two subgroups, while FaEIN3/EINs were divided into three clades, which was supported by gene structure and conserved motif analysis. FaETRs and FaEIN3/EINs could interact with several components, such as CTR1, RTE1, EIN2 and ERF1B, in the ethylene signaling pathway by protein–protein interaction network analysis. Transcriptomic data showed that FaETRs were mainly expressed at the early stage of fruit development in three strawberry cultivars. Additionally, a couple of FaETRs (FaETR2 and FaETR13) and FaEINs (FaEIN2 and FaEIN7) could be induced by 1 μM ABA and inhibited by 100 μM nordihydroguaiaretic acid (NDGA, an ABA biosynthesis blocker). These findings suggested that the FaETR- and FaEIN3/EIN-mediated ethylene signaling pathway might play a role in strawberry fruit ripening.

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“…The development and functional exercise of the abscission layer is a complex and precise process in which multiple enzymes ( Tong et al, 2020 ; Wang et al, 2023 ), hormones ( Kućko, Wilmowicz & Ostrowski, 2019 ; Li et al, 2023 ), and genes ( Wang et al, 2021 ; Ma et al, 2022 ) are involved in regulation. Abscission is a physiological process associated with stress response and plant organ senescence.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome differential expression analysis of defoliation of two different lemon varieties

Dong,

Yin,

Gao

et al. 2024

PeerJ

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‘Allen Eureka’ is a bud variety of Eureka lemon with excellent fruiting traits. However, it suffers from severe winter defoliation that leads to a large loss of organic nutrients and seriously affects the tree’s growth and development as well as the yield of the following year, and the mechanism of its response to defoliation is still unclear. In order to investigate the molecular regulatory mechanisms of different leaf abscission periods in lemon, two lemon cultivars (‘Allen Eureka’ and ‘Yunning No. 1’) with different defoliation traits were used as materials. The petiole abscission zone (AZ) was collected at three different defoliation stages, namely, the pre-defoliation stage (CQ), the mid-defoliation stage (CZ), and the post-defoliation stage (CH). Transcriptome sequencing was performed to analyze the gene expression differences between these two cultivars. A total of 898, 4,856, and 3,126 differentially expressed genes (DEGs) were obtained in CQ, CZ, and CH, respectively, and the number of DEGs in CZ was the largest. GO analysis revealed that the DEGs between the two cultivars were mainly enriched in processes related to oxidoreductase, hydrolase, DNA binding transcription factor, and transcription regulator activity in the defoliation stages. KEGG analysis showed that the DEGs were concentrated in CZ and involved plant hormone signal transduction, phenylpropanoid biosynthesis, glutathione metabolism, and alpha-linolenic acid metabolism. The expression trends of some DEGs suggested their roles in regulating defoliation in lemon. Eight gene families were obtained by combining DEG clustering analysis and weighted gene co-expression network analysis (WGCNA), including β-glucosidase, AUX/IAA, SAUR, GH3, POD, and WRKY, suggesting that these genes may be involved in the regulation of lemon leaf abscission. The above conclusions enrich the research related to lemon leaf abscission and provide reliable data for the screening of lemon defoliation candidate genes and analysis of defoliation pathways.

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The LcKNAT1-LcEIL2/3 Regulatory Module Is Involved in Fruitlet Abscission in Litchi

Cited by 6 publications

References 46 publications

A LcDOF5.6–LcRbohD regulatory module controls the reactive oxygen species‐mediated fruitlet abscission in litchi

A LcDOF5.6–LcRbohD regulatory module controls the reactive oxygen species‐mediated fruitlet abscission in litchi

Ethylene Signaling Pathway Genes in Strawberry and Their Expression Patterns during Fruit Ripening

Transcriptome differential expression analysis of defoliation of two different lemon varieties

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