Currently, there are four unique generations of employees on the labour market (Generation BB, Generation X, Generation Y, and Generation Z. The individual performance of employees is largely influenced by their motivation to perform. The main aim of the article is to present the results of the research focused on the analysis of the performance motivation of different generations in Slovak industrial enterprises. A standardized psychodiagnostic performance motivation questionnaire (D-M-V) was used for data collection, which was distributed to six industrial enterprises operating in Slovakia. The research sample consisted of N=158 respondents (administrative employees and managers). By evaluating the established research hypotheses, statistically, significant relationships were identified between the monitored variables from the D-M-V questionnaire and perceived age discrimination and the possibility of further education in industrial enterprises. Proven and quantified statistically significant relationships can be used to increase individual employee performance, which contributes to the overall performance of the organization.