“…We performed cladistic analyses of sequence and indel data from two highly variable, non‐coding DNA regions: nuclear ribosomal ITS region (including the internal transcribed spacers, ITS1 and ITS2, and the intervening 5.8S gene; Baldwin et al ., 1995) and plastid trnL/trnF region (encompassing the intron of trnL , the intergenic spacer between trnL and trnF and partial exon portions; Taberlet et al ., 1991). Both these regions have succeeded, alone or in combination with one another or with other DNA regions, in resolving intra‐ and intergeneric phylogenetic relationships in Spiranthinae (Salazar et al ., 2003; Górniak et al ., 2006; Figueroa et al ., 2008; Salazar & Ballesteros‐Barrera, 2010; Batista et al ., 2011; Salazar & Dressler, in press) and other orchidoid lineages (e.g. Bellstedt, Linder & Harley, 2001; Kores et al ., 2001; Clements et al ., 2002; Bateman et al ., 2003; van der Niet et al ., 2005; Álvarez‐Molina & Cameron, 2009; Salazar et al ., 2009).…”