The Legislative Regulation of Biologically Active Food Supplements Circulation in the Russian Federation

Tutelyan, V A; Sukhanov, Boris P.

doi:10.1016/b978-012373901-8.00020-2

Cited by 2 publications

(5 citation statements)

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“…Ash content was determined according to [ 19 ]. Squalene content was determined according to [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…The samples were extracted with 60% aqueous methanol in an ultrasonic bath at room temperature for 30 min, then centrifuged, and the supernatant was analyzed. Saponins were identified by retention times and mass spectra in accordance with [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

“…The amino acid composition was determined using a Hitachi automatic amino acid analyzer, followed by computer data processing using Multichrome software for Windows. Preparation of samples was carried out according to the method of Moore and Stein [ 20 ]. The samples dehydrated with acetone and defatted with sulfuric ether were hydrolyzed with a 6 N hydrochloric acid solution for 24 h at a temperature of 105 °C.…”

Section: Methodsmentioning

confidence: 99%

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Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate

Sidorova

Петров

Перова

et al. 2023

Foods

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Special attention is being paid to the study of amaranth proteins. They are characterized by a high biological value that significantly exceeds those of grain crops. The production of protein concentrate from amaranth flour includes preliminary enzymatic hydrolysis, extraction of the resulting mixture, protein precipitation, microfiltration, and freeze-drying. In our study, the obtained amaranth protein concentrate was limited by valine, with an amino acid score of 74%. The true digestibility of the amaranth protein concentrate determined in vivo was 97.6 ± 0.3%, which was significantly lower than that of casein (99.3 ± 0.2%). The protein digestibility-corrected amino acid score value of the concentrate was 72.2%. The obtained concentrate was a rich source of selenium, copper, magnesium, manganese, and iron. Ferulic acid was the only polyphenolic compound found in the amaranth protein concentrate, but its content was significantly greater compared to the original flour. Saponins were not removed completely during the process of obtaining the amaranth protein concentrate. We identified 15 saponins in the concentrate, mainly of the bidesmoside type, the sapogenins of which are related derivatives of oleanolic acid. Thus, the developed amaranth protein concentrate can be used as an ingredient in functional food products, with a high biological value.

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“…Ash content was determined according to [ 19 ]. Squalene content was determined according to [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate

Sidorova

Петров

Перова

et al. 2023

Foods

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“…The content of total polyphenols was determined by the Folin-Ciocalteu method [12]. Gallic acid was used as a standard.…”

Section: Determination Of Total Polyphenol Contentmentioning

confidence: 99%

“…The profile of triterpene saponins was determined by RP HPLC-MS on an Ultimate 3000 liquid chromatography system (Dionex, USA) with a diode array spectrophotometric detector (DAD) and a TSQ Endura triple quadrupole mass spectrometric detector (Thermo Fisher Scientific, USA). Saponins were identified by retention times and mass spectra in accordance with [12].…”

Section: Determination Of Triterpene Saponins Contentmentioning

confidence: 99%

Application of assisted extraction technologies to optimize the extraction of biologically active substances from plant materials

et al. 2023

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There is an increased interest in obtaining amaranth grain extracts enriched with biologically active substances by deep processing of raw materials with a further prospect of their use as part of specialized multi-purpose food products. The aim of this work was to evaluate the effectiveness of membrane filtration for the extraction of biologically active substances from amaranth grains. Amaranth grain extract was obtained by enzymatic hydrolysis using a proteolytic enzyme followed by membrane treatment. the developed approach made it possible to obtain an extract from amaranth grains - a source of biologically active peptides, 20-hydroxyecdysone and polyphenols (including saponins). In total, triterpene saponins were concentrated in the extract by 8 times, and the phytoecdysteroid 20-hydroxyecdysone was more than 30 times concentrated. The high content of low molecular weight peptide fractions (more than 80%) in the composition of the extract makes it promising to evaluate its antihypertensive and antidiabetic properties in vivo. The presence of 20-hydroxyecdysone in the extract, an adaptogen with a proven effect, opens up the possibility of its use as a functional food ingredient in specialized food products, including for athletes.

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The Legislative Regulation of Biologically Active Food Supplements Circulation in the Russian Federation

Cited by 2 publications

References 0 publications

Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate

Physical and Chemical Characterization and Bioavailability Evaluation In Vivo of Amaranth Protein Concentrate

Application of assisted extraction technologies to optimize the extraction of biologically active substances from plant materials

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