2008
DOI: 10.1016/j.biocel.2007.10.034
|View full text |Cite
|
Sign up to set email alerts
|

The lens epithelium: Focus on the expression and function of the α-crystallin chaperones

Abstract: Lens epithelial cells are the parental cells responsible for growth and development of the transparent ocular lens. Many elegant investigations into their biology have focused on the factors that initiate and regulate lens epithelial cell differentiation. Because they serve key transport and cell maintenance functions throughout life, and are the primary source of metabolic activity in the lens, mechanisms to maintain lens epithelial cell integrity and survival are critical for lens transparency. The molecular… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

1
60
0
1

Year Published

2009
2009
2024
2024

Publication Types

Select...
5
3

Relationship

0
8

Authors

Journals

citations
Cited by 74 publications
(62 citation statements)
references
References 32 publications
1
60
0
1
Order By: Relevance
“…Measurement of Apoptosis-Human lens epithelial cells (HLE) 2 were isolated from the lens of a 44-year-old donor and cultured in DMEM supplemented with 10% FBS as previously described for mouse lens epithelial cells (27). Cells between passages 5 and 6 were used for the experiments.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Measurement of Apoptosis-Human lens epithelial cells (HLE) 2 were isolated from the lens of a 44-year-old donor and cultured in DMEM supplemented with 10% FBS as previously described for mouse lens epithelial cells (27). Cells between passages 5 and 6 were used for the experiments.…”
Section: Methodsmentioning
confidence: 99%
“…It is composed of two subunits, ␣A and ␣B, whose sequences show significant homology to each other and to other small heatshock proteins (1). The ␣A-and ␣B-crystallin heteropolymers are present in the lens as polydisperse oligomers with an average molecular mass of 800 kDa (2,3). ␣A-Crystallin is present mainly in the lens, whereas ␣B-crystallin, in addition to the lens, is present in several other tissues, including the retina, heart, and kidney (4,5).…”
mentioning
confidence: 99%
“…However, the lens contains several nonenzymatic and enzymatic mechanisms to protect itself from oxidative stress and to repair oxidatively damaged cell components. The nonenzymatic mechanisms include the presence of high concentrations of ascorbate, reduced glutathione (GSH), crystallins (which act as protein chaperones), and free UV filters (e.g., tryptophan derivatives in the human lens and NAD(P)H in rabbit, guinea pig and frog lenses (2,16,125,127).…”
Section: Ros Degradation/inactivation Systemsmentioning
confidence: 99%
“…The ocular lens has a unique cellular architecture consisting of a single layer of cuboidal epithelial cells, which divide and differentiate at the equator into fiber cells (1). The fiber cells elongate, and they synthesize fiber cell-specific proteins, such as AQP0 (aquaporin-0)/MIP (main intrinsic protein), cytoskeletal proteins, and crystallins (1)(2)(3)(4).…”
mentioning
confidence: 99%
“…The fiber cells elongate, and they synthesize fiber cell-specific proteins, such as AQP0 (aquaporin-0)/MIP (main intrinsic protein), cytoskeletal proteins, and crystallins (1)(2)(3)(4). As the new fiber cells are laid down at the lens equator, the older fiber cells are pushed toward the lens core and simultaneously lose their nuclei and organelles while exhibiting very little protein turnover.…”
mentioning
confidence: 99%