The lethal protein from kintoki beans (Phaseolus vulgaris) identified as a lectin.

Miyoshi, Masamitsu; Nakabayashi, Junko; Hara, Tomoko; Yawata, Takiko; Tsukamoto, Ikuyo; Hamaguchi, Yoichi

doi:10.3177/jnsv.28.255

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…As presumed from our previous data (13,14) and other investigators' data (4,18,19) indicating the presence of antinutritive factors in raw beans, whole Kintoki bean when orally ingested was proved to have a toxic effect on mice and rats. Mice fed a diet containing autoclaved Kintoki bean at a 10% protein level increased their body weight 10% in nine days, and their motion was also normal in experiment 1.…”

Section: And Discussionmentioning

confidence: 56%

“…Considering the binding properties of lectin to the epithelial cells (21) and the decrease of the rates of intestinal absorption/digestion of main nutrients in rats, the intestine is possibly regarded as the first and probably the main target tissue of orally fed lectin. When mice were injected the Kintoki bean lectin intraperitoneally, congestion occurred in the liver, lung and spleen (14) . Table 2 shows the tissue weight of mice fed 40mg lectin by stomach-feeding on an albumin diet in experiment 2 and of rats in experiment 3.…”

Section: And Discussionmentioning

confidence: 99%

“…We already purified a lectin from Phaseolus vulgaris Kintoki bean and characterized it chemically and confirmed that the intraperitoneal or intravenous injection of the Kintoki bean lectin causes death of mice in a short period (13,14) . Therefore, it is important to elucidate the mechanisms of the toxic effects caused by lectin fed orally.…”

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confidence: 99%

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Oral toxicity of Kintoki bean (Phaseolus vulgaris) lectin.

Hara

Tsukamoto

Miyoshi

1983

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThere are several antinutritive factors in the Kintoki bean such as lectin, trypsin inhibitor, lack of methionine etc. The present experiment has revealed that lectin is mainly responsible for the growth impairment of experimental animals orally fed raw Kintoki bean. Mice fed raw Kintoki bean as the only protein source lost their body weight and died in 8 days, while mice fed the heated bean grew normally. When mice on a 10% albumin diet ingested 20mg or 40mg or 60mg Kintoki bean lectin by daily stomach-feeding, their body weights were reduced to 84%, 74%, 71% of the control group after 5 days respectively and some of them could not live to complete the experiment. The apparent rates of the intestinal absorption of carbohydrate, lipid, and protein were considerably reduced, when rats were fed a diet containing 0.4% lectin. Especially, the rate of protein absorption was decreased to 26.3% from 55.5% of the control rate. The main tissues of mice that had ingested Kintoki bean lectin by stomach-feeding were subjected to microscopic observation. No changes were observed in the liver, kidney, spleen and pancreas. But in the small intestine, the epithelial cells lining the villi were considerably disordered and conspicuously disrupted. These results indicate that the Kintoki bean lectin is one of the most promoting factors for growth impairment in experimental animals and that the first target organ in the case of oral feeding is the small intestine. Key Words toxic lectin, oral toxicity, Phaseolus vulgaris bean, growth impairment, disruption of the villi Bean protein is expected to cover exceedingly the protein deficiency which people are now facing in the world (1, 2). It has been recognized, however, that the nutritive values of legumes are very poor unless they are subjected to cooking or 589

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Section: And Discussionmentioning

confidence: 56%

Section: And Discussionmentioning

confidence: 99%

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Oral toxicity of Kintoki bean (Phaseolus vulgaris) lectin.

Hara

Tsukamoto

Miyoshi

1983

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…The differences of extraction methods to cause break up or crowd for molecules were effective also in activity of extraction [12]. According to the our results give the higher inhibition percent in Liver GOT at higher doses compared to Cisplatin which recorded the higher inhibition at the same dose, could be attributed to Gurde extract was mixture of many chemical compounds may be their to overlap cause to inhibition one of these compounds, Miyoshi et al ., [13] were explain sugar finding lead to change to lactine structure cause to inhibit of stimulated act [14] .…”

Section: -Lungmentioning

confidence: 99%

Study of cytotoxic Effects Alcoholic Nerium Oleander L. Extract on female Albino mice

Razzaq¹

2011

Baghdad Sci.J

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This study involved the evaluation of Alcoholic extract of Nerium Oleander L. plant that have a promising anticancer cell. This extract was compared to the well known anticancer drug Cis – Platinum by utilizing an in vivo system in female Albino mice. The first direction was cytogenetically using the mitotic Index of bone marrow cells as a parameter for the cytotoxic effect of this extract. The second direction was enzymatical using a widely distributed enzyme GOT in the different organs of mice: Liver , kidney , spleen and lung . Animals were treated with three doses of Cis-platin , 50 , 200 and 350 Mg/mouse for three days . The same doses were used for the other extract . This study showed that the extract have a promising anticancer cell as could be seen from these effect on mitotic Index (MI) of mice bone marrow , (MI) decreased in animals treated with different doses of extract , mitotic index was reduced to 78% on day three in animals treated with 350 ?g/mouse . These effects were similar to the effect of Cis-platin at the same doses. Comparing the effect of this extract on GOT enzyme showed that Cis-platin was more effective on activating the spleen GOT enzyme of about 95% than the extract while the extract is more effective in Lung , The extract activated GOT enzyme activity in the all organs.

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“…Although moist-heat treatment eliminates toxicity, lectins show resistance to denaturation during food processing (Aricigil & Pryme, 2015). It has also been reported that HA activity diminishes drastically to undetectable levels during the heating processes (cooking and autoclaving; Miyoshi et al, 1982;Shimelis & Rakshit, 2007). Lectins are resistant to digestion, survive in the gut, and are absorbed during endocytosis (Pusztai & Bardocz, 1996).…”

Section: Introductionmentioning

confidence: 99%

Antiproliferative and anti‐inflammatory effects of fractionated crude lectins from boiled kidney beans (Phaseolus vulgaris)

Une,

Nakata,

Nonaka

et al. 2023

Journal of Food Science

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In this study, we investigated the biological profile of lectins isolated from raw and boiled Japanese red Kintoki beans (red kidney beans [RKB]; Phaseolus vulgaris). Lectins in beans showing agglutination activity were retained after heating. Raw and boiled RKB lectins were fractionated using carboxymethyl‐ and diethylaminoethyl‐Sepharose, respectively. Boiled RKB lectins were evaluated for carbohydrate specificity as well as cytokine‐inducing and antiproliferative activities against cancer cells and compared with raw RKB lectins. Raw RKB lectins showed specificity for thyroglobulin and fetuin, whereas boiled lectins showed specificity for N‐acetylneuraminic acid. Raw RKB lectins showed low resistance to proteases and tolerated temperatures greater than 80°C for 5 min. Notably, lectins from raw and boiled beans showed antiproliferative activity against five types of cancer cells B16, LM8, HeLa, HepG2, and Colo 679. In particular, lectins from raw beans exhibited a significantly stronger activity than those from boiled beans. Anti‐inflammatory effects were notably observed in crude extracts from raw and boiled beans. Specifically, lectins fractionated from boiled beans markedly inhibited the expression of tumor necrosis factor‐α and interleukin‐6. Overall, our results showed that RKB lectins from boiled beans exert anti‐inflammatory and anticancer effects and could be developed as potential chemopreventive agents.Practical ApplicationJapanese red kidney beans (RKB) are cultivated in numerous parts of the temperate zone and consumed in many countries. Lectins from boiled beans exhibited anticancer activity, similar to lectins from raw beans. Additionally, crude and fractionated lectins from boiled beans showed anti‐inflammatory activity. Thus, boiled RKB lectins have the potential to be used as a bioactive protein for medical research and could be developed as anticancer agents.

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The lethal protein from kintoki beans (Phaseolus vulgaris) identified as a lectin.

Cited by 9 publications

References 13 publications

Oral toxicity of Kintoki bean (Phaseolus vulgaris) lectin.

Oral toxicity of Kintoki bean (Phaseolus vulgaris) lectin.

Study of cytotoxic Effects Alcoholic Nerium Oleander L. Extract on female Albino mice

Antiproliferative and anti‐inflammatory effects of fractionated crude lectins from boiled kidney beans (Phaseolus vulgaris)

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