The leuX-encoded tRNA5Leu but not the pathogenicity islands I and II influence the survival of the uropathogenic Escherichia coli strain 536 in CD-1 mouse bladder mucus in the stationary phase

Abstract

The uropathogenic Escherichia coli strain 536 carries two pathogenicity islands, each of which is associated with either of the tRNA genes selC or leuX, respectively. Growth competition in CD-1 mouse mucus between the wild-type strain E. coli 536, its leuX mutant 536v102 and its mutant 536R3, lacking both pathogenicity islands but expressing a functional tRNA S veu , revealed a major impact of leuX on E. coli survival in bladder mucus. The impaired survival in CD-1 mouse mucus observed upon deletion of the leuX gene was abolished after complementation with the leuX gene. The survival of bacteria in bladder mucus was not influenced by the presence of pathogenicity islands I and II. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

LeuX tRNA‐dependent and ‐independent mechanisms of Escherichia coli pathogenesis in acute cystitis

“…LeuX is unusual among tRNA genes in that it is regulated by the stress factor RpoH (Dobrindt and Hacker, 2001). Furthermore, leuX expression was previously shown to be important for stationary‐phase survival in bladder mucus (Dobrindt et al ., 1998), an important extracellular phenotype of UPEC. In contrast, our studies suggest roles for tRNA 5 Leu during invasion, intracellular survival, and rapid intracellular proliferation.…”

Beneficial Microorganisms in Multicellular Life Forms

“…These iron binding systems are additional to the enterobactin iron binding system which is also present in avirulent strains such as E. coli K-12 (Babai et al 1997;Blum-Oehler et al 2000;Carniel 2001;Hacker et al 2003;Oelschlaeger et al 2002a). The genes involved in virulence are often located on PAIs ¼ pathogenicity islands and there are extensive evidence for the occurrence of lateral gene transfer of these genes and these PAIs (Bach et al 2000;Bingen-Bidois et al 2002;Blum-Oehler et al 2000;Blum et al 1994;Brzuszkiewicz et al 2006Brzuszkiewicz et al , 2009Buchrieser et al 1999;Carniel et al 1996;Dezfulian et al 2004;Dobrindt et al 1998Dobrindt et al , 2000Dobrindt et al , 2002Dobrindt et al , 2003Dozois and Curtiss 1999;Finlay and Falkow 1997;Hacker et al 1992Hacker et al , 1997Hacker et al , 2003Kaper 1999, 2000;Houdouin et al 2002;Johnson et al 2001;Morschhauser et al 1994;Oelschlaeger et al 2002b;Rakin et al 1999;Redford and Welch 2002;Ritter et al 1995Ritter et al , 1997Schneider et al 2004;Schubert et al 1999). There is a high degree of variability in virulence factors of ExPEC strains.…”