The level of enzymes involved in the allantoin metabolism of Bacillus fastidiosus grown under different conditions

Muruke, Masoud Hadi; Camp, Huub J. M. Op den; Semesi, A. K.; Drift, Chris van der

doi:10.1007/bf00294523

“…There have been efforts to isolate bacteria and screen fungi whose enzymes are of medicinal, industrial and environmental importance with the chief aim of isolating and characterizing them (Muruke et al, 1993(Muruke et al, , 1995(Muruke et al, , 1996Akhmanova et al, 1998). Growth of Bacillus fastidiosus in a medium containing glycerol as a carbon source and urea as a nitrogen source leads to production of glycerol kinase, glycerol 3-phosphate, dehydrogenase, allantoinase, urease and carboligase under batch and continuous culture conditions.…”

Section: Production Of Enzymes Of Economic Importancementioning

confidence: 99%

Trends in industrial and environmental biotechnology research in Tanzania

Mtui¹

2007

Afr. J. Biotechnol.

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This paper reviews the trends in industrial and environmental biotechnology research in Tanzania for the past 20 years. Researches on production of bio-energy, enzymes and organic acids are reviewed. Furthermore, researches related to wastewater treatment systems including water stabilization ponds and constructed wetlands are covered. Brief highlights are made on researches related to bioprospecting and molecular biology techniques used in identification of organisms. In critically reviewing the research done so far, an attempt is made to pinpoint significant research gaps in areas such as single cell protein, biomining, biocomposting and bioengineering. This paper, therefore, provides an overview of the renewable raw materials available in the developing countries and outlines the researches that have been done to convert them to bio-products, while reducing bio-wastes. The identified gaps will serve as guidance to scientists who are interested in doing research in untapped areas of biotechnology.

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“…Research on production and purification of enzymes of economic importance has drawn a lot of interest. There have been efforts to isolate bacteria and screen fungi whose enzymes are of medicinal, industrial and environmental importance with the chief aim of isolating and characterizing them (Muruke et al, 1993(Muruke et al, , 1995(Muruke et al, , 1996Akhmanova et al, 1998). Growth of Bacillus fastidiosus in a medium containing glycerol as a carbon source and urea as a nitrogen source leads to production of glycerol kinase, glycerol 3-phosphate, dehydrogenase, allantoinase, urease and carboligase under batch and continuous culture conditions.…”

Section: Production Of Enzymes Of Economic Importancementioning

confidence: 99%

Biotechnology research

N.J.¹,

J.K.²,

G.³

2003

Afr. J. Biotechnol.

5

0

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This paper reviews the trends in industrial and environmental biotechnology research in Tanzania for the past 20 years. Researches on production of bio-energy, enzymes and organic acids are reviewed. Furthermore, researches related to wastewater treatment systems including water stabilization ponds and constructed wetlands are covered. Brief highlights are made on researches related to bioprospecting and molecular biology techniques used in identification of organisms. In critically reviewing the research done so far, an attempt is made to pinpoint significant research gaps in areas such as single cell protein, biomining, biocomposting and bioengineering. This paper, therefore, provides an overview of the renewable raw materials available in the developing countries and outlines the researches that have been done to convert them to bio-products, while reducing bio-wastes. The identified gaps will serve as guidance to scientists who are interested in doing research in untapped areas of biotechnology.

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“…We aligned the amino acid sequences of 16 amidohydrolases including -hydantoinases, dihydropyrimidinases, allantoinases and dihydro-orotases. Microbial -hydantoinases [15,[24][25][26] were revealed to share about 37-42 % amino acid identity with mammalian dihydropyrimidinases [9,10] and similarity increased up to 65 % when the conservative substitutions were considered, which implies that microbial -hydantoinase is much more closely related to animal dihydropyrimidinase than allantoinase and dihydro-orotase. Thus this result strongly supports the suggestion that the microbial -hydantoinase is a counterpart of animal dihydropyrimidinase.…”

Section: Alignment Of Amino Acid Sequences Of the Functionally Relatementioning

confidence: 99%

“…Characteristics of dihydro-orotases from mammalian multifunctional protein CAD [11] and Escherichia coli [12] have been reported. Allantoinase, the first enzyme involved in the degradation of allantoin, which is an intermediate in the catabolic pathway for purines, was studied from the eukaryotes [13,14] and micro-organisms [15]. However, little attention has been paid to the structure-function relationship and catalytic mechanism of the functionally related amidohydrolases at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Identification of the structural similarity in the functionally related amidohydrolases acting on the cyclic amide ring

Kim

¹

,

Kim

²

1998

Biochemical Journal

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The functionally related amidohydrolases, including D-hydantoinases, dihydropyrimidinases, allantoinases and dihydro-orotases, share a similar catalytic function of acting on the cyclic amide ring. We aligned 16 amidohydrolases by taking account of the conservative substitution and found a number of highly conserved regions and invariant amino acid residues. Analyses of the secondary structure and hydropathy profile of the enzymes revealed a significant degree of similarity in the conserved regions. Among the regions, the long stretched region I is of particular interest, because it is mainly composed of invariant amino acid residues, showing a similarity of 69% for the enzymes. A search of the protein data bank using the sequence of the conserved region I identified a number of proteins possessing a similar catalytic property, providing a clue that this region might be linked with the catalytic function. As a particular sequence, one aspartic acid and four histidine residues are found to be rigidly conserved in the functionally related amidohydrolases. In order to investigate the significance of the conserved residues, site-directed mutagenesis was carried out typically for the D-hydantoinase gene cloned from Bacillus stearothermophilus SD1. These residues were found to be essential for metal binding as well as catalysis, strongly implying that these invariant residues play a critical role in other enzymes as well as in D-hydantoinase. On the basis of the similar catalytic function and existence of the rigidly conserved sequence, we propose a close evolutionary relationship among the functionally related amido hydrolases, including D-hydantoinase, dihydropyrimidinase, allantoinase and dihydroorotase.

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The level of enzymes involved in the allantoin metabolism of Bacillus fastidiosus grown under different conditions

Cited by 4 publications

References 10 publications

Trends in industrial and environmental biotechnology research in Tanzania

Trends in industrial and environmental biotechnology research in Tanzania

Biotechnology research

Identification of the structural similarity in the functionally related amidohydrolases acting on the cyclic amide ring

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