The level of sugars and synthesis of trehalose in<i>Ascaris suum</i>tissues

Dmitryjuk, Małgorzata; Łopieńska–Biernat, Elżbieta; Farjan, Marek

doi:10.1017/s0022149x08165178

Cited by 13 publications

(17 citation statements)

References 37 publications

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“…The contents of fructose, trehalose, maltotriose, and saccharose were determined by HPLC. The protocols for sample preparation and analysis were based on the procedures described by Dmitryjuk et al (2009). Before HPLC, extracts were boiled for 5 min, two volumes of ethanol were added, and the probes were centrifuged after 15 min.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Dietary Vitamin C on Carbohydrate Concentrations and Hydrolase Activity, During the Development of Honey Bee Worker Brood

Farjan

Zoltowska

Lipiński

et al. 2015

Journal of Apicultural Science

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A b s t r a c t The colony collapse disorder is a growing problem world-wide. For this reason, we were prompted to search for natural and harmless agents that could improve the living conditions of honey bees. This group of agents includes exogenous antioxidants, such as ascorbic acid, which boost natural immunity. We analysed the effect of vitamin C supplementation on carbohydrate metabolism in the developing honey bee worker brood. The total carbohydrate content and the concentrations of glycogen, trehalose, maltotriose, fructose, and glucose were estimated. The correlations between sugar content and the activity of the main hydrolases of carbohydrate metabolism -α-amylase, glucoamylase, trehalase, maltase, and sucrase -were determined. The addition of vitamin C to the diet of wintering bees did not impair their sugar metabolism. Vitamin C supplements exerted a positive effect by signifi cantly increasing glycogen and trehalose concentrations in the initial phase of development and in newly emerged workers. Vitamin C did not induce signifi cant changes in the developmental profi le of carbohydrate degrading enzymes, except for the earliest stage of larval development when enzyme activity levels were below those noted in the control group.

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Section: Methodsmentioning

confidence: 99%

The Effect of Dietary Vitamin C on Carbohydrate Concentrations and Hydrolase Activity, During the Development of Honey Bee Worker Brood

Farjan

Zoltowska

Lipiński

et al. 2015

Journal of Apicultural Science

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“…The activity of TPS and TPP was observed in A. suum tissues, excluding the cuticle and intestines (Dmitryjuk et al 2009). T6P synthase was isolated, and two genes encoding TPS -tps1 (jF412033.2) and tps2 (jF412034.2) -were sequenced from the muscles of A. suum (Dmitryjuk et al 2013).…”

Section: Trehalose-6-phosphate Phosphatase (Tpp)mentioning

confidence: 95%

“…Trehalose, the end-product of enzymatic activity, was assayed by high-performance liquid chromatography (HPLC) according Dmitryjuk et al (2009).…”

Section: Enzyme Assaysmentioning

confidence: 99%

The gene expression and the activity of enzyme synthesis of trehalose during development ofAscaris suum(Nematoda) eggs

Dmitryjuk

Łopieńska–Biernat

2016

Invertebrate Reproduction & Development

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The activity of trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) and their mRNA expression was determined in Ascaris suum eggs developing from a zygote to invasive larvae in an in vitro culture. The stages of nematode embryonic development, i.e. cleavage, gastrulation and formation of larvae, were always accompanied by an increase in TPS activity. An increase in TPP activity, the second enzyme of the analysed pathway, followed in subsequent developmental stages. Key changes in trehalose metabolism were observed in the tadpole stage. The development of moving larvae was accompanied by the highest mRNA expression and activity level of TPP, which converts metabolic intermediate T6P to trehalose. The trehalose synthesis enzymes activity was positively correlated with their mRNA expression. For three genes: tps1, tps2 (TPS coding) and tpp (TPP coding) there was very strong and statistically significant correlation with the activity of a specific enzyme. The Pearson's coefficient was r = 0.903714 (p < 0.001) for tps1 gene, r = 0.803012 ( < 0.01) for tps2 and r = 0.856244 (p < 0.01) for tpp over development time. © 2016 Informa uK limited, trading as taylor & Francis Group KEYWORDS trehalose-6-phosphate synthase; trehalose-6-phosphate phosphatase; tps1; tps2 and tpp genes ARTICLE HISTORY

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“…Determination of soluble carbohydrates by means of high performance liquid chromatography (HPLC) Subsamples of 200 µl of hemolymph were taken for analysis, performed according to Dmitryjuk et al (2009). Carbohydrate chromatography was carried out in a SCL-10A chromatograph equipped with a RID-10A refractometric detector (Shimadzu), on a 250 × 4.6 mm High-Performance Carbohydrate cartridge column, at 55…”

Section: Total Carbohydrate Content Determinationmentioning

confidence: 99%

Total protein and carbohydrate content and protease and disaccharidase activities in the hemolymph of Lymnaea stagnalis naturally infected with digenean larvae

et al. 2013

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Lymnaea stagnalis is an intermediate host of many Digenea. The infestation affects host metabolism. The aim of the work was to investigate hemolymph biochemical indicators of L. stagnalis infected with four species of trematodes: Diplostomum pseudospathaceum, Paryphostomum radiatum, Plagiorchis elegans or Opisthioglyphe ranae. The protein profiles and proteinase activity in the hemolymph of sexually mature individuals of Lymnaea stagnalis maintained at 19 • C were tested. As the carbohydrates are main substrates for energetic metabolism of the great pond snail their content and disaccharidase activity were also studied. Hemolymph samples were collected during weeks 3 and 4 of rearing. No significant differences in the total protein content between uninfected individuals and snails infected with the first three trematode species were detected. In the snails infected with O. ranae the quantity of total proteins was near twice higher than in those uninfected. A higher share of 70 kDa proteins in infected than in uninfected snails as well as reduction of the low molecular weight fractions of proteins for snails infected with D. pseudospathaceum and P. radiatum were detected. During week 3, carbohydrate content in the infected snails did not differ from that in the controls while during week 4 it was significantly lower in snails infected with P. elegans or O. ranae. The content of the major soluble carbohydrate in the hemolymphsaccharose -changed in a similar way. No activity of trypsin or pepsin in the hemolymph sample was detected while the activity of chymotrypsin was lower in infected snails vs. controls. On the other hand, saccharase and maltase activities were higher in infected than in uninfected snails. The biochemical hemolymph indicators in naturally infected host-snails show some differences depending on the parasite species but they are not sufficiently species-specific to offer the basis for establishing the model unique for a particular parasitosis. Our results from the field did not always coincide with those from the laboratory.

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The level of sugars and synthesis of trehalose inAscaris suumtissues

Cited by 13 publications

References 37 publications

The Effect of Dietary Vitamin C on Carbohydrate Concentrations and Hydrolase Activity, During the Development of Honey Bee Worker Brood

The Effect of Dietary Vitamin C on Carbohydrate Concentrations and Hydrolase Activity, During the Development of Honey Bee Worker Brood

The gene expression and the activity of enzyme synthesis of trehalose during development ofAscaris suum(Nematoda) eggs

Total protein and carbohydrate content and protease and disaccharidase activities in the hemolymph of Lymnaea stagnalis naturally infected with digenean larvae

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