The levels of hepatocyte growth factor in serum and follicular fluid and the expression of c-Met in granulosa cells in patients with polycystic ovary syndrome

Şahin, Neslın; Toylu, Asli; Gülekli, Bülent; Dogan, Erbil; Kovalı, Müge; Atabey, Neşe

doi:10.1016/j.fertnstert.2012.08.059

Cited by 11 publications

(2 citation statements)

References 26 publications

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“…NGF may have an important role in the ovary with excessive production being associated with cystic ovarian morphology in rodent models akin to that found in women with PCOS 31 32 . HGF is an adipokine that is also found in the ovary 33 but has been associated with obesity and metabolic syndrome 34 35 36 with the suggestion that it has importance in insulin resistance associated compensatory mechanisms leading to an increase in insulin secretion through enhanced beta cell mass 35 . Furthermore, of those identified, molecular mechanisms of cancer was common for both miRNAs.…”

Section: Discussionmentioning

confidence: 99%

Increased expression of circulating miRNA-93 in women with polycystic ovary syndrome may represent a novel, non-invasive biomarker for diagnosis

Sathyapalan

David

Gooderham

et al. 2015

Sci Rep

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MicroRNAs (miRNA) are a novel class of small noncoding single-stranded RNA molecules that regulate gene expression. There is increasing evidence of their importance in polycystic ovary syndrome (PCOS). The objective was to determine if miRNA-93 and miRNA-223 are differentially expressed in the circulation of women with PCOS compared to age matched women. A case–control study comparing women with PCOS (n = 25) to age and weight matched controls (n = 24) without PCOS was performed. MiRNA-93 and miRNA-223 were determined by total RNA reverse transcription. Both miRNA-93 and miRNA-223 were significantly increased relative to the control group (p < 0.01, p = 0.029 respectively). In both groups there was no correlation of either miRNA-93 or miRNA-223 with insulin, HOMA-IR, HOMA-β or testosterone levels. The area under the receiver operator characteristic curve for miR-223 and miR-93 was 0.66 and 0.72 respectively, suggesting miR-93 is a more efficient biomarker than miR-223 for diagnosis of PCOS. The combination of the two miRNAs together, tested using multiple logistic regression analysis, did not improve the diagnostic potential. In conclusion, circulating miRNA-93 and miRNA-223 were higher in women with PCOS compared to age and weight matched controls independent of insulin resistance and testosterone levels, and miR-93 may represent a novel diagnostic biomarker for PCOS.

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Section: Discussionmentioning

confidence: 99%

Increased expression of circulating miRNA-93 in women with polycystic ovary syndrome may represent a novel, non-invasive biomarker for diagnosis

Sathyapalan

David

Gooderham

et al. 2015

Sci Rep

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“…This anoikis resistance activity as well as the mitogenic activity of FF was not conferred by IGF-1R and was largely or partially contributed by AKT. Other growth factors in FF, such as platelet-derived growth factor (PDGF) [36], hepatocyte growth factor (HGF) [50] and fibroblast growth factor-basic (bFGF) [51], may transmit these proliferative and survival signals.…”

Section: Discussionmentioning

confidence: 99%

Ovulatory Follicular Fluid Facilitates the Full Transformation Process for the Development of High-Grade Serous Carcinoma

Hsu

Chen

Seenan

et al. 2021

Cancers

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Background: High-grade serous carcinoma (HGSC) is mainly derived from the stepwise accumulation of driver mutations in the fallopian tube epithelium (FTE), and it subsequently metastasizes to the ovary and peritoneum that develops into a clinically evident ovarian carcinoma. The developmental process involves cell proliferation/clonal expansion, cell migration, anoikis resistance, anchorage-independent growth (AIG), peritoneum attachment, and cell invasion. Previously, we discovered FTE could be transformed by follicular fluid (FF) released from ovulation, the most crucial risk factor of ovarian cancer, and IGF axis proteins in FF confers stemness activation and clonal expansion via IGF-1R/AKT pathway. However, whether other phenotypes in advanced cancer development are involved is unknown. Methods: A panel of FTE and ovarian HGSC cell lines with different severity of transformation were treated with FF with or without IGF-1R and AKT inhibitors and analyzed for the transformation phenotypes in vitro, ex vivo, and in vivo. Results: FF largely promotes (by order of magnitude) cell migration, AIG, cell invasion, peritoneum attachment, anoikis resistance, and cell proliferation. Most of these activities worked in the full panel of cell lines. The AIG activity largely depends on IGF-1R/AKT phosphorylation, and the proliferation activity depends on an AKT phosphorylation not mediated by IGF-1R. In contrast, both AKT- and non-AKT-mediated signals are responsible for the other transformation activities. Conclusions: Our data demonstrate an extensive transformation activity of FF in the full journey of carcinogenesis, and endorsed ovulation-inhibition for the prevention and AKT-inhibition for the treatment of ovarian HGSC.

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PCOS and Insulin Resistance (IR): From Lifestyle to Insulin Sensitizers

et al. 2015

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The levels of hepatocyte growth factor in serum and follicular fluid and the expression of c-Met in granulosa cells in patients with polycystic ovary syndrome

Cited by 11 publications

References 26 publications

Increased expression of circulating miRNA-93 in women with polycystic ovary syndrome may represent a novel, non-invasive biomarker for diagnosis

Increased expression of circulating miRNA-93 in women with polycystic ovary syndrome may represent a novel, non-invasive biomarker for diagnosis

Ovulatory Follicular Fluid Facilitates the Full Transformation Process for the Development of High-Grade Serous Carcinoma

PCOS and Insulin Resistance (IR): From Lifestyle to Insulin Sensitizers

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