The levels of metalloproteineses and their endogenic inhibitors in patients with laryngeal cancer

Klisho, E. V.; Савенкова, О. В.; Кондакова, И. В.; Перельмутер, В. М.; Чойнзонов, Е. Л.; Shishkin, D. A.

doi:10.1016/s0167-8140(07)80083-1

Cited by 2 publications

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“…Similar to the results obtained by others, we have not found a correlation between MMP-9 expression in cancerous tissue in tumor stroma and the T stage of the TNM classification. Some researchers [22–24] have shown a positive correlation between MMP-9 expression and metastases in regional lymph nodes. Other studies [12, 13, 25, 26] similar to ours did not confirm such correlation.…”

Section: Discussionmentioning

confidence: 99%

“…In the relevant literature, we have not found any studies where the MMP-9 concentration in serum or plasma obtained in an isolated laryngeal carcinoma group was measured using immunoenzymatic assays. Klisho et al [24] have examined MMP-9 and TIMP-1 activity in serum obtained from patients with that localization of tumor and revealed statistically significant differences in TIMP-1 but not in MMP-9 activity between the study and the control groups. They did not find a significant correlation between MMP-9 and TIMP-1 activity, consistent with our study results.…”

Section: Discussionmentioning

confidence: 99%

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Expression and Concentration of Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinases 1 in Laryngeal Squamous Cell Carcinoma

et al. 2019

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The aim of this study was to assess the expression of MMP-9 and TIMP-1 in cancerous tissue as well as in the serum and plasma concentrations of these proteins in patients with laryngeal cancer and compare the results to the inflammatory reaction in healthy subjects. Twenty-seven patients who were diagnosed with laryngeal carcinoma and selected for total laryngectomy were included in the study group. MMP-9 and TIMP-1 expression in tissues was assessed using immunohistochemical assays. Immunoenzymatic ELISA methods were used to measure MMP-9 and TIMP-1 concentrations in serum and plasma. MMP-9 and TIMP-1 were identified in tumor cells and in the tumor stroma compartment, as well as in macroscopically healthy mucous membrane. MMP-9 expression was more significant in tumor stroma than in the perimatrix of the mucous membrane (p=0.047). TIMP-1 expression was significantly higher in the matrix and perimatrix of the mucous membrane than in cancer tissue (p=0.0093) and the tumor stroma compartment (p<0.0001). Expression of TIMP-1 was observed more frequently in tumors without infiltrated lymph nodes (p=0.009). Serum concentrations of MMP-9 and TIMP-1 as well as plasma TIMP-1 concentration were significantly higher in the study group than in the control group (p=0.0004, p=0.002, and p=0.0001, respectively). A significantly higher TIMP-1 level in plasma was found in patients with poorly differentiated tumors compared to G1 and G2 (p=0.046). MMP-9/TIMP-1 rate in serum was significantly higher in the study group than in the control group. The balance between the level of MMP-9 and TIMP-1 is disrupted in laryngeal cancer. The significant correlation between TIMP-1 expression and the presence of lymph node metastases, as well as that between TIMP-1 plasma concentration and stage of cancer histological differentiation, might indicate the importance of this molecule as a prognostic factor during carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression and Concentration of Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinases 1 in Laryngeal Squamous Cell Carcinoma

et al. 2019

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“…To our knowledge, there are few papers in the literature on the MMP and TIMP expression levels in the sera of patients with LSCC [23, 24, 26, 29].…”

Section: Discussionmentioning

confidence: 99%

Matrix metalloproteinases, inhibitor of metalloproteinases mRNA and protein expression in laryngeal squamous cell carcinoma

Grzelczyk¹,

Wróbel-Roztropiński²,

Szemraj³

et al. 2019

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Introduction The aim of the study was to investigate the mRNA expression and protein levels of matrix metalloproteinases (MMP) 2 (MMP-2), 9 (MMP-9), 7 (MMP-7) and their tissue inhibitor TIMP-2 in patients with laryngeal squamous cell carcinoma and control subjects and additionally to evaluate a possible correlation with clinicopathological features. Material and methods The expression levels of MMP-2, MMP-9, MMP-7, and TIMP-2 mRNA were detected by the real-time quantitative real time polymerase chain reaction method in 96 cases of laryngeal carcinoma vs. non-tumor tissue. The blood serum levels of MMP-2, MMP-9, MMP-7, and TIMP-2 in patients with laryngeal cancer and 100 healthy subjects were measured using the enzyme-linked immunosorbent assay (ELISA) method. Results The present study demonstrated that MMP-2, MMP-7, MMP-9 and TIMP-2 mRNA expression levels in carcinoma tissue vs. non-tumor tissue and protein levels in the preoperative serum vs. those obtained in healthy controls were statistically significantly higher than in the healthy controls ( p = 0.001). The only significant correlation between mRNA or concentration of measured MMPs and TIMP and the clinicopathological features was found for TIMP-2 protein and for patients with lymph node metastasis. Serum levels of TIMP-2 were higher in cases with lymph node metastasis than in those without lymph node metastasis ( p < 0.05). Conclusions The results may suggest that MMPs and TIMP-2 are associated with laryngeal tumorigenesis, but we did not find any distinct correlation between the clinicopathological features of laryngeal squamous cell carcinoma patients and expression levels of MMPs and TIMP. The results suggest that the measurement of serum MMP-2, MMP-7, MMP-9 and TIMP-2 concentration might be helpful to diagnose laryngeal squamous cell carcinoma.

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The levels of metalloproteineses and their endogenic inhibitors in patients with laryngeal cancer

Cited by 2 publications

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Expression and Concentration of Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinases 1 in Laryngeal Squamous Cell Carcinoma

Expression and Concentration of Matrix Metalloproteinase 9 and Tissue Inhibitor of Matrix Metalloproteinases 1 in Laryngeal Squamous Cell Carcinoma

Matrix metalloproteinases, inhibitor of metalloproteinases mRNA and protein expression in laryngeal squamous cell carcinoma

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