The Library of Human Miniantibodies in the Phage Display Format: Designing and Testing

Улитин, А. Б.; Kapralova, M. V.; Ламан, А. Г.; Shepelyakovskaya, A. O.; Bulgakova, Elena; Fursova, Ksenia; Abbasova, S. G.; Volkov, S. K.; Бровко, Ф. А.; Nesmeyanov, V. A.

doi:10.1007/s10628-005-0134-3

Cited by 8 publications

(3 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…cDNA is then synthesized on the base of isolated mRNA, and both oligo-dT and statistically devised hexanucleotides and specific primers can be used that yield cDNA copies of all the possible variable domain genes [29]. Also, one or several primers can be used to limit the range of amplified genes to one or several variable domain gene families or antibody isotypes [30].…”

Section: Library Constructionmentioning

confidence: 99%

Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection

Tikunova

Morozova

2009

Acta Naturae

View full text Add to dashboard Cite

The display of peptides and proteins on the surface of filamentous bacteriophage is a powerful methodology for selection of peptides and protein domains, including antibodies. An advantage of this methodology is the direct physical link between the phenotype and the genotype, as an analyzed polypeptide and its encoding DNA fragment exist in one phage particle. Development of phage display antibody libraries provides repertoires of phage particles exposing antibody fragments of great diversity. The biopanning procedure facilitates selection of antibodies with high affinity and specificity for almost any target. This review is an introduction to phage display methodology. It presents recombinant antibodies display in more details:, construction of phage libraries of antibody fragments and different strategies for the biopanning procedure.

show abstract

Section: Library Constructionmentioning

confidence: 99%

Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection

Tikunova

Morozova

2009

Acta Naturae

View full text Add to dashboard Cite

show abstract

“…Genes, encoding scFv against pesticin, were isolated by affinity selection on antigen technique using previously created antibody phage-display library [27,28].…”

Section: Cloning Of Scfv Genesmentioning

confidence: 99%

Refolding of scFv mini-antibodies using size-exclusion chromatography via arginine solution layer

Fursova

Ламан

Melnik

et al. 2009

Journal of Chromatography B

View full text Add to dashboard Cite

“…cDNA is then synthesized on the base of isolated mRNA, and both oligo-dT and statistically devised hexanucleotides and specific primers can be used that yield cDNA copies of all the possible variable domain genes [ 29 ]. Also, one or several primers can be used to limit the range of amplified genes to one or several variable domain gene families or antibody isotypes [ 30 ].…”

Section: Library Constructionmentioning

confidence: 99%

Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection

Tikunova

Morozova

2009

Acta Naturae

View full text Add to dashboard Cite

the display of peptides and proteins on the surface of filamentous bacteriophage is a powerful methodology for selection of peptides and protein domains, including antibodies. An advantage of this methodology is the direct physical link between the phenotype and the genotype, as an analyzed polypeptide and its encoding DnA fragment exist in one phage particle. Development of phage display antibody libraries provides repertoires of phage particles exposing antibody fragments of great diversity. the biopanning procedure facilitates selection of antibodies with high affinity and specificity for almost any target. this review is an introduction to phage display methodology. It presents recombinant antibodies display in more details:, construction of phage libraries of antibody fragments and different strategies for the biopanning procedure.

show abstract

The Library of Human Miniantibodies in the Phage Display Format: Designing and Testing

Cited by 8 publications

References 15 publications

Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection

Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection

Refolding of scFv mini-antibodies using size-exclusion chromatography via arginine solution layer

Phage Display on the Base of Filamentous Bacteriophages: Application for Recombinant Antibodies Selection

Contact Info

Product

Resources

About