The lignin fraction of animal feeding‐stuffs. I.—preliminary examination of lignin determination procedures

Moon, F. E.; Abou-raya, A. K.

doi:10.1002/jsfa.2740030905

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…This seems to be the explanation of the deviations shown by replicate lignin determinations using methods entailing ethanol washing, and in the preliminary experiments (Moon & Abou-Raya, 1952), when a homogeneous pre-treated and ethanol-washed starting material was used, the deviations were negligible. Heating the ethanol filtrate gave no precipitate, but dilution with water produced turbidity.…”

Section: The Alcohol-soluble Fraction Of Pre -Treated Materialsmentioning

confidence: 81%

The lignin fraction of animal feeding‐stuffs. II—Investigation of lignin determination procedures and development of a method for ‘acid‐insoluble lignin’

Moon¹,

Abou-raya²

1952

J Sci Food Agric

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Examination of humin residues obtained a t various stages during lignin determination and of the alcohol-soluble fraction of pre-treated food materials has indicated the necessity for modifying the procedures used in lignin determination. Factors affecting the dispersion and hydrolysis of cellulose are reported, and it is shown that in order t o determine as lignin only material insoluble in concentrated acid it is necessary t o filter without dilution after the main treatment. A method for determining ' acid-insoluble lignin ' is proposed and shown t o give satishctory agreement between replicates.* Throughout this paper the term ' concentrated hydrochloric acid ' refers to the ordinary laboratory reagent (containing about 3696 HCl), whereas ' fuming hydrochloric acid ' is a more concentrated product (containing 41-4396 HC1) prepared by treating the ordinary reagent with hydrogen chloride.

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Section: The Alcohol-soluble Fraction Of Pre -Treated Materialsmentioning

confidence: 81%

The lignin fraction of animal feeding‐stuffs. II—Investigation of lignin determination procedures and development of a method for ‘acid‐insoluble lignin’

Moon¹,

Abou-raya²

1952

J Sci Food Agric

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“…Many methods for the determination of lignin have been suggested but all are empirical and fail to isolate pure lignin (5,8). However, for pasture evaluation the requirement of any laboratory technique is that it should enable digestibility to be predicted with the greatest accuracy.…”

Section: Discussionmentioning

confidence: 99%

“…Of the chemical methods available, the lignin method has generally been found to predict digestibility with the lowest error (6). Most methods of analysing for lignin used in nutrition studies are empirical (5,8) and are based upon hydrolysis with 72% sulphuric acid. This method was originally developed for the analysis of wood pulp by Flechsig in 1883 (2).…”

Section: Introductionmentioning

confidence: 99%

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The Error in Predicting Pasture Dry–matter Digestibility From Four Different Methods of Analysis for Lignin

McLeod¹,

Minson²

1971

Grass and Forage Science

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The lignin content of 50 samples of five grasses of known in vivo digestibility were determined by the methods of Armitage, van Soest and two modifications of the van Soest technique. The error in predicting DM digestibility varied from ±31 for tbe Armitage metbod to ±5-0 for tbe van Soest method. This error compares nnfavourably witb ±21 previonsly obtained on tbe same samples with the in vitro technique of Tilley and Terry.

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The influence of the method of determination of lignin on the lignin‐ratio technique for digestibility in the cow

Balch

Rowland

1954

J Sci Food Agric

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Three methods for the determination of lignin (Norman & Jenkins, Ellis et al. and Armitage et al.) have been compared on samples of food and faeces from eight digestibility trials with lactating cows receiving a diet of hay and concentrates. Details are given of the different values obtained for crude lignin, the nitrogen content of the crude lignin, and lignin corrected for its nitrogen content. The method of Armitage et al. gave the lowest values. The mean digestibility coefficients for crude lignin and corrected lignin respectively, for the three methods in the above‐mentioned order, were 9.3 ± 0.7 and 8.3 ± 0.5, 4.7 ± 0.6 and 9.1 ± 0.7, 1.3 ± 0.8 and 4.4 ± 1.0. Thus lignin as determined by the method of Armitage et al., and expressed as crude lignin, showed a negligible digestibility, which should be acceptable as the basis of a ratio technique for the dairy cow. In a subsequent series of ten digestibility trials with cows on hay‐concentrate diets, and when using this method, the mean digestibility coefficient for crude lignin was ‐ 0.72 ± 0.7.

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The lignin fraction of animal feeding‐stuffs. I.—preliminary examination of lignin determination procedures

Cited by 10 publications

References 22 publications

The lignin fraction of animal feeding‐stuffs. II—Investigation of lignin determination procedures and development of a method for ‘acid‐insoluble lignin’

The lignin fraction of animal feeding‐stuffs. II—Investigation of lignin determination procedures and development of a method for ‘acid‐insoluble lignin’

The Error in Predicting Pasture Dry–matter Digestibility From Four Different Methods of Analysis for Lignin

The influence of the method of determination of lignin on the lignin‐ratio technique for digestibility in the cow

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