The linear 20 kb mitochondrial genome of Pandorina morum (Volvocaceae, Chlorophyta)

Moore, Linda J.; Coleman, Annette W.

doi:10.1007/bf00015557

Cited by 43 publications

(20 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, m t D N A of Chlamydomonas reinhardtii (Volvocales) is much smaller (15 kb [36]) and linear. Similar results were obtained for another species of the Vovocales, Pandorina morum, which was shown to possess a linear mitochondrial genome of about 20 kb [33]. For the chromophyte Olisthodiscus luteus a mitochondrial genome of 50 kb with circular shape has been demonstrated [ 1 ].…”

Section: Discussionsupporting

confidence: 72%

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina

Maerz¹,

Sitte²

1991

Plant Mol Biol

View full text Add to dashboard Cite

Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence of Hoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated from restriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I and Sal I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I and II, and apocytochrome b were localized on this map using Southern blot hybridization with heterologous gene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P. salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophytic algae, should be valuable for taxonomic and phylogenetic studies.

show abstract

Section: Discussionsupporting

confidence: 72%

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina

Maerz¹,

Sitte²

1991

Plant Mol Biol

View full text Add to dashboard Cite

show abstract

“…Moreover, in addition to C. reinhardtii , other Reinhardtinia algae are now known to have linear mitochondrial genomes (fig. 1 A ), such as Chlamydomonas asymmetrica (Laflamme and Lee 2003), the colonial volvocine Pandorina morum (Moore and Coleman 1989), and all known members of the nonphotosynthetic genus Polytomella (Smith, Hua, et al. 2013).…”

Section: Introductionmentioning

confidence: 99%

Multiple Independent Changes in Mitochondrial Genome Conformation in Chlamydomonadalean Algae

Hamaji

Kawai-Toyooka

Toyoda

et al. 2017

Genome Biology and Evolution

View full text Add to dashboard Cite

Chlamydomonadalean green algae are no stranger to linear mitochondrial genomes, particularly members of the Reinhardtinia clade. At least nine different Reinhardtinia species are known to have linear mitochondrial DNAs (mtDNAs), including the model species Chlamydomonas reinhardtii. Thus, it is no surprise that some have suggested that the most recent common ancestor of the Reinhardtinia clade had a linear mtDNA. But the recent uncovering of circular-mapping mtDNAs in a range of Reinhardtinia algae, such as Volvox carteri and Tetrabaena socialis, has shed doubt on this hypothesis. Here, we explore mtDNA sequence and structure within the colonial Reinhardtinia algae Yamagishiella unicocca and Eudorina sp. NIES-3984, which occupy phylogenetically intermediate positions between species with opposing mtDNA mapping structures. Sequencing and gel electrophoresis data indicate that Y. unicocca has a linear monomeric mitochondrial genome with long (3 kb) palindromic telomeres. Conversely, the mtDNA of Eudorina sp., despite having an identical gene order to that of Y. unicocca, assembled as a circular-mapping molecule. Restriction digests of Eudorina sp. mtDNA supported its circular map, but also revealed a linear monomeric form with a matching architecture and gene order to the Y. unicocca mtDNA. Based on these data, we suggest that there have been at least three separate shifts in mtDNA conformation in the Reinhardtinia, and that the common ancestor of this clade had a linear monomeric mitochondrial genome with palindromic telomeres.

show abstract

“…A linear mt genome flanked by inverted repeats has also been reported for the green alga Pandorina morum (Moore and Coleman 1989). In a phylogenetic analysis based on molecular, i.e., nuclear-encoded SSU and LSU rRNA sequences, and organismal data, this multicellular species turned out to be more closely related to C. reinhardtii and C. smithii than to C. eugametos and C. moewusii (Buchheim et al 1994).…”

Section: Discussionmentioning

confidence: 99%

The Mitochondrial Genome of Chlorogonium elongatum Inferred from the Complete Sequence

Kroymann

Zetsche

1998

J Mol Evol

View full text Add to dashboard Cite

The 22,704-bp circular mitochondrial DNA (mtDNA) of the chlamydomonad alga Chlorogonium elongatum was completely cloned and sequenced. The genome encodes seven proteins of the respiratory electron transport chain, subunit 1 of the cytochrome oxidase complex (cox1), apocytochrome b (cob), five subunits of the NADH dehydrogenase complex (nad1, nad2, nad4, nad5, and nad6), a set of three tRNAs (Q, W, M), and the large (LSU)- and small (SSU)-subunit ribosomal RNAs. Six group-I introns were found, two each in the cox1, cob, and nad5 genes. In each intron an open reading frame (ORF) related to maturases or endonucleases was identified. Both the LSU and the SSU rRNA genes are split into fragments intermingled with each other and with other genes. Although the average A + T content is 62.2%, GC-rich clusters were detected in intergenic regions, in variable domains of the rRNA genes, and in introns and intron-encoded ORFs. A comparison of the genome maps reveals that C. elongatum and Chlamydomonas eugametos mtDNAs are more closely related to one another than either is to Chlamydomonas reinhardtii mtDNA.

show abstract

The linear 20 kb mitochondrial genome of Pandorina morum (Volvocaceae, Chlorophyta)

Cited by 43 publications

References 22 publications

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina

Isolation, physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina

Multiple Independent Changes in Mitochondrial Genome Conformation in Chlamydomonadalean Algae

The Mitochondrial Genome of Chlorogonium elongatum Inferred from the Complete Sequence

Contact Info

Product

Resources

About