2018
DOI: 10.1074/jbc.m117.810184
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The linker histone H1.2 is a novel component of the nucleolar organizer regions

Abstract: The nucleoli accumulate rRNA genes (rDNA) and are the sites of rRNA synthesis and rRNA assembly into ribosomes. During mitosis, nucleoli dissociate but nucleolar remnants remain on the rDNA loci, forming distinct nucleolar organizer regions (NORs). Little is known about the composition and structure of NORs, but upstream binding factor (UBF) has been established as its master organizer. In this study, we sought to establish new proteins in NORs. Using UBF-Sepharose to isolate UBFbinding proteins, we identified… Show more

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Cited by 9 publications
(7 citation statements)
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“…3; Terme et al 2011). Although H1x is expressed in a DNA replication-independent manner (Happel et al 2005), its nuclear distribution has been shown to be cell cycle-dependent: During G1 phase, H1x is concentrated in the nucleoli, but upon entry into S-phase H1x is evenly dispersed in the nucleus until the next G1 phase (Stoldt et al 2007;Chen et al 2018). The purpose of this nucleolar localization in G1 remains unclear.…”
Section: H1xmentioning
confidence: 99%
See 1 more Smart Citation
“…3; Terme et al 2011). Although H1x is expressed in a DNA replication-independent manner (Happel et al 2005), its nuclear distribution has been shown to be cell cycle-dependent: During G1 phase, H1x is concentrated in the nucleoli, but upon entry into S-phase H1x is evenly dispersed in the nucleus until the next G1 phase (Stoldt et al 2007;Chen et al 2018). The purpose of this nucleolar localization in G1 remains unclear.…”
Section: H1xmentioning
confidence: 99%
“…H1.2 is the most well-studied somatic variant, but our understanding of its function is not straightforward. First, it is debatable as to whether H1.2 is an activator or repressor of transcription as both such activities have been reported (Brown et al 1996;Kim et al 2008Kim et al , 2013Kim et al , 2015Sancho et al 2008;Zheng et al 2010;Cao et al 2013;Izzo et al 2013;Millán-Ariño et al 2014;Mayor et al 2015;Chen et al 2018). Second, multiple unique biological functions of H1.2 have been proposed.…”
Section: H12mentioning
confidence: 99%
“…Curiously, phosphorylation of histone H1.2 at S173 increases during the M phase relative to the S phase, suggesting that this event is cell cycle-dependent and may serve as a marker for proliferation of cancer cells during BC invasion 23, 24 . Also, histone H1.2 is a novel component of the nucleolar organizer regions during mitosis 25 and H1.2 depletion was observed in a human BC cell line caused cell cycle G1-phase arrest 26 . Indeed, a higher mitotic index (≥ 7) in primary tumors is significantly associated with LN involvement 27 and higher mitotic indices accurately predict axillary LN involvement at operation 28 .…”
Section: Resultsmentioning
confidence: 99%
“…Different H1 variants interact with silencing factors in the nucleolus, such as macroH2A, heterochromatin proteins 1α (HP1α), HP1β, and HP1γ and DNMT3a/b, but also with active factors, such as UBF, nucleolin and topoisomerase I [ 84 , 85 , 86 ]. Histone H1.2 interacts with UBF in mitotic NORs [ 87 ]. The binding of H1 variants also affect the rRNA gene transcription, where deletion of UBF [ 32 ] and Actinomycin D [ 88 ] increase H1 and HP1 association with the rRNA gene repeat.…”
Section: The Inactive 47s Rrna Gene Repeats Are Dna Methylated and Organised In Constitutive Heterochromatinmentioning
confidence: 99%
“…Phosphorylated histone H1.2 and in particular histone H1.4 associate with active 47S rRNA genes repeats and rRNA inhibition reduces the phosphorylation level. Histone H1 interacts with the histone chaperones nucleolin, NPM, subunits of FACT as well as the heterochromatic proteins macroH2A, and HP1 and the active proteins UBF, histone H2AZ, histone H3.3 and DNA topoisomerase II [ 84 , 86 , 87 ]. The interaction of histone H1 with several histone chaperones indicates that one function of the chaperones is to regulate histone H1 and UBF and their PMT at the promoter.…”
Section: The Switch Between Active and Dna-methylated Inactive 47s Rrna Gene Repeatsmentioning
confidence: 99%