The Lipase of Pseudomonas fragi II. Factors Affecting Lipase Production

Nashif, S.A.; Nelson, F. E.

doi:10.3168/jds.s0022-0302(53)91523-9

Cited by 72 publications

(35 citation statements)

References 2 publications

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“…Nashif and Nelson (11) found more lipase activity in flask cultures of P. fragi than in cultures in test tubes; the differences in lipolytic activity, however, were less than the differences in total counts. Table 3 in this investigation the surface-volume ratio affects lipase production in some manner other than merely an effect on total count.…”

Section: Resultsmentioning

confidence: 92%

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LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARD^a

Alford

Elliott

1960

Journal of Food Science

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Section: Resultsmentioning

confidence: 92%

“…In the above microbial investigations in which the effect of temperature was studied (5,11), the substrates were coconut oil and butterfat. Both of these lipids contain appreciable amounts of the short-chained fatty acids.…”

mentioning

confidence: 99%

LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARD^a

Alford

Elliott

1960

Journal of Food Science

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“…The samples assayed contained 1 mg of ATF protein per ml. DISCUSSION P. fluorescens is a saprophytic bacterium (27) occurring in soil and commonly found as a contaminant of water, milk, and other refrigerated dairy foods (16)(17)(18)23). It is psychrophilic and grows best at room temperature.…”

Section: Resultsmentioning

confidence: 99%

“…For example, lysis of Staphylococcus aureus was produced by both P. aeruginosa and P. fluorescens (28). A protease was characterized from strain 26 of P. fluorescens, and lipase activity was exhibited by both P. fluorescens and P. fragi (16)(17)(18)22). However, these substances appear to be different from those reported in this study, e.g., in regard to molecular weight and resistance to heat and freezing.…”

Section: Resultsmentioning

confidence: 99%

Lysis of Trypanosoma cruzi by Pseudomonas fluorescens

Mercado

Colón-Whitt

1982

Antimicrob Agents Chemother

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Trypomastigotes of Trypanosoma cruzi isolated from the blood of infected mice were lysed within 24 h by an extracellular substance produced by Pseudomonas fluorescens. Isolation of the anti-trypanosomal factor (ATF) was accomplished by growth of the organisms in a defined medium, extracellular secretion by the sedimented cells, sterilization by filtration, lyophilization, dialysis, and gel filtration. Chromatographic separation with Sephadex G-25 and G-200 disclosed the occurrence of three active fractions. ATF-I(1) exhibited a molecular weight higher than 440,000. ATF-II and ATF-III were considerably smaller (molecular weights approximately 1,355 and 1,060, respectively). The lytic substance contained protein and lipopolysaccharide, was resistant to heat and freezing, was not proteolytic or hemolytic, and was not inhibited by trypsin but was suppressed by pronase.During studies of Trypanosoma cruzi, we observed that the number of trypomastigotes isolated from the blood of infected mice markedly diminished when maintained in vitro at 4°C for 3 days. Microscopic examination revealed that the parasite samples had become contaminated by a small, motile, rod-shaped microorganism that was attracted to the parasites, particularly to the posterior end. As a result of the interaction, the trypomastigotes became immobilized, and most were lysed within 24 h. A gram-negative bacterium isolated in blood agar and nutrient broth cultures was identified as Pseudomonas fluorescens by the Clinical Pathology Department, National Institutes of Health. We considered these observations to be significant not only in terms of cellular biology and physiology, but also because of their potential significance in the chemotherapy of Chagas' disease; further studies were therefore conducted to analyze the parasite-bacterium interaction.MATERIALS AND METHODS Parasites. The Tulahuen strain of T. cruzi is routinely maintained in this laboratory by blood passaging every 6 days in NIH-Swiss 16-to 20-g male mice; 4 x 106 to 5 x 106 trypomastigotes are usually inoculated intraperitoneally (14). The infection becomes acute after 6 days, producing marked pathology of liver and spleen, and blood counts of 108 parasites per ml are often obtained. The animals are not immunosuppressed. Blood samples are obtained under ether anesthesia directly from the heart after exposure of the cardiac surface. Parasites were isolated from blood by a two-step procedure (15) involving separation of the erythrocytes with Lymphoprep (9.6% sodium-Nmethyl-3,5-diacetamido-2,4,6-triiodobenzoate and 5.5% Ficoll; specific gravity, 1.077; Nyegaard and Co., Oslo, Norway) and elution through a DEAEcellulose column to remove lymphocytes and platelets. The eluted flagellates exhibited the usual lanceolate shape (6), were actively motile and elicited typical infections. Two myotropic strains of T. cruzi, the House 510 and the House 11, of Costa Rican and Nicaraguan origin, respectively, trypomastigotes of T. rhodesiense 1886, T. equiperdum, and promastigotes of Leishmania brasilien...

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“…(Rathi et al, 2001). However, maximum activity at higher pH (>7.0) has been observed in many cases (Nashif and Nelson 1953;Wang et al, 1995;Khyami-Horani 1996;Dong et al, 1999). The optimum temperature for lipase production corresponds with the growth temperature of the respective microorganism.…”

Section: Parameters For Optimum Lipase Productionmentioning

confidence: 99%

Isolation and Characterization of Lipase producing Bacteria from Vegetable Oil Spillage Site

Patel¹,

Mistry²,

Desai³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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The Lipase of Pseudomonas fragi II. Factors Affecting Lipase Production

Cited by 72 publications

References 2 publications

LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARD^a

LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARD^a

Lysis of Trypanosoma cruzi by Pseudomonas fluorescens

Isolation and Characterization of Lipase producing Bacteria from Vegetable Oil Spillage Site

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The Lipase of Pseudomonas fragi II. Factors Affecting Lipase Production

Cited by 72 publications

References 2 publications

LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARDa

LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARDa

Lysis of Trypanosoma cruzi by Pseudomonas fluorescens

Isolation and Characterization of Lipase producing Bacteria from Vegetable Oil Spillage Site

Contact Info

Product

Resources

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LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARD^a

LIPOLYTIC ACTIVITY OF MICROORGANISMS AT LOW AND INTERMEDIATE TEMPERATURES. I. ACTION OF PSEUDOMONAS FLUORESCENS ON LARD^a