The lipid composition of a halotolerant species of staphylococcus epidermidis

Komaratat, Prayad; Kates, M.

doi:10.1016/0005-2760(75)90197-6

Cited by 70 publications

(34 citation statements)

References 34 publications

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“…The fatty acid aiC15∶0 has been studied extensively and its presence in the plasma membrane of S. aureus has been proposed as the major determinant of fluidity [34].. Increasing NaCl concentration has previously been shown to result in increased plasma membrane levels of aiC15∶0 at salt levels of 15% w/w [35]. As this fatty acid is the dominant anteiso- fatty acid present in the plasma membrane, it is possible that it was required for alkaline stress adaption in addition to salt stress.…”

Section: Discussionmentioning

confidence: 99%

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

et al. 2014

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Staphylococcus lugdunensis has emerged as a major cause of community-acquired and nosocomial infections. This bacterium can rapidly adapt to changing environmental conditions to survive and capitalize on opportunities to colonize and infect through wound surfaces. It was proposed that S. lugdunensis would have underlying alterations in metabolic homeostasis to provide the necessary levels of adaptive protection. The aims of this project were to examine the impacts of subtle variations in environmental conditions on growth characteristics, cell size and membrane fatty acid composition in S. lugdunensis. Liquid broth cultures of S. lugdunensis were grown under varying combinations of pH (6–8), temperature (35–39°C) and osmotic pressure (0–5% sodium chloride w/w) to reflect potential ranges of conditions encountered during transition from skin surfaces to invasion of wound sites. The cells were harvested at the mid-exponential phase of growth and assessed for antibiotic minimal inhibitory concentration (MIC), generation time, formation of small colony variants, cell size (by scanning electron microscopy) and membrane fatty acid composition. Stress regimes with elevated NaCl concentrations resulted in significantly higher antibiotic resistance (MIC) and three of the combinations with 5% NaCl had increased generation times (P<0.05). It was found that all ten experimental growth regimes, including the control and centroid cultures, yielded significantly different profiles of plasma membrane fatty acid composition (P<0.0001). Alterations in cell size (P<0.01) were also observed under the range of conditions with the most substantial reduction occurring when cells were grown at 39°C, pH 8 (514±52 nm, mean ± Standard Deviation) compared with cells grown under control conditions at 37°C with pH 7 (702±76 nm, P<0.01). It was concluded that S. lugdunensis responded to slight changes in environmental conditions by altering plasma membrane fatty acid composition, growth rates and morphology to achieve optimal adaptations for survival in changing environments.

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Section: Discussionmentioning

confidence: 99%

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

et al. 2014

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“…By contrast, an increase of the ai15:0 in hyperosmotic medium has been reported for some Gram-positive halotolerant bacteria such as Planococcus sp. [26], Staphylococcus epidermidis [27] and Staphylococcus aureus [23]. The increase of ai17:0 amount in both strains in hyperosmotic medium suggests that these membranes may be less £uid when compared to membranes from control conditions grown cells.…”

Section: Discussionmentioning

confidence: 99%

Different cellular fatty acid pattern behaviours of two strains of Listeria monocytogenes Scott A and CNL 895807 under different temperature and salinity conditions

Chihib

2003

FEMS Microbiology Letters

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Cells of two strains of Listeria monocytogenes CNL 895807 and Scott A were grown to late exponential phase at different growth temperatures (37, 20 and 4 ‡C) with or without NaCl (7%), and their fatty acid compositions were analysed. The results showed that low thermal adaptation response of L. monocytogenes CNL was different than that of the Scott A strain, and it was based on both an increase of anteiso-branched-chain fatty acids and a significant decrease of straight-chain fatty acids. However, the main modifications observed in the Scott A strain when grown at a low temperature were a decrease of the proportion of ai17:0 and an increase of ai15:0. In hyperosmotic medium and over the entire temperature range (4 ‡C, 20 ‡C and 37 ‡C) the two L. monocytogenes strains showed a cellular fatty acid profile dominated by ai15:0. In addition, a decrease of the two major straight-chain fatty acids (14:0 and 16:0) was observed in the CNL strain. These results demonstrated that the CNL strain showed different behaviours of low thermal and salt adaptation to maintain membrane fluidity, which are based both on an increase of anteiso-branched-chain fatty acids, and a significant decrease of straight-chain fatty acids.

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“…It has been shown that V. costicola and some other moderate halophiles contain greater proportions of negatively charged phospholipids in their membranes when growing at higher salt concentrations (Komaratat & Kates, 1975;Ohno et al, 1979;Hanna et al, 1984). It has been suggested that this helps to counter the high Na+ concentration at the membrane surface (Hiramatsu et al, 1980).…”

Section: Introductionmentioning

confidence: 95%

Phospholipid Biosynthesis in the Moderately Halophilic Bacterium Vibvio costicola During Adaptation to Changing Salt Concentrations

Russell¹,

Kogut²,

Kates

1985

Microbiology

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The metabolism of phospholipids in Vibrio costicola, a moderately halophilic bacterium, has been investigated in relation to sudden changes in salinity. Both the absqlute and relative rates of biosynthesis of phosphatidylglycerol and phosphatidylethanolamine depend on the salt concentration of the medium; a sudden rise in salt concentration ha? an instantaneous inhibitory effect on phospholipid biosynthesis, but this inhibition lessens as, the bacteria adapt to the higher salinity. There is no turnover of phospholipids during isotonic growth, nor when the salt concentration is suddenly altered. The alterations in biosynthetic rates of phosphatidylglycerol and phosphatidylethanolamine that occur after sudden changes in salt concentration are consistent with the known compositional changes. We conclude that the mechanisms of changes in phospholipid composition during adaptation to raised or lowered salt concentrations are not necessarily the same.

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The lipid composition of a halotolerant species of staphylococcus epidermidis

Cited by 70 publications

References 34 publications

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

Small Changes in Environmental Parameters Lead to Alterations in Antibiotic Resistance, Cell Morphology and Membrane Fatty Acid Composition in Staphylococcus lugdunensis

Different cellular fatty acid pattern behaviours of two strains of Listeria monocytogenes Scott A and CNL 895807 under different temperature and salinity conditions

Phospholipid Biosynthesis in the Moderately Halophilic Bacterium Vibvio costicola During Adaptation to Changing Salt Concentrations

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