2019
DOI: 10.1093/femsyr/foz045
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The lipid droplet protein Pgc1 controls the subcellular distribution of phosphatidylglycerol

Abstract: The biosynthesis of yeast phosphatidylglycerol (PG) takes place in the inner mitochondrial membrane. Outside mitochondria, the abundance of PG is low. Here, we present evidence that the subcellular distribution of PG is maintained by the locally controlled enzymatic activity of the PG-specific phospholipase, Pgc1. A fluorescently labeled Pgc1 protein accumulates on the surface of lipid droplets (LD). We show, however, that LD are not only dispensable for Pgc1-mediated PG degradation, but do not even host any p… Show more

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Cited by 5 publications
(24 citation statements)
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“…Therefore, increased SE fraction in all the analyzed mutant strains, indicating a connection between PG and CL biosynthesis and lipid droplets, is not surprising. We showed before that Pgc1 localizes predominantly onto lipid droplets although the protein is active in membranes of endoplasmic reticulum and mitochondria (19). Increased lipid storage has been described as a frequent complication in TAZ1 deficient BTHS patients (12,41,42).…”
Section: Discussionmentioning
confidence: 85%
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“…Therefore, increased SE fraction in all the analyzed mutant strains, indicating a connection between PG and CL biosynthesis and lipid droplets, is not surprising. We showed before that Pgc1 localizes predominantly onto lipid droplets although the protein is active in membranes of endoplasmic reticulum and mitochondria (19). Increased lipid storage has been described as a frequent complication in TAZ1 deficient BTHS patients (12,41,42).…”
Section: Discussionmentioning
confidence: 85%
“…Intact mitochondria were isolated as described previously (17). The final mitochondrial pellet was suspended in the respiration buffer (0.6 M mannitol, 20 mM HEPES/KOH pH 7.1, 2 mM MgCl2, 1 mM EGTA, 0.1 % fatty acid-free bovine serum albumin, 10 mM KH2PO4) and used for measurement of O2 consumption, the activity of cytochrome c reductase, the activity of cytochrome c oxidase and ATP-hydrolase activity as described in (17) and measurement of Pgc1 activity as described in (19).…”
Section: Mitochondrial Enzymatic Assaysmentioning
confidence: 99%
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“…Several nutrient transporters of the yeast plasma membrane have been shown to accumulate in a specialized lateral microdomain, MCC (Membrane Compartment of arginine permease Can1) to prevent their ubiquitination and subsequent endocytosis in the absence of substrate [1][2][3]. Similarly, phosphatidylglycerol-specific phospholipase C, Pgc1, is activated and rapidly recognized by the endoplasmic reticulum-associated degradation (ERAD) pathway only after the release from the lipid droplet surface to the adjacent ER and/or outer mitochondrial membrane [4]. In stationary cells, cytosolic 5 -3 exoribonuclease Xrn1 is reversibly inactivated through binding at plasma membrane-associated, MCC-organizing protein complex, the eisosome [5,6].…”
Section: Introductionmentioning
confidence: 99%