The lipid fraction of human milk initiates adipocyte differentiation in 3T3-L1 cells

Fujisawa, Yasuko; Yamaguchi, Rie Shigetomi; Nagata, Eiko; Satake, Eiichiro; Sano, Saburo; Matsushita, Rie; Kitsuta, Kazunobu; Nakashima, S.; Nakanishi, Toshiki; Nakagawa, Yuichi; Ogata, Tsutomu

doi:10.1016/j.earlhumdev.2013.05.002

Cited by 7 publications

(9 citation statements)

References 43 publications

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“…The study did not reveal any difference in infant growth, weight gain and body fat at 12 months. Conversely, HM lipids affected preadipocyte differentiation in vitro in the absence of the standard adipogenic compounds, which was not observed with IF lipids (Fujisawa et al, 2013).…”

Section: 1mentioning

confidence: 89%

Towards infant formula biomimetic of human milk structure and digestive behaviour

Bourlieu-Lacanal

Deglaire

Oliveira

et al. 2017

OCL

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-Lipids of human milk or infant formula convey most of the energy necessary to support the newborn growth. Until recently, infant formula chemical composition had been optimized but not their structure. And yet, more and more proofs of evidence have shown that lipids structure in human milk modulates digestion kinetics and is involved in metabolic programming. Indeed there is a striking difference of structure between human milk which is an emulsion based on dispersed milk fat globules (4 mm) secreted by the mammary gland and submicronic neoformed lipid droplets (0.5 mm) found in infant formula. These droplets result from a series of operation units. This difference of structure modifies digestion kinetics and emulsion disintegration in the intestinal tract of the newborn. This difference persists along gastric phase which is mainly dominated by acid and enzyme-induced aggregation. Lipid droplets size is thus the key parameter to control gastric lipolysis and emptying and intestinal lipolysis. This parameter also controls proteolysis since adsorbed proteins are more rapidly hydrolyzed than when in solution. In animal models, these differences of lipid structure would also impact digestive and immune systems' maturation and microbiota. Lipid structure during neonatal period would also be involved in the early programming of adipose tissues and metabolism. The supplementation of infant formulas with bovine milk fractions (milk fat globule membrane extracts, triacylglycerol) or recent development of large droplets infant formula, along with new fields of innovation in neonatal nutrition, are here reviewed.Keywords: human milk fat globules / lipid structure / infant formula / neonatal digestion / programming Résumé -Vers des formules infantiles mimant la structure et le profil de digestion du lait maternel.Les lipides laitiers du lait maternel ou de formules infantiles fournissent la plupart de l'énergie disponible pour la croissance du nouveau-né. Jusqu'alors, la composition chimique des formules infantiles a été optimisée mais pas leur structure. Or il est de plus en plus démontré que la structure des lipides dans le lait maternel module les cinétiques de digestion et participe à la pré-programmation métabolique. Il existe en effet une différence majeure entre les émulsions natives de globules gras laitiers (4 mm) sécrétées par la glande mammaire et la structure des lipides dans les formules infantiles qui résultent d'une succession d'opérations unitaires de transformation et sont constituées de gouttelettes submicroniques (0.5 mm) avec des interfaces néoformées. Cette différence de structure modifie les cinétiques de digestion et de déstructuration des émulsions dans le tractus digestif des nouveau-nés. De façon générale, la différence de structure initiale entre lait maternel et formules infantiles se maintient pendant toute la phase gastrique, qui est surtout dominée par des mécanismes d'agrégation acide et enzymatique. La taille des gouttelettes est le paramètre clé pour contrôler les lipolyses gastri...

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Section: 1mentioning

confidence: 89%

Towards infant formula biomimetic of human milk structure and digestive behaviour

Bourlieu-Lacanal

Deglaire

Oliveira

et al. 2017

OCL

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“…Previous studies reported that milk strongly stimulates the differentiation of 3T3‐L1 preadipocytes, a cell model for adipocyte differentiation . In a standard differentiation program, growth‐arrested confluent 3T3‐L1 preadipocytes are treated with appropriate hormonal agents, which induce synchronous reentrance into the cell cycle and undergo at least 2 rounds of mitosis, referred to as mitotic clonal expansion.…”

Section: Discussionmentioning

confidence: 99%

“…During mitotic clonal expansion, the expression of the adipogenic transcription factors C/ebpβ and C/ebpδ is induced, which leads to the induction and activation of Pparγ, a master regulator of adipogenesis . A treatment with the lipid fraction of milk, even in the absence of appropriate hormonal reagents, was found to induce C/ebpβ expression during mitotic clonal expansion and increase lipid accumulation in 3T3‐L1 adipocytes . In addition, milk has been shown to significantly activate Pparγ as a transcription factor .…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies reported that milk strongly stimulates the differentiation of 3T3-L1 preadipocytes, a cell model for adipocyte differentiation. 13,14 In a standard differentiation program, growtharrested confluent 3T3-L1 preadipocytes are treated with appropriate hormonal agents, which induce synchronous reentrance into the cell cycle and undergo at least 2 rounds of mitosis, referred to as mitotic clonal expansion. During mitotic clonal expansion, the expression of the adipogenic transcription factors C/ebpβ and C/ebpδ is induced, which leads to the induction and activation of Pparγ, a master regulator of adipogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, human milk stimulated the differentiation of white preadipocytes. 13,14 In view of overlapping mechanisms between brown adipogenesis and white adipogenesis, 15 milk may also have the potential to modulate brown adipogenesis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Modulation of brown adipocyte activity by milk by‐products: Stimulation of brown adipogenesis by buttermilk

Asano

Kida

Muto

et al. 2016

Cell Biochemistry & Function

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Brown adipocytes dissipate chemical energy in the form of heat through the expression of mitochondrial uncoupling protein 1 (Ucp1); Ucp1 expression is further upregulated by the stimulation of β-adrenergic receptors in brown adipocytes. An increase in energy expenditure by activated brown adipocytes potentially contributes to the prevention of or therapeutics for obesity. The present study examined the effects of milk by-products, buttermilk and butter oil, on brown adipogenesis and the function of brown adipocytes. The treatment with buttermilk modulated brown adipogenesis, depending on the product tested; during brown adipogenesis, buttermilk 1 inhibited the differentiation of HB2 brown preadipocytes. In contrast, buttermilk 3 and 5 increased the expression of Ucp1 in the absence of isoproterenol (Iso), a β-adrenergic receptor agonist, suggesting the stimulation of brown adipogenesis. In addition, the Iso-induced expression of Ucp1 was enhanced by buttermilk 2 and 3. The treatment with buttermilk did not affect the basal or induced expression of Ucp1 by Iso in HB2 brown adipocytes, except for buttermilk 5, which increased the basal expression of Ucp1. Conversely, butter oil did not significantly affect the expression of Ucp1, irrespective of the cell phase of HB2 cells, ie, treatment during brown adipogenesis or of brown adipocytes. The results of the present study indicate that buttermilk is a regulator of brown adipogenesis and suggest its usefulness as a potential food material for antiobesity.

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Nutritional Significance of Milk Lipids: From Bioactive Fatty Acids to Supramolecular Structures Impacting Metabolism

Guillocheau

Milard

Rioux

et al. 2020

Advanced Dairy Chemistry, Volume 2

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The lipid fraction of human milk initiates adipocyte differentiation in 3T3-L1 cells

Cited by 7 publications

References 43 publications

Towards infant formula biomimetic of human milk structure and digestive behaviour

Towards infant formula biomimetic of human milk structure and digestive behaviour

Modulation of brown adipocyte activity by milk by‐products: Stimulation of brown adipogenesis by buttermilk

Nutritional Significance of Milk Lipids: From Bioactive Fatty Acids to Supramolecular Structures Impacting Metabolism

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