The lipid language of plant–fungal interactions

Christensen, Shawn A.; Kolomiets, Michael V.

doi:10.1016/j.fgb.2010.05.005

Cited by 186 publications

(160 citation statements)

References 103 publications

(121 reference statements)

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“…The functions of 9-LOX products are less well known. However, Christensen and Kolomiets (2011) suggest that 9-LOX derivatives have hormone-like activities and may regulate growth, development, and defense against pathogens and pests.…”

Section: Characterization Of Lox Reaction Productsmentioning

confidence: 99%

Purification and properties of lipoxygenase from opium poppy seedlings(Papaver somniferum L.)

Holková¹,

Bilka²,

Rauová³

et al. 2016

Turk J Biol

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Lipoxygenase (linoleate:oxygen oxidoreductase; EC 1.13.11.12; LOX) is a key enzyme in the signaling pathway leading to the production of biologically active compounds involved in plant growth development and stress responses. In this study the novel LOX enzyme from opium poppy (Papaver somniferum L.) seedlings was purified to electrophoretic homogeneity and characterized. LOX activity during the germination of opium poppy seeds was analyzed, and the highest LOX activity was determined on the fourth day of germination. Opium poppy LOX was purified from 4-day-old poppy seedlings after ammonium sulfate precipitation followed by hydrophobic chromatography (Phenyl-Sepharose CL-4B), ion exchange chromatography (Q-Sepharose), and affinity chromatography (linoleyl-aminopropyl agarose) for the first time. The relative molecular weight of purified LOX was estimated to be 78 kDa by immunoblotting. The highest enzyme activity occurred at pH 6.5 and 0.75 mM calcium ion concentration. LOX showed preferential activity towards linoleic acid followed by linolenic acid as a substrate. To investigate the positional specificity of the LOX reaction, purified LOX was incubated with linoleic acid, and the products were analyzed by high-performance liquid chromatography. Opium poppy LOX converted linoleic acid to 13-hydroperoxy-(9Z,11E)-octadecadienoic acid (63.5%) and, to a lesser extent, 9-hydroperoxy-(10E,12Z)-octadecadienoic acid (36.5%).

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Section: Characterization Of Lox Reaction Productsmentioning

confidence: 99%

Purification and properties of lipoxygenase from opium poppy seedlings(Papaver somniferum L.)

Holková¹,

Bilka²,

Rauová³

et al. 2016

Turk J Biol

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“…According to current dogma, plants express LOXs and generate oxylipins in defense reactions ( 3 ). LOXs of fungi may also form specifi c oxylipins and participate in this complex chemical warfare ( 12 ). This is probably one of the major functions of 9 S -LOX and EAS of M. salvinii .…”

Section: What Is the Biological Function Of 9 S-mnlox And Epoxy Alcohmentioning

confidence: 99%

“…This is probably one of the major functions of 9 S -LOX and EAS of M. salvinii . Fungal oxylipins can also participate in sporulation ( 12 ). M. salvinii forms black sclerotia with conidia in the necrotic rice stem.…”

Section: What Is the Biological Function Of 9 S-mnlox And Epoxy Alcohmentioning

confidence: 99%

Secretion of two novel enzymes, manganese 9S-lipoxygenase and epoxy alcohol synthase, by the rice pathogen Magnaporthe salvinii

Wennman

Oliw

2013

Journal of Lipid Research

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“…The synthesis of oxylipins is first catalyzed by lipoxygenases (LOXs), which add molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield the corresponding fatty acid hydroperoxides that are substrates for other enzymes (figure 4) [24]. Based on their regiospecificity, the dioxygenation occurs at C-9 or C-13 and LOXs have been thus classified as 9-and 13-LOX, which yield 9-or 13-hydroperoxides, respectively [25]. In the case of linolenic acid C18:3 and 13-LOX, the resulting product is 13-HPOT (hydroperoxy octadectrienoic acid) [15].…”

Section: Introductionmentioning

confidence: 99%

Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach

Tayeh¹,

Randoux²,

Laruelle³

et al. 2013

Lipid Metabolism

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The lipid language of plant–fungal interactions

Cited by 186 publications

References 103 publications

Purification and properties of lipoxygenase from opium poppy seedlings(Papaver somniferum L.)

Purification and properties of lipoxygenase from opium poppy seedlings(Papaver somniferum L.)

Secretion of two novel enzymes, manganese 9S-lipoxygenase and epoxy alcohol synthase, by the rice pathogen Magnaporthe salvinii

Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach

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