The lipids of fish. 2. The acetone-soluble lipids of the flesh of the haddock

Abstract

1. Haddock flesh contains a total of about 1 % lipid, but only about 0 1-0 2% of 'free' lipid, the rest being probably 'bound' to protein.2. Extraction with a series of solvents suggests the presence of more than one type of linkage between lipid and protein although the picture is complicated by probable differences in solubility of the various lipid constituents.

The lipids of fish. 5. The lipids remaining in the flesh of the haddock after extraction by acetone and ethanol-ether

“…refluxing with 6N methanolic HCI of another sample for determination of fatty acids and unsaponifiable matter. In contrast to ethanolic alkali and aqueous HCI (Lovern & Olley, 1953a;, methanolic HCI gave fatty acids (as methyl esters), entirely free from nitrogen.…”

Determination of Serum Cholesterol With Perchloric Acid

“…Using the method of Zlatkis et al (1953), Kinley and Krause (1958) have reported high estimates for determination of cholesterol in the presence of high concentration of vitamin A, and it seems highly unsaturated compounds generally can com-plicate accurate cholesterol determination. A number of substances may liave contributed to the errors found by Lovem and Olley (1953), but the question is confused by ignorance of the elution charactenstics on silicic acid of possible contaminants (e.g. other stcrol esters, wax esters, lipid vitamin esters) in fish flesh.…”

Review on fish phospholipids

“…An acetone extract of haddock flesh Gudus uegleJinus was separated, by Lovern et al [3], into three fractions comprising (A) acetone-soluble non-phosphatidic lipids plus some lecithin, (B) mainly lecithin soluble in acetone in the absence of added electrolytes, and (C) acetone-insoluble lipids. Fraction A consisted of about 32 % lecithin, 18 % free cholesterol, 18 % free fatty acids, 10 % cholesterol esters, 8 % waxes, 7 % triglycerides, 2 % hydrocarbons, etc.…”