The lipoxygenase sensor, a new approach in essential fatty acid determination in foods

Schoemaker, M.; Feldbrügge, Rainer; Gründig, Bernd; Spener, Friedrich

doi:10.1016/s0956-5663(97)00073-0

Cited by 25 publications

(11 citation statements)

References 30 publications

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“…The measured current was directly proportional to the concentration of triolein/TG. In 1997, Schoemaker et al constructed a sensitive three-electrode system for the determination of triglycerides [31]. Collagen membrane was used to immobilize diaphorase and GIDH enzymes.…”

Section: Membrane Based Tg Biosensorsmentioning

confidence: 99%

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

Hooda

Gahlaut

Gothwal

2018

Artificial Cells, Nanomedicine, and Biotechnology

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Clinical manifestations of the elevated plasma triacylglycerol (TG) include a greater prevalence of atherosclerotic heart disease, acute pancreatitis, diabetes mellitus, hypertension, and ischemic vascular disease. Hence, these significant health troubles have attracted scientific attention for the precise detection of TG in biological samples. Numerous techniques have been employed to quantify TG over many decades, but biosensors hold the leading position owing to their superior traits such as highly specific recognition for target molecules, accuracy, minituarization, small sample requirement and rapid response. Enzyme-based electrochemical biosensors represent an instantaneous resolution for the foremost bottlenecks constraining laboratory prototypes to reach real time bedside applications. We highlight the choice of transducers and constructive strategies to design high-performance biosensor for the quantification of triglycerides in sera and early diagnosis of health problems related to it. In the present review, a small effort has been made to emphasize the significant role of enzymes, nanostructured metal oxides, graphene, conducting polypyrrole, nanoparticles, porous silicon, EISCAP and ENFET in enabling TG biosensors more proficient and taking a revolutionary step forward.

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Section: Membrane Based Tg Biosensorsmentioning

confidence: 99%

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

Hooda

Gahlaut

Gothwal

2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…Schoemaker et al [114] used both an enzyme electrode (in a batch system) and an enzyme column (in a FIA manifold) with immobilized lipoxygenase for the determination of essential fatty acids, e.g., linoleic and linolenic acids. The oxygen depletion due to the action of the enzyme was monitored amperometrically.…”

Section: Essential Fatty Acidsmentioning

confidence: 99%

Enzyme Based Amperometric Biosensors for Food Analysis

2002

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“…The second oxygenation activities were carried out in phosphate buffer (pH 6.0, 0.2 M) by using ALA as a substrate (Schoemaker et al, 1997). All measurements were carried out using a thermostatic cell at 30°C.…”

Section: Working Conditionsmentioning

confidence: 99%

An Enzyme Electrode Based on Lipoxygenase Immobilized in Gelatin for Selective Determination of Essential Fatty Acids

Tımur

Önal

Akyılmaz

et al. 2003

Artificial Cells, Blood Substitutes, and Biotechnology

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An enzyme electrode for the specific determination of omega-3 and omega-6 fatty acids from the mixture of essential fatty acids (EFAs) was developed by using lipoxygenase (LOX) (EC 1.13.11.12) from soy beans in combination with a dissolved oxygen (DO) probe. The enzyme electrode showed different sensitivities for linoleic (LA) and alpha-linolenic acids (ALA), the most common essential fatty acids. Enzyme electrode response depends linearly on LA concentration between 12.8-160.5 microM and ALA concentration between 3.8-18.9 microM in borate buffer, 0.2 M at pH 9.0. However, in phosphate buffer 0.2 M at pH 6.0 linearity is in the range of 7.5-22.5 microM of ALA concentration at 5 minutes response times. Moreover, maximum electrode response was found in borate buffer at pH 9.0 and 30 degrees C.

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The lipoxygenase sensor, a new approach in essential fatty acid determination in foods

Cited by 25 publications

References 30 publications

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

Recent trends and perspectives in enzyme based biosensor development for the screening of triglycerides: a comprehensive review

Enzyme Based Amperometric Biosensors for Food Analysis

An Enzyme Electrode Based on Lipoxygenase Immobilized in Gelatin for Selective Determination of Essential Fatty Acids

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