Recently our study identified EP3 receptor and galectin-3 as prognosticators of cervical cancer. The aim of the present study was the analysis of EP2 as a novel marker and its association to EP3, galectin-3, clinical pathological parameters and the overall survival rate of cervical cancer patients. Cervical cancer tissues (n = 250), as also used in our previous study, were stained with anti-EP2 antibodies employing a standardized immunohistochemistry protocol. Staining results were analyzed by the iRS scores and evaluated for its association with clinical-pathological parameters. H-test of EP2 percent-score showed significantly different expression in FIGO I-IV stages and tumor stages. Kaplan-Meier survival analyses indicated that EP3-negative/EP2-high staining patients (EP2 IRS score ≥2) had a significantly higher survival rate than the EP3-negative/EP2-low staining cases (p = 0.049). In the subgroup of high galectin-3 expressing patients, the group with high EP2 levels (IRS ≥2) had significantly better survival rates compared to EP2-low expressing group (IRS <2, p = 0.044). We demonstrated that the EP2 receptor is a prognostic factor for the overall survival in the subgroup of negative EP3 and high galectin-3 expressed cervical cancer patients. EP2 in combination with EP3 or galectin-3 might act as prognostic indicators of cervical cancer. EP2, EP3, and galectin-3 could be targeted for clinical diagnosis or endocrine treatment in cervical cancer patients, which demands future investigations.Cervical cancer is the fourth most common female cancer type worldwide with nearly half a million new cases annually. 83% of all cases occur in developing countries, whereas in developed countries only 3.6% of new cancer cases are cervical cancer 1 . One of the main risk factors for cervical cancer is a persistent infection with specific Human Papillomavirus (HPV), the high-risk papillomavirus (HR-HPV) 2 . HPV is manifested in nearly 99.7% of all cervical cancer patients 3 . Belonging to the papillomavirus family, HPV is a non-enveloped, small, double-stranded DNA virus 3-5 . More than 200 HPV genotypes have been characterized worldwide 6 . The sub-classification in high-risk and low-risk is important for the HPV infections of the genital tract 5 . Low-risk subtypes, such as HPV-6, HPV-11, HPV-26, HPV-40, HPV-42, are the cause of genital warts and non-malignant lesions 2,5 , whereas high-risk HPV types like HPV-16 and HPV-18 were identified in cervical and other anogenital cancers 2,5 .Tumor cell differentiation, apoptosis, and oncogenesis are associated with prostanoids, including prostaglandin E 2 (PGE 2 ), prostaglandin D 2 (PGD 2 ), prostaglandin I 2 (PGI 2 ), prostaglandin F 2 (PGF 2 ) and thromboxane A 2 7 . Prostaglandins are important for tumor progression and tumor-associated angiogenesis as Amano et al. described 8 . PGE 2 signaling is well-known for apoptosis inhibition, angiogenesis, metastatic formation, and tumor progression. The membrane-bound EP receptors specific for PGE 2 are G-protein coupled receptors and...