The localization of calcitonin gene-related peptide in the human trigeminal ganglion and masseter muscle

Azuma, Yuri; Sato, Iwao

doi:10.2535/ofaj.93.127

Cited by 5 publications

(7 citation statements)

References 28 publications

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“…CGRP-positive neurons are notably prevalent within the TMJ complex, including areas like the joint capsule, synovial membrane, temporomandibular joint posterior disc tissue, masticatory muscles, tendons, and fascia (Azuma and Sato 2017 ; Lindquist et al 2021 ; Suttle et al 2023 ). Echoing our findings, CGRP was predominantly expressed around the blood vessels in the temporomandibular joint tissues of rats.…”

Section: Discussionmentioning

confidence: 99%

Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis

Tao,

Wang,

2024

Cell Mol Neurobiol

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Calcitonin gene-related peptide (CGRP) is synthesized and secreted by trigeminal ganglion neurons, and is a key neuropeptide involved in pain and immune regulation. This study investigates the expression of CGRP in the trigeminal ganglion (TG) and its regulatory role in the polarization of macrophages in rats with temporomandibular arthritis. A rat model of temporomandibular arthritis was established using CFA. Pain behavior was then observed. Temporomandibular joint (TMJ) and the TG were collected, and immunohistochemistry, immunofluorescence (IF) staining, and RT-qPCR were used to examine the expression of CGRP and macrophage-related factors. To investigate the impact of CGRP on macrophage polarization, both CGRP and its antagonist, CGRP 8-37, were separately administered directly within the TG. Statistical analysis revealed that within 24 h of inducing temporomandibular arthritis using CFA, there was a significant surge in CD86 positive macrophages within the ganglion. These macrophages peaked on the 7th day before beginning their decline. In this context, it’s noteworthy that administering CGRP to the trigeminal ganglion can prompt these macrophages to adopt the M2 phenotype. Intriguingly, this study demonstrates that injecting the CGRP receptor antagonist (CGRP 8-37) to the ganglion counteracts this shift towards the M2 phenotype. Supporting these in vivo observations, we found that in vitro, CGRP indeed fosters the M2-type polarization of macrophages. CGRP can facilitate the conversion of macrophages into the M2 phenotype. The phenotypic alterations of macrophages within the TG could be instrumental in initiating and further driving the progression of TMJ disorders.

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Section: Discussionmentioning

confidence: 99%

Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis

Tao,

Wang,

2024

Cell Mol Neurobiol

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“…The masseter muscle contains many tender points. 31 According to a recent study, CGRP-immunopositive reaction fibres were observed in the vascular wall of the fascia of the masseter muscle, 32 suggesting the involvement of the masseter muscle fascia in nociception. The masseter has many muscle bundles, and therefore, the surface area of its fascia is large.…”

Section: Ta B L Ementioning

confidence: 97%

“…In terms of the painful site, patients with pain only in the masseter muscle had a median change of 24 (IQR 8-36) in the level of pain, while those with pain in the masseter and other muscles had a median change of 14 (IQR 5-31); no significant difference was observed between the two. Patients under the care of the experienced doctor had a median change of[24][25][26][27][28][29][30][31][32][33][34][35] in the pain level, while those under the care of the inexperienced doctor had a median change of 14 (IQR 8-36),…”

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confidence: 99%

Immediate curative effects of exercise therapy in patients with myalgia of the masticatory muscles

Sakaguchi

Taguchi

Kobayashi³

et al. 2022

J of Oral Rehabilitation

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Background: Exercise therapy is occasionally considered as an initial treatment for temporomandibular disorders. However, pain can be exacerbated during exercise therapy.Objective: To investigate the immediate curative effects of exercise therapy in patients with masticatory muscle myalgia.Methods: Fifty-nine patients with masticatory muscle myalgia were included.Therapists performed exercise therapy (stretched the painful masseter and/or cervical muscles along the direction of muscle contraction) in 10 rounds of traction, each lasting 10 s. The patient's pain-free maximum mouth opening distance and degree of pain (VAS value) before and immediately after exercise therapy were compared using the Wilcoxon signed-rank test. The Mann-Whitney U test was used for the subgroup comparisons.Results: Mouth opening increased from 41 to 46 (IQR 43-48) mm and pain alleviation from 48 (IQR 31-56) to 21 (IQR 10-56) immediately following exercise therapy (p < .001 for both). None of the patients experienced pain exacerbation or reduction in mouth opening post-exercise. No difference in mouth opening distance changes according to sex, painful side, painful site and therapist were observed (p > .05 for all). Pain reduction was greater in patients with unilateral pain (26, IQR 12-39) than those with bilateral (13, IQR 5-25) (p = .019). There were no differences in the change in the degree of pain according to sex, painful site and therapist (p > .05 for all). Conclusion:Exercise therapy immediately enlarged the mouth opening distance and reduced myalgia; therefore, it could be helpful in managing masticatory muscle myalgia.

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“…CGRP-containing nerve endings are present in masseter muscle and in synovial membrane, articular disc, periosteum, and capsule of the temporomandibular joint (TMJ). [21][22][23] Elevated CGRP concentrations were reported in the TMJ synovium in the presence of TMJ internal derangements 24 and in the TMJ synovial fluid in TMJ osteoarthritis. 25,26 However, the CGRP level in blood of people with painful TMD has not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Plasma Calcitonin Gene-Related Peptide as a Biomarker for Painful Temporomandibular Disorder and Migraine

Tchivileva,

Johnson,

Chai

et al. 2023

JPR

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Objective To assess associations of plasma calcitonin gene-related peptide (CGRP) with chronic temporomandibular disorder (TMD) myalgia/arthralgia or frequent/chronic migraine, alone and in combination, and to evaluate relations between the CGRP concentration and clinical, psychological, and somatosensory characteristics of participants. Methods The cross-sectional study selected four groups of adult volunteers: healthy controls (HCs), TMD without migraine, migraine without TMD, and TMD with migraine. Each group comprised 20 participants, providing 94% power to detect statistically significant associations with CGRP concentration for either TMD or migraine. TMD and headache were classified according to the Diagnostic Criteria for TMD and the International Classification for Headache Disorders, 3rd edition, respectively. Plasma CGRP was quantified with a validated high-sensitivity electrochemiluminescent Meso Scale Discovery assay. Questionnaires and clinical examinations were used to evaluate characteristics of TMD, headache, psychological distress, and pressure pain sensitivity. Univariate regression models quantified associations of the CGRP concentration with TMD, migraine, and their interaction. Univariate associations of the CGRP concentration with clinical, psychological, and pressure pain characteristics were also assessed. Results Among 80 participants enrolled, neither TMD nor migraine was associated with plasma CGRP concentration ( P = 0.761 and P = 0.972, respectively). The CGRP concentration (mean ± SD) was similar in all 4 groups: HCs 2.0 ± 0.7 pg/mL, TMD 2.1 ± 0.8 pg/mL, migraine 2.1 ± 0.9 pg/mL, and TMD with migraine 2.2 ± 0.7 pg/mL. CGRP concentration was positively associated with age ( P = 0.034) and marginally with body mass index ( P = 0.080) but was unrelated to other participant characteristics. Conclusion In this well-powered study, interictal plasma concentration of CGRP was a poor biomarker for TMD and migraine.

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The localization of calcitonin gene-related peptide in the human trigeminal ganglion and masseter muscle

Cited by 5 publications

References 28 publications

Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis

Expression of CGRP in the Trigeminal Ganglion and Its Effect on the Polarization of Macrophages in Rats with Temporomandibular Arthritis

Immediate curative effects of exercise therapy in patients with myalgia of the masticatory muscles

Evaluation of Plasma Calcitonin Gene-Related Peptide as a Biomarker for Painful Temporomandibular Disorder and Migraine

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