The Localization of p53 in the Crayfish Mechanoreceptor Neurons and Its Role in Axotomy-Induced Death of Satellite Glial Cells Remote from the Axon Transection Site

Rodkin, Stanislav; Khaitin, Andrey; Pitinova, Maria; Dzreyan, Valentina; Guzenko, Valeria; Rudkovskii, M. V.; Sharifulina, Svetlana; Uzdensky, Anatoly B.

doi:10.1007/s12031-019-01453-2

Cited by 13 publications

(20 citation statements)

References 46 publications

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“…To study the neuroprotective effect of Hsp70, we used two models of axotomy in vitro: bilaterally axotomized ganglia of the crayfish A. leptodactylus ventral nerve cord (VNC) and isolated mechanoreceptor neuron (MRN) of the crayfish stretch receptor (CSR) [ 7 , 15 , 16 , 17 , 18 ]. The animal protocols were evaluated and approved by the Animal Care and Use Committee of the Southern Federal University (approval no.…”

Section: Methodsmentioning

confidence: 99%

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The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy

Demyanenko

Pitinova

Dzreyan

et al. 2021

Cells

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The search for effective neuroprotective agents for the treatment of neurotrauma has always been of great interest to researchers around the world. Extracellular heat shock protein 70 (eHsp70) is considered a promising agent to study, as it has been demonstrated to exert a significant neuroprotective activity against various neurodegenerative diseases. We showed that eHsp70 can penetrate neurons and glial cells when added to the incubation medium, and can accumulate in the nuclei of neurons and satellite glial cells after axotomy. eHsp70 reduces apoptosis and necrosis of the glial cells, but not the neurons. At the same time, co-localization of eHsp70 with p53 protein, one of the key regulators of apoptosis, was noted. eHsp70 reduces the level of the p53 protein apoptosis promoter both in glial cells and in the nuclei and cytoplasm of neurons, which indicates its neuroprotective effect. The ability of eHsp70 to reverse the proapoptotic effect of the p53 activator WR1065 may indicate its ability to regulate p53 activity or its proteosome-dependent degradation.

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Section: Methodsmentioning

confidence: 99%

“…Earlier, cell models of axotomy showed an increase in p53 protein levels in the nuclei of neurons and glial cells. P53 activators WR-1065 and nutlin-3 increased the apoptosis of glial cells in the mechanoreceptor neuron of the crayfish A. leptodactylus [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy

Demyanenko

Pitinova

Dzreyan

et al. 2021

Cells

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“…The slowly adapting neuron constantly generates electric spikes with frequency, proportional to the extension of the receptor muscle, while the other neurons has phasic activity in the form of a series of spikes in response to the extra stretching of the muscle. The spikes propagate to the ventral nerve cord ganglion [16][17][18] . The ultrastructure of this neuron was studied in detail in many works [19][20][21] .…”

Section: Methodsmentioning

confidence: 99%

“…The ultrastructure of this neuron was studied in detail in many works [19][20][21] . It proved itself a convenient model object for studying the response of neurons and glial cells to various damaging impacts including axotomy 17 .…”

Section: Methodsmentioning

confidence: 99%

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Expression and Localization of C-APP and N-APP in Peripheral Neurons of Rats and Crayfish After Axotomy

Rodkin¹,

Dzreyan²,

Khaitin³

et al. 2021

Preprint

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Nerve injury induces a cascade of molecular-cellular events, leading to neuronal death or survival, where amyloid precursor protein (APP) and its proteolytic products play an important role. We studied the localization and expression of C-APP and N-APP in rat dorsal root ganglia (DRG) with transected sciatic nerve, axotomized crayfish stretch receptor neuron (SRN) and ventral nerve cord (VNC) ganglia with transected connectives. C-APP and N-APP localized predominantly in neurons, not in glial cells. Axotomy increased C-APP and N-APP expression in rat and crayfish neurons. The expression of APP in crustaceans confirms its conservative nature. In DRG, C-APP level was higher in neuronal nuclei than in cytoplasm in 24 hours post-axotomy. N-APP accumulation was not observed in DRG and crayfish neuronal nuclei. SRN axotomy resulted in C-APP and N-APP accumulation in 4–8 hours in perikaryon and its extensions, but only С-APP accumulated in nuclei. This indicates that not the whole APP, but its C-terminal product, AICD, enters the nucleus. Also, there was high level of C-APP in SRN nucleolus, suggesting possible AICD involvement in rRNA synthesis and ribosome formation. The APP accumulation in transected axons confirms its involvement in injury-induced axonal events.

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Preventive Strategies for Chemotherapy-Induced Peripheral Neuropathy

Nielsen

Herrstedt

2021

Diagnosis, Management and Emerging Strategies for Chemotherapy-Induced Neuropathy

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The Localization of p53 in the Crayfish Mechanoreceptor Neurons and Its Role in Axotomy-Induced Death of Satellite Glial Cells Remote from the Axon Transection Site

Cited by 13 publications

References 46 publications

The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy

The Role of p53 Protein in the Realization of the Exogenous Heat Shock Protein 70 Anti-Apoptotic Effect during Axotomy

Expression and Localization of C-APP and N-APP in Peripheral Neurons of Rats and Crayfish After Axotomy

Preventive Strategies for Chemotherapy-Induced Peripheral Neuropathy

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