The location of chloride in single striated muscle fibers of the giant barnacle

Gayton, D. C.; Hinke, J. A. M.

doi:10.1139/y68-035

Cited by 28 publications

(12 citation statements)

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“…K+, which is highly permeable, has an internal concentration of 78-9 mm (Huddart, 1966a). The K+ activity as measured with K+-sensitive micro-electrodes, 109-8 mm, appears to be greater than the concentration; this result is in agreement with the hypothesis that, as in barnacle muscle (McLaughlin & Hinke, 1966;Gayton, 1970), a portion of the internal fibre water may be bound with a consequent apparent increase in internal K+ activity. However, EK, -28-5 mV is still far less than the observed resting potential.…”

Section: Discussionsupporting

confidence: 82%

“…If, however, the activity coefficients of K+ inside and outside the muscle fibre are not the same, then using the ratio of the K+ concentrations to obtain ER does not give an accurate result. McLaughlin & Hinke (1966) and Gayton (1970) have described a system where signicant quantities of water are bound on the interior of the cell. If this is also the case in moth muscle, then the measurements of the total internal K+ [K+]o (mM) Fig.…”

Section: External and Internal K+ Activitiesmentioning

confidence: 99%

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The resting potential of moth muscle fibre

Rheuben¹

1972

The Journal of Physiology

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SUMMARY1. The membrane of the moth muscle fibre was tested for resting permeability to various ions: it is not permeable to Mg2+ or Ca2+; it is slightly permeable to Na+ and NH4+; it is appreciably permeable to Cl-, but C1-is passively distributed; it is apparently permeable to H+ but effects of HCO3-are not ruled out; and it is primarily permeable to K+.2. Measurement of the internal K+ activity showed that E. is less negative than the resting potential.3. In the presence of DNP, or under anoxia, the membrane potential approaches, EKE; there is a small concomitant decrease in effective membrane resistance.4. An increase in external Ca2+ concentration is accompanied by increased effective membrane resistance and an increase in amplitude of the negative resting potential.5. Cooling the membrane (below room temperature) decreased the amplitude of the resting potential by 4-16 mV per 10°C, and was accompanied by a large increase in effective membrane resistance.6. The experimental results most readily fit the hypothesis that the resting potential of the moth muscle fibre, although the membrane is highly permeable to K+, Cl-and apparently to H+, is primarily maintained by an electrogenic transport process which generates an ionic current across the membrane. The possibility that the concentration gradient of H+ ions is metabolically maintained at a level sufficient to explain the resting potential was considered to be unlikely but could not be directly excluded.

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Section: Discussionsupporting

confidence: 82%

Section: External and Internal K+ Activitiesmentioning

confidence: 99%

The resting potential of moth muscle fibre

Rheuben¹

1972

The Journal of Physiology

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“…The invaginations or clefts and the TTS create a barrier for diffusion which lengthens the time for the molecules to reach the whole cell membrane area. The volume of the TTS here reported of 10~o of the cell volume is slightly higher than the 6 [11] and 8 [12] percent values reported for other species.…”

Section: Discussioncontrasting

confidence: 77%

Permeability of barnacle muscle fibers to water and nonelectrolytes

1976

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The permeability of isolated muscle fibers of the giant barnacle Megabalanus psittacus to water and nonelectrolytes was studied by determining their reflection and permeability coefficients. Reflection coefficients were obtained by comparing the osmotic fluxes produced by a test molecule to that produced by the impermeant sucrose molecule. Permeability coefficients were determined for measurements of tracer uptake. The results indicate that, in these fibers, nonelectrolyte permeability is closely related to lipid solubility and molecular size. A value of 2.16 X 10(-12) cm3/sec dyne for the hydraulic conductivity and a value of 10.45 X 10(-4) cm/sec for 3HHO permeability coefficient were obtained. The effect of membrane surface invaginations and clefts on the determination of permeability coefficients is discussed.

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“…The ionic concentrations of these fibres were corrected for extra-fibre water space by taking the extra-fibre space as 6 %, a value reported by Gayton & Hinke (1968) to largely represent the giant cleft and transverse tubular system (TTS) present in barnacle fibres (vide P1. 1).…”

Section: Specimens Of Balanus Nubilusmentioning

confidence: 99%

An investigation of sodium transport in barnacle muscle fibres by means of the microsyringe technique

Bittar¹,

Chen²,

Danielson³

et al. 1972

The Journal of Physiology

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SUMMARY1. The cation composition of single barnacle muscle fibres following damage by axial insertion of a microsyringe has been measured. The Na and Ca contents of these fibres were raised.2. Electronmicroscopic studies of fibres following insertion of a microsyringe indicated that the damage done resulted in tubular obstruction of the T-system.3. Fibres loaded with radiosodium by micro-injection showed that the Na* efflux declined exponentially with time, but that in most fibres the Injections of distilled water deep in the fibre failed to influence the course of the Na* efflux.4. K removal reduced the Na efflux by 47 %. However, a few fibres displayed very little K-dependence.5. When measured in fibres already soaked in a K-free medium for long periods the sodium efflux consisted of a brief rapid phase, followed by a slow phase of Na loss.6. In the presence of 30 mM-K, there was little or no rise in the Na efflux. Raising the external K to 50 or 100 mm caused a marked rise in the Na efflux. Raising the external K to 30 mm in the absence of external Ca2+ led to a rise in the Na efflux. A high K solution always caused shortening of these fibres.7. Internal application of 1 M or 1 mM-CaCl2 often caused a significant rise in the Na efflux.8. Internal application of 2x5 or 5 M saline caused a prompt and large t Permanent address: Institute of Physiology and Medical Biophysics, University of Uppsala, Uppsala, Sweden. E. EDWARD BITTAR AND OTHERS fall in the Na efflux. In the presence of high K saline-loaded fibres failed to contract.9. Internal application of 0-5 M-ATP stimulated the Na efflux. A larger effect was not observed in fibres pre-treated with 2 M-MgCl2. Internal application of 0 5 m-ArP was without effect.10. The results indicate that the barnacle fibre is a suitable preparation for the study of Na fluxes by means of the micro-injection technique. They also indicate that the mechanism regulating the Na efflux is not quite the same as that found in squid axon or frog muscle.

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The location of chloride in single striated muscle fibers of the giant barnacle

Cited by 28 publications

References 0 publications

The resting potential of moth muscle fibre

The resting potential of moth muscle fibre

Permeability of barnacle muscle fibers to water and nonelectrolytes

An investigation of sodium transport in barnacle muscle fibres by means of the microsyringe technique

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