The final Middle Palaeolithic of northern Italy is almost exclusively known based on pluristratified sites in caves or rock shelter, which attest a certain technological variability within the Mousterian through the adoption of different knapping methods focused on the production of flakes or blades. The almost total lack of specialized and/or short-term open-air sites framed at this stage contributes to create a fragmentary and incomplete picture with regard to the last Neanderthal occupation of the area. For this reason, the Monte Netto site, an isolated hill at the northern margin of the Po Plain and at the foot of the Prealps, represents a key deposit to investigate this phase. Along the loess-palaeosoil sequence, investigated from a geochronological and pedological point of view, frequentations by Mousterian Neanderthal groups are attested at two different times, of which the most consistent is: associated to sediments dated to 44,400 ± 5.4 ky BP. The findings confirm the sporadic frequentation of marginal open areas during the cold, arid and highly fluctuating climate of the MIS 3, providing a more complete picture of the human occupation along the Po Plain Loess Basin (PPLB). An interregional comparison points out the clear intention, by the last Neanderthal groups of the Po Plain, of producing elongated supports by applying different methods, within a varied and organized system of resources exploitation evidenced by the selection of raw materials coming from more than 60 km.