Earthquakes are known to enhance permeability at great distances, and this phenomenon may also disrupt groundwater systems by breaching the barrier between different reservoirs. Here we analyze the tidal response of water level in a deep (~4 km) well before and after the 2008 M7.9 Wenchuan earthquake to show that the earthquake not only changed the permeability but also altered the poroelastic properties of the groundwater system. Based on lithologic well logs and experimental data for rock properties, we interpret the change to reflect a coseismic breaching of aquitards bounding the aquifer, due perhaps to clearing of preexisting cracks and creation of new cracks, to depths of several kilometers. This may cause mixing of groundwater from previously isolated reservoirs and impact the safety of groundwater supplies and underground waste repositories. The method demonstrated here may hold promise for monitoring aquitard breaching by both natural and anthropogenic processes.