The long noncoding RNA MyHC IIA/X-AS contributes to skeletal muscle myogenesis and maintains the fast fiber phenotype

Dou, Mingle; Yao, Ying; Ma, Lu; Wang, Xiaoyu; Shi, Xin’e; Yang, Gongshe

doi:10.1074/jbc.ra119.010498

Cited by 25 publications

(18 citation statements)

References 23 publications

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“…A miRNA pull-down assay was performed as previously reported ( 51 ). C2C12 cells were transfected with biotinylated miR-532-3p: wildtype miR-532-3p (miR-532-3p-Bio) or mutated miR-532-3p (G–C mutation in the lnc-ORA–binding sites, miR-532-3p-MutBio) and biotinylated control (negative control-Bio) when 70 to 80% of confluency was reached and induced into myogenic differentiation at full confluence.…”

Section: Methodsmentioning

confidence: 99%

Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis

Cai¹,

Zhang²,

Wang

et al. 2021

Journal of Biological Chemistry

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show abstract

Section: Methodsmentioning

confidence: 99%

Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis

Cai¹,

Zhang²,

Wang

et al. 2021

Journal of Biological Chemistry

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“…For example, elderly individuals with sarcopenia primarily present with a loss of type II fibres, whereas chronic obesity is primarily associated with type I fibre atrophy. Recently, a number of studies have investigated the potential role of lncRNAs in the regulation of muscle fibre type 89–92 and have been associated with a switch in MHC expression from IIx to IIa in humans following a 5‐week exercise protocol 92 . Thus, it will be important to identify if similar lncRNA‐mediated changes in fibre type occur in the obese state.…”

Section: Lncrnas and Obesity‐associated Chronic Inflammatory Diseasementioning

confidence: 99%

“…86 In a similar study, the novel atrophy-related lncRNA-1 (Atrolnc-1) was upregulated in the skeletal muscle of murine models of muscle wasting. 87 Furthermore, Atrolnc-1 was demonstrated to bind A20 binding inhibitor of NF-κB-1 (ABIN-1) in the cytoplasm of number of studies have investigated the potential role of lncRNAs in the regulation of muscle fibre type [89][90][91][92] and have been associated…”

Section: Sarcopenia and Skeletal Muscle Atrophymentioning

confidence: 99%

Involvements of long noncoding RNAs in obesity‐associated inflammatory diseases

et al. 2020

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Summary Obesity is associated with chronic low‐grade inflammation that affects the phenotype of multiple tissues and therefore is implicated in the development and progression of several age‐related chronic inflammatory disorders. Importantly, a new family of noncoding RNAs, termed long noncoding RNAs (lncRNAs), have been identified as key regulators of inflammatory signalling pathways that can mediate both pretranscriptional and posttranscriptional gene regulation. Furthermore, several lncRNAs have been identified, which are differentially expressed in multiple tissue types in individuals who are obese or in preclinical models of obesity. In this review, we examine the evidence for the role of several of the most well‐studied lncRNAs in the regulation of inflammatory pathways associated with obesity. We highlight the evidence for their differential expression in the obese state and in age‐related conditions including insulin resistance, type 2 diabetes (T2D), sarcopenia, osteoarthritis and rheumatoid arthritis, where obesity plays a significant role. Determining the expression and functional role of lncRNAs in mediating obesity‐associated chronic inflammation will advance our understanding of the epigenetic regulatory pathways that underlie age‐related inflammatory diseases and may also ultimately identify new targets for therapeutic intervention.

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“…Fibers expressing the slower MyHC isoforms (MyHC I and MyHC IIa) have greater resistance to fatigue, are rich in mitochondria and mainly rely on oxidative phosphorylation to produce energy. Differently, fibers expressing faster MyHC (MyHC IIx, MyHC IIb) show reduced mitochondrial content and mostly take advantage of glycolytic metabolism [ 33 , 34 ]. In order to analyze whether proNGF treatment could modulate fiber type specification during myogenic differentiation, SCDMs were allowed to differentiate in the DM in the presence or absence of proNGF, and prospective protein changes were analyzed at 6, 24 and 48 h of differentiation.…”

Section: Resultsmentioning

confidence: 99%

ProNGF/p75NTR Axis Drives Fiber Type Specification by Inducing the Fast-Glycolytic Phenotype in Mouse Skeletal Muscle Cells

Pallottini

Colardo

Tonini

et al. 2020

Cells

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Despite its undisputable role in the homeostatic regulation of the nervous system, the nerve growth factor (NGF) also governs the relevant cellular processes in other tissues and organs. In this study, we aimed at assessing the expression and the putative involvement of NGF signaling in skeletal muscle physiology. To reach this objective, we employed satellite cell-derived myoblasts as an in vitro culture model. In vivo experiments were performed on Tibialis anterior from wild-type mice and an mdx mouse model of Duchenne muscular dystrophy. Targets of interest were mainly assessed by means of morphological, Western blot and qRT-PCR analysis. The results show that proNGF is involved in myogenic differentiation. Importantly, the proNGF/p75NTR pathway orchestrates a slow-to-fast fiber type transition by counteracting the expression of slow myosin heavy chain and that of oxidative markers. Concurrently, proNGF/p75NTR activation facilitates the induction of fast myosin heavy chain and of fast/glycolytic markers. Furthermore, we also provided evidence that the oxidative metabolism is impaired in mdx mice, and that these alterations are paralleled by a prominent buildup of proNGF and p75NTR. These findings underline that the proNGF/p75NTR pathway may play a crucial role in fiber type determination and suggest its prospective modulation as an innovative therapeutic approach to counteract muscle disorders.

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The long noncoding RNA MyHC IIA/X-AS contributes to skeletal muscle myogenesis and maintains the fast fiber phenotype

Abstract: Mammalian skeletal muscles comprise different types of muscle fibers, and this muscle fiber heterogeneity is generally characterized marked by the expression of myosin heavy chain (MyHC) isoforms. A INTRODUCTIONhttps://www.jbc.org/cgi/

Cited by 25 publications

References 23 publications

Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis

Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis

Involvements of long noncoding RNAs in obesity‐associated inflammatory diseases

ProNGF/p75NTR Axis Drives Fiber Type Specification by Inducing the Fast-Glycolytic Phenotype in Mouse Skeletal Muscle Cells

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