The Long-term Fate of Fresh and Frozen Orthotopic Bone Allografts in Genetically Defined Rats

Gd, Bos; Vm, Goldberg; Nh, Gordon; Bm, Dollinger; Jm, Zika; Ae, Powell; Kg, Heiple

doi:10.1097/00003086-198507000-00031

Cited by 21 publications

(8 citation statements)

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“…Rinsing the graft also removes most of the marrow, allowing a reduction in the immunological load to the patient. Allograft produces an immunogenic response to the graft 49 and most antigenic cells are found in the marrow. 50,51 Furthermore, the risk of disease transmission is lessened with removal of marrow and cells.…”

Section: Rinsing Of Graftmentioning

confidence: 99%

Impaction allografting in revision total hip replacement

Board

Rooney²,

Kearney³

et al. 2006

The Journal of Bone and Joint Surgery. British volume

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Section: Rinsing Of Graftmentioning

confidence: 99%

Impaction allografting in revision total hip replacement

Board

Rooney²,

Kearney³

et al. 2006

The Journal of Bone and Joint Surgery. British volume

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“…Revascularization and incorporation of the graft thus delayed, non-union and fractures are frequent (1, [3][4][5]8,15,19,21,24). Despite the negative aspects of allograft transplantation, these grafts often are desirable clinically: they are readily obtainable and can be size matched for best fit, and their use eliminates the necessity for a second harvest operation, which increases patient morbidity.…”

mentioning

confidence: 99%

The effects of cisplatin on the incorporation of fresh syngeneic and frozen allogeneic cortical bone grafts

Zart

Miya

Wolff

et al. 1993

Journal Orthopaedic Research

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Allograft transplantation with concomitant chemotherapy has proven successful in the treatment of malignant bone tumors. However, these chemotherapeutic agents may delay tissue healing, resulting in clinical complications. To clarify the effects of cisplatin on the healing of bone grafts, we studied the incorporation of stably fixed massive diaphyseal femoral syngeneic and allogeneic grafts in rats treated with cisplatin. These data were compared with those of historical controls from animals that did not receive cisplatin. Rats that were to receive a fresh syngeneic graft or frozen allogeneic graft were given cisplatin every 4 weeks starting 9 weeks preoperatively and continuing until the time of death. The total bone area of the graft in animals that received cisplatin was smaller than that of the graft in untreated control rats that did not receive cisplatin. The area of the frozen allograft did not increase between 2 and 4 months. Revascularization was incomplete in cisplatin-treated groups at 2 months, but by 4 months, vessel ingrowth in fresh syngeneic grafts approached control values. Frozen allografts remained poorly revascularized at 4 months. Host-graft union was poor at 2 months in cisplatin-treated rats compared with controls. In cisplatin-treated rats, the host-graft union of the frozen allograft remained inferior at 4 months while that of the syngeneic graft improved. Allogeneic cortical bone grafts are incorporated more slowly and incompletely than syngeneic grafts, and this handicap is exacerbated by the administration of cisplatin.

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“…The most efficient tissue source would be the autologous bone, but the donor-site morbidity, the inadequate supply, and the problems about size and shape make this source not always feasible. Allografts from cadaveric donors are increasingly used, but the risk of disease transmission and/or immune reaction limit their use [ 33 , 34 ]. Indeed, allogeneic peptides may be presented by APC, such as DC, on MHC-I and MHC-II molecules and can activate cytotoxic CD8 + T cells and CD4 + helper T cells.…”

Section: Discussionmentioning

confidence: 99%

The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System

Trabanelli

Manna

Romano

et al. 2015

Journal of Immunology Research

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In vitro differentiation of mesenchymal stromal cells (MSC) into osteocytes (human differentiated osteogenic cells, hDOC) before implantation has been proposed to optimize bone regeneration. However, a deep characterization of the immunological properties of DOC, including their effect on dendritic cell (DC) function, is not available. DOC can be used either as cellular suspension (detached, Det-DOC) or as adherent cells implanted on scaffolds (adherent, Adh-DOC). By mimicking in vitro these two different routes of administration, we show that both Det-DOC and Adh-DOC can modulate DC functions. Specifically, the weak downregulation of CD80 and CD86 caused by Det-DOC on DC surface results in a weak modulation of DC functions, which indeed retain a high capacity to induce T-cell proliferation and to generate CD4+CD25+Foxp3+ T cells. Moreover, Det-DOC enhance the DC capacity to differentiate CD4+CD161+CD196+ Th17-cells by upregulating IL-6 secretion. Conversely, Adh-DOC strongly suppress DC functions by a profound downregulation of CD80 and CD86 on DC as well as by the inhibition of TGF-β production. In conclusion, we demonstrate that different types of DOC cell preparation may have a different impact on the modulation of the host immune system. This finding may have relevant implications for the design of cell-based tissue-engineering strategies.

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The Long-term Fate of Fresh and Frozen Orthotopic Bone Allografts in Genetically Defined Rats

Cited by 21 publications

References 0 publications

Impaction allografting in revision total hip replacement

Impaction allografting in revision total hip replacement

The effects of cisplatin on the incorporation of fresh syngeneic and frozen allogeneic cortical bone grafts

The Human Mesenchymal Stromal Cell-Derived Osteocyte Capacity to Modulate Dendritic Cell Functions Is Strictly Dependent on the Culture System

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