The loss of female sex pheromone after mating in the corn earworm moth Helicoverpa zea: identification of a male pheromonostatic peptide.

Kingan, Timothy G.; Bodnar, Wanda M.; Raina, Ashok K.; Shabanowitz, Jeffrey; Hunt, Donald F.

doi:10.1073/pnas.92.11.5082

Cited by 104 publications

(76 citation statements)

References 14 publications

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“…In a process called pheromonostasis, females in a number of moth species cease production of a sex pheromone after mating with males. In the corn earworm moth, Helicoverpa zea, pheromonostatic action has been attributed to a 57-aa pheromone-suppression peptide (13) that is present in the male seminal fluid. Indeed, the fruit fly, Drosophila melanogaster, has emerged as an important model for understanding the nature and specific function of a large number of male accessory-gland protein genes (Acps) that are required for this pheromonostatic control (12).…”

Section: Discussionmentioning

confidence: 99%

The species, sex, and stage specificity of a Caenorhabditis sex pheromone

Chasnov¹,

Chan

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

123

184

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Four species in the ELEGANS group of the subgenus Caenorhabditis are distinguished by two very different mating systems: androdioecy in C. elegans and Caenorhabditis briggsae with males and self-fertilizing hermaphrodites and dioecy in Caenorhabditis remanei and Caenorhabditis sp. strain CB5161 with males and females. Using chemotaxis assays, we demonstrate that females secrete a potent sex pheromone that attracts males from a distance, whereas hermaphrodites do not. The female sex pheromone is not species-specific, with males of all four species attracted to both the C. remanei and Caenorhabditis sp. female sex pheromones. The pheromone is, however, sex-specific, with only females secreting the pheromone and attracting only males. Furthermore, the sex pheromone is stage-specific, with female secretion and male detection of the pheromone beginning near adulthood. Females lose their attractiveness immediately after mating but regain it several hours after mating ceases. Finally, the female somatic gonad is required for sex-pheromone production, and the male-specific cephalic neurons (CEM) are required for male response. chemotaxis ͉ cephalic neuron ͉ sex attractant ͉ nematode mating D irect observations show that the mating efficiency of the androdioecious species Caenorhabditis elegans is poor compared with the related dioecious species Caenorhabditis remanei and that both C. remanei and C. elegans males find C. remanei females more attractive than C. elegans hermaphrodites (1). Perhaps during evolution C. elegans hermaphrodites lost the ability to secrete a sex pheromone that is still secreted by C. remanei females and this pheromone still attracts C. elegans males. The focus of the present study is to definitively determine the presence of a sex pheromone in dioecious Caenorhabditis that is absent in related hermaphroditic species.Although C. elegans is a well-studied organism, it is yet debatable whether there is a true sex pheromone in this species, although apparently males can sense a hermaphrodite-derived chemical (2, 3). If hermaphrodites do indeed secrete a true sex pheromone, it is surprising that males do not rapidly and consistently chemotax to hermaphrodites, because C. elegans is capable of chemotaxing to both water-soluble and volatile chemicals (4). In fact, detection of sex pheromones secreted by other nematode species has been fairly straightforward (5), with the secreting female commonly establishing a gradient of attractant readily followed by conspecific males.Females, in contrast to self-fertilizing hermaphrodites, must mate with males to reproduce, and natural selection should favor those females that do so rapidly and consistently after reaching adulthood. Here, we demonstrate that females from the dioecious species C. remanei and Caenorhabditis sp. strain CB5161 attract conspecific males from a distance, whereas hermaphrodites from the androdioecious species C. elegans and Caenorhabditis briggsae do not. We then obtain, by soaking females, a supernatant solution that can attract males fr...

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Section: Discussionmentioning

confidence: 99%

The species, sex, and stage specificity of a Caenorhabditis sex pheromone

Chasnov¹,

Chan

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

123

184

View full text Add to dashboard Cite

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“…This response is triggered by chemicals in the seminal fluid, and it has been shown that injection of combined extracts of accessory glands and ejaculatory ducts blocks the synthesis of female sex pheromone (Kingan et al, 1993). The most potent component in these extracts was a 6.6 kDa peptide (Kingan et al, 1995). Pheromone synthesis in females is regulated by pheromone biosynthesis-activating neuropeptide (PBAN), released from the brain during the scotophase (Raina, 1993).…”

Section: Mating Plugs -From Mechanical To Physiological Manipulationmentioning

confidence: 99%

She’s my girl - male accessory gland products and their function in the reproductive biology of social bees

Colonello-Frattini

Hartfelder

2005

Apidologie

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-Male accessory gland products have become a major issue in insect reproduction. They are a means of transport for sperm and can form a mating plug, and also have specific compounds that can modify the behavior and physiology of mated females. We briefly review the structure and function of accessory gland products in insects, especially in the fruitfly and some orthopterans and lepidopterans, and draw parallels to what is currently known in social bees. The structure of the mating sign differs considerably in this group. In bumble bees it consists of a viscous mass of a dipeptide and fatty acids, with linoleic acid affecting female behavior. The honey bees show considerable species-specific variation in their mating signs. In the cavity-dwelling species it is the mucus gland which provides the mass of the mating sign, and this glands undergoes a hormonally controlled sexual maturation in its program of protein synthesis. The stingless bees lack mucus glands altogether, and their mating sign consists of the ruptured genital capsule of the male. We discuss the structure of the mating sign and of its components in relation to the mating and reproductive biology of these groups of highly eusocial bees.honeybee / bumblebee / Meliponini / reproductive tract / mating sign / Apis / Bombus

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“…gypsy moths, Giebultowicz et al, 1991; silk moths, Karube and Kobayashi, 1999). Both the spermatheca and the bursa copulatrix are innervated, indicating that sperm may play a role in switching off female receptivity and stimulating oviposition (Sugawara, 1979;Kingan et al, 1995). In the butterfly Pieris rapae, for example, the presence of sperm in the spermatheca appears to cause neural triggering of female unreceptivity (Obara et al, 1975).…”

Section: Spermmentioning

confidence: 99%

“…In Helicoverpa zea, pheromone production is switched off by a peptide originating from the male accessory glands (Kingan et al, 1995), whilst in the closely related species Heliothis virescens, a testicular factor, most likely the ecdysteroid 20E, is responsible (Ramaswamy and Cohen, 1992;Ramaswamy et al, 1996). In Choristoneura rosaceana, despite mating resulting in increased levels of JH in females and suppressed pheromone production, this JH does not originate from the male, as they lack the ability to synthesize and store JH in their accessory glands (Cusson et al, 1999).…”

Section: Seminal Fluidsmentioning

confidence: 99%

Female receptivity in butterflies and moths

Wedell

2005

Journal of Experimental Biology

136

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SUMMARY Female receptivity in butterflies and moths is influenced by a multitude of factors that vary between virgin and mated females, and is often affected by the quality and persistence of courting males. Mated females of polyandrous species frequently display a period of non-receptivity following mating, often resulting from factors transferred by the male at mating. Some of these compounds have a transient effect (e.g. anti-aphrodisiacs and mating plugs),whereas others induce long-term suppression of receptivity (i.e. sperm and seminal factors). Sperm appear to generally induce long-term suppression of female receptivity in both butterflies and moths. In some species, production of non-fertile sperm may function to fill the female's sperm storage organ and switch off receptivity, although whether this is a general phenomenon across the Lepidoptera has not yet been examined. Examination of seminal fluids suppressing female receptivity in moths suggests that more than one factor is implicated, but frequently the transfer or stimulation of Juvenile Hormone production is involved. Surprisingly, potential seminal factors influencing female receptivity in butterflies remain largely unexplored. In this review, I summarize the various factors that are known to affect female receptivity in the Lepidoptera to date, and briefly compare the function and similarity of the Pheromone Suppressing Peptide (HezPSP) in moths to that of the Sex Peptide in Drosophila melanogaster (DrmSP). The exciting possibility that seminal peptides in the Lepidoptera and Diptera (e.g. Drosophila melanogaster) may have shared functionality is discussed.

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The loss of female sex pheromone after mating in the corn earworm moth Helicoverpa zea: identification of a male pheromonostatic peptide.

Cited by 104 publications

References 14 publications

The species, sex, and stage specificity of a Caenorhabditis sex pheromone

The species, sex, and stage specificity of a Caenorhabditis sex pheromone

She’s my girl - male accessory gland products and their function in the reproductive biology of social bees

Female receptivity in butterflies and moths

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