The &lt;i&gt;FT-like&lt;/i&gt; gene &lt;i&gt;PehFT&lt;/i&gt; in petunia responds to photoperiod and light quality but is not the main gene promoting light quality-associated flowering

Tsukamoto, Atsuko; Hirai, Tadayoshi; Chin, Dong Poh; Mii, Masahiro; Mizoguchi, Tsuyoshi; Mizuta, Daiki; Yoshida, Hideo; Olsen, Jorunn E.; Ezura, Hiroshi; Fukuda, Naoya

doi:10.5511/plantbiotechnology.16.0620a

Cited by 13 publications

(10 citation statements)

References 41 publications

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“…; Tsukamoto et al . ). In the case where vegetative plant products are desired, such as herbs, specific light treatments can be implemented to suppress flowering or increase biomass (Stagnari et al .…”

Section: Market‐timed Productionmentioning

confidence: 97%

“…The precise timing of production is attractive to growers as they can reach counter-seasonal markets where produce may command a higher retail price. Manipulation of the light environment may provide a means to alter developmental transition to flowering, as light quality has been shown to affect the process in fruit crops (Rantanen et al 2014;Yoshida et al 2016) and flowers (Park et al 2015;Tsukamoto et al 2016). In the case where vegetative plant products are desired, such as herbs, specific light treatments can be implemented to suppress flowering or increase biomass (Stagnari et al 2018).…”

Section: Market-timed Productionmentioning

confidence: 99%

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Breeding new varieties for controlled environments

Folta

2018

Plant Biol J

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Agricultural production in controlled environments is increasingly feasible, and may play an important role in providing nutrition and choice to growing urban centres. New technologies in lighting, ventilation, robotics and irrigation are just a few of the innovations that enable production of high-value specialty crops outside of a traditional field setting. However, despite all of the advances in the hardware within the plant factory operation, innovation of the most complex machine has been neglected - the plant itself. Indoor agricultural operations typically rely on legacy varieties, plants selected and bred for field conditions. In the field, phenotypic stability is paramount, as production must be consistent in an unpredictable and changing environment. However, the controlled environment affords focus on different breeding priorities as environmental flux, pests, pathogens and post-harvest quality are less formidable barriers to production. On the contrary, breeding for controlled environments shifts the focus to a completely different set of plant traits, such as rapid growth, performance in low light environments and active manipulation of plant stature. Instead of breeding for phenotypic stability, plants may be bred to maximise genetic plasticity, allowing specific traits to be presented as a function of the quality of the ambient light spectrum. In this scenario plant varieties may be grown with optimal size, supporting a focus on consumer traits like flavour or accumulation of health-related compounds. Gene editing may be a central technology in the production of designer plants for controlled environments. This review considers the opportunity for breeding for controlled environments, with a focus on a revision of priorities for controlled-environment breeders.

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Section: Market‐timed Productionmentioning

confidence: 97%

Section: Market-timed Productionmentioning

confidence: 99%

Breeding new varieties for controlled environments

Folta

2018

Plant Biol J

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“…In A. thaliana, SOC1 has a role in transmitting a signal from the FT protein to induce floral bud formation. In another previous study of ours, PehFT, a FT-like gene from petunia, was identified [16] but involvement of PehFT in the floral induction control by light quality was not revealed. Petunia is a typical long-day plant showing strong responses to environmental changes, including changes in light quality and irradiance, with respect to floral induction as well as morphogenesis [15].…”

Section: Introductionmentioning

confidence: 71%

“…In our previous studies [15,16], flowering of petunia (Petunia × hybrida) was shown to be delayed in plants grown under R LEDs and accelerated in plants grown under B LEDs. In addition, the presence of PehFT genes was reported, but their function in promotion of flowering is unclear since the expression of PehFT was not increased under the B light conditions [16]. In this study, we aimed to identify genes associated with the regulation of flowering time by light quality in petunia.…”

Section: Discussionmentioning

confidence: 95%

“…In addition, Haliapas et al, [14] reported that high irradiance and FR end-of-day (EOD) treatment induce floral bud formation in petunia. Furthermore, our previous reports [15,16] have shown that the flowering of petunia (Petunia × hybrida) is delayed in plants grown under R light from LEDs and accelerated in plants grown under blue LEDs. However, it is still unclear which floral induction-and signal transduction genes are involved in the regulation of flowering in petunia under different light qualities.…”

Section: Introductionmentioning

confidence: 95%

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The Expression of ELF4-Like Genes Is Influenced by Light Quality in Petunia

Fukuda

Suenaga

Miura³

et al. 2020

Agronomy

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The signals from photoreceptors modify plant morphogenesis and regulate the timing of flowering. In the long-day plant petunia, flowering is accelerated under blue (B) and white (W) light compared to red (R) light. In Arabidopsis thaliana L., ELF genes are involved in circadian clock-associated regulation of flowering under different light conditions. In this study, we aimed to assess the involvement of ELF genes in control of flowering by light quality in petunia. Two ELF4-like genes, PhELF4-1 and PhELF4-2 with 76% and 70% similarity to orthologues in pepper but low overall similarity to ELF genes in A. thaliana L., were characterized in petunia and their expression patterns studied under different light qualities. Both genes showed a rhythmic expression pattern and higher expression under B light from light emitting diodes (LED) and W light from fluorescent lamps than under R LED light from LED. For both genes, the expression peaked towards the end of the day, 12 h after start of a 14 h photoperiod. Compared with PhELF4-2, PhELF4-1 expression showed higher amplitude with significantly higher peak expression. As investigated for PhELF4-1, such an expression rhythm was kept for two days after transfer of the plants to continuous lighting using B LED, indicating a circadian rhythm. PhELF4-1 also responded with a phase shift after transfer to short days of an 8 h photoperiod. These results indicate that PhELF4-like genes in petunia are under photoperiodic control involving a circadian clock and play a role in signal transduction from one or more B light photoreceptors.

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CRISPR/Cas9-mediated mutagenesis of FT/TFL1 in petunia improves plant architecture and early flowering

Abdulla,

Mostafa,

Kavas

2024

Plant Mol Biol

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Petunias are renowned ornamental species widely cultivated as pot plants for their aesthetic appeal both indoors and outdoors. The preference for pot plants depends on their compact growth habit and abundant flowering. While genome editing has gained significant popularity in many crop plants in addressing growth and development and abiotic and biotic stress factors, relatively less emphasis has been placed on its application in ornamental plant species. Genome editing in ornamental plants opens up possibilities for enhancing their aesthetic qualities, offering innovative opportunities for manipulating plant architecture and visual appeal through precise genetic modifications. In this study, we aimed to optimize the procedure for an efficient genome editing system in petunia plants using the highly efficient multiplexed CRISPR/Cas9 system. Specifically, we targeted a total of six genes in Petunia which are associated with plant architecture traits, two paralogous of FLOWERING LOCUS T (PhFT) and four TERMINAL FLOWER-LIKE1 (PhTFL1) paralogous genes separately in two constructs. We successfully induced homogeneous and heterogeneous indels in the targeted genes through precise genome editing, resulting in significant phenotypic alterations in petunia. Notably, the plants harboring edited PhTFL1 and PhFT exhibited a conspicuously early flowering time in comparison to the wild-type counterparts. Furthermore, mutants with alterations in the PhTFL1 demonstrated shorter internodes than wild-type, likely by downregulating the gibberellic acid pathway genes PhGAI, creating a more compact and aesthetically appealing phenotype. This study represents the first successful endeavor to produce compact petunia plants with increased flower abundance through genome editing. Our approach holds immense promise to improve economically important potting plants like petunia and serve as a potential foundation for further improvements in similar ornamental plant species.

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The <i>FT-like</i> gene <i>PehFT</i> in petunia responds to photoperiod and light quality but is not the main gene promoting light quality-associated flowering

Cited by 13 publications

References 41 publications

Breeding new varieties for controlled environments

Breeding new varieties for controlled environments

The Expression of ELF4-Like Genes Is Influenced by Light Quality in Petunia

CRISPR/Cas9-mediated mutagenesis of FT/TFL1 in petunia improves plant architecture and early flowering

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