The Ly45.1 alloantigen: preferential expression on CD4 - CD8 - /CD4 + CD8 + thymocytes

Tomonari, Kyuhei; Fairchild, Susan P.; Rosenwasser, Oliver A.; Tada, Nobuhiko

doi:10.1007/s002510050434

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“…The following antibodies were used in this study: fluorescein isothiocyanate (FITC)-labeled anti-V ␤ 4 (KT4-10 [39]), FITC-labeled anti-V ␤ 5 (MR9-4; Pharmingen, San Diego, Calif.), FITC-labeled anti-V ␤ 11 (RR3-15; Pharmingen), FITC-labeled anti-V ␤ 12 (MR11-1; Pharmingen), Cychrome-coupled anti-CD4 (RM4-5; Pharmingen), and phycoerythrin-coupled anti-CD69 (H1.2F3; Pharmingen). Anti-V ␤ 10 a/c (KT10a [38]), kindly provided by K. Tomonari (Fukui Medical School, Fukui, Japan), was used as a hybridoma supernatant and was detected by an FITC-coupled goat anti-rat immunoglobulin G (IgG). A polyclonal rabbit anti-open reading frame-peptide serum (serum C [7]) raised against a 23-aa synthetic peptide of the MMTV(GR) Sag was used for immunoprecipitation of the viral Sags expressed by RVs.…”

Section: Mousementioning

confidence: 99%

Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor V_βDomains

Baribaud

Wirth

Maillard

et al. 2001

J Virol

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Mouse mammary tumor virus (MMTV) is a retrovirus encoding a superantigen that is recognized in association with major histocompatibility complex class II by the variable region of the beta chain (V ␤ ) of the T-cell receptor. The C-terminal 30 to 40 amino acids of the superantigen of different MMTVs display high sequence variability that correlates with the recognition of particular T-cell receptor V ␤ chains. Interestingly, MMTV(SIM) and mtv-8 superantigens are highly homologous but have nonoverlapping T-cell receptor V ␤ specificities. To determine the importance of these few differences for specific V ␤ interaction, we studied superantigen responses in mice to chimeric and mutant MMTV(SIM) and mtv-8 superantigens expressed by recombinant vaccinia viruses. We show that only a few changes (two to six residues) within the C terminus are necessary to modify superantigen recognition by specific V ␤ s. Thus, the introduction of the MMTV(SIM) residues 314-315 into the mtv-8 superantigen greatly decreased its V ␤ 12 reactivity without gain of MMTV(SIM)-specific function. The introduction of MMTV(SIM)-specific residues 289 to 295, however, induced a recognition pattern that was a mixture of MMTV(SIM)-and mtv-8-specific V ␤ reactivities: both weak MMTV(SIM)-specific V ␤ 4 and full mtv-8-specific V ␤ 11 recognition were observed while V ␤ 12 interaction was lost. The combination of the two MMTV(SIM)-specific regions in the mtv-8 superantigen established normal MMTV(SIM)-specific V ␤ 4 reactivity and completely abolished mtv-8-specific V ␤ 5, -11, and -12 interactions. These new functional superantigens with mixed V ␤ recognition patterns allowed us to precisely delineate sites relevant for molecular interactions between the SIM or mtv-8 superantigen and the T-cell receptor V ␤ domain within the 30 C-terminal residues of the viral superantigen.

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Section: Mousementioning

confidence: 99%

Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor V_βDomains

Baribaud

Wirth

Maillard

et al. 2001

J Virol

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The Ly45.1 alloantigen: preferential expression on CD4 - CD8 - /CD4 + CD8 + thymocytes

Cited by 1 publication

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Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor V_βDomains

Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor V_βDomains

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The Ly45.1 alloantigen: preferential expression on CD4 - CD8 - /CD4 + CD8 + thymocytes

Cited by 1 publication

References 0 publications

Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor VβDomains

Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor VβDomains

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Product

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Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor V_βDomains

Identification of Key Amino Acids of the Mouse Mammary Tumor Virus Superantigen Involved in the Specific Interaction with T-Cell Receptor V_βDomains