The Lysis of Pathogenic Escherichia coli by Bacteriophages Releases Less Endotoxin Than by β-Lactams

Dufour, Nicolas; Delattre, Raphaëlle; Ricard, Jean‐Damien; Debarbieux, Laurent

doi:10.1093/cid/cix184

Cited by 77 publications

(55 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, in this particular case, we overcame these hurdles and did not observe any discernible adverse clinical events. For example, concerns have arisen about the possibility that an accelerated lysis of Gram-negative bacterial pathogens could release clinically significant levels of endotoxin (27,28). A parallel concern can be made for administration of some antibiotics or any other alternative treatment that could lead to rapid, widespread cell lysis.…”

Section: Discussionmentioning

confidence: 99%

Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection

Schooley

Biswas

Gill

et al. 2017

Antimicrob Agents Chemother

927

811

View full text Add to dashboard Cite

Widespread antibiotic use in clinical medicine and the livestock industry has contributed to the global spread of multidrug-resistant (MDR) bacterial pathogens, including Acinetobacter baumannii. We report on a method used to produce a personalized bacteriophage-based therapeutic treatment for a 68-year-old diabetic patient with necrotizing pancreatitis complicated by an MDR A. baumannii infection. Despite multiple antibiotic courses and efforts at percutaneous drainage of a pancreatic pseudocyst, the patient deteriorated over a 4-month period. In the absence of effective antibiotics, two laboratories identified nine different bacteriophages with lytic activity for an A. baumannii isolate from the patient. Administration of these bacteriophages intravenously and percutaneously into the abscess cavities was associated with reversal of the patient's downward clinical trajectory, clearance of the A. baumannii infection, and a return to health. The outcome of this case suggests that the methods described here for the production of bacteriophage therapeutics could be applied to similar cases and that more concerted efforts to investigate the use of therapeutic bacteriophages for MDR bacterial infections are warranted.

show abstract

Section: Discussionmentioning

confidence: 99%

Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection

Schooley

Biswas

Gill

et al. 2017

Antimicrob Agents Chemother

927

811

View full text Add to dashboard Cite

show abstract

“…Theoretically, the use of lytic phages can induce a rapid bacterial lysis, which can lead to a release of endotoxin, possibly causing a Jarisch‐Herxheimer reaction or an immunologic reaction to the release of endotoxin during antibiotic treatment . However, lytic phages induce less endotoxin release than B‐lactam antibiotics . Close follow‐up of the effect of phage therapeutic products in the human host is essential.…”

Section: Phage Therapymentioning

confidence: 99%

Review article: bacteriophages in gastroenterology—from biology to clinical applications

Sabino

Hirten

Colombel

2019

Aliment Pharmacol Ther

View full text Add to dashboard Cite

Summary Background The gut microbiota plays an important role in the pathogenesis of several gastrointestinal diseases. Its composition and function are shaped by host‐microbiota and intra‐microbiota interactions. Bacteriophages (phages) are viruses that target bacteria and have the potential to modulate bacterial communities. Aims To summarise phage biology and the clinical applications of phages in gastroenterology Methods PubMed was searched to identify relevant studies. Results Phages induce bacterial cell lysis, integration of viral DNA into the bacteria and/or coexistence in a stable equilibrium. Bacteria and phages have co‐evolved and their dynamic interactions are yet to be fully understood. The increasing need to modulate microbial communities (e.g., gut microbiota, multidrug‐resistant bacteria) has been a strong stimulus for research in phages as an antibacterial therapy. In gastroenterology, phage therapy has been mainly studied in infectious diseases such as cholera. However, it is currently being explored in several other circumstances such as treating Clostridioides difficile colitis, targeting adherent‐invasive Escherichia coli in Crohn's disease or eradicating Fusobacterium nucleatum in colorectal cancer. Overall, phage therapy has a favourable and acceptable safety profile. Presently, trials with phage therapy are ongoing in Crohn's disease. Conclusions Phage therapy is a promising therapeutic tool against pathogenic bacteria in the fields of infectious diseases and gastroenterology. Randomised, placebo‐controlled trials with phage therapy for gastroenterological diseases are ongoing.

show abstract

“…Several studies have observed waterborne endotoxin release during inactivation of Gram-negative bacteria (Dufour et al, 2017;Zhang et al, 2016;Sreeja and Shetty, 2016). The results of these works verify that embedded endotoxins can be released into the environment after the death of bacteria or cells.…”

Section: Introductionmentioning

confidence: 99%

Inactivation of airborne bacteria using different UV sources: Performance modeling, energy utilization, and endotoxin degradation

Wang

Zhang

2019

Science of The Total Environment

View full text Add to dashboard Cite

Airborne bacteria-containing bioaerosols have attracted increased research attention on account of their adverse effects on human health. Ultraviolet germicidal irradiation (UVGI) is an effective method to inactivate airborne microorganisms. The present study models and compares the inactivation performance of three UV sources in the UVGI for aerosolized Escherichia coli. Inactivation efficiency of 0.5, 2.2 and 3.1 logarithmic order was obtained at an exposure UV dose of 370 J/m 3 under UVA (365 nm), UVC (254 nm) and UVD (185 nm) sources, respectively. A Beer-Lambert law-based model was developed and validated to compare the inactivation performances of the UV sources, and modeling enabled prediction of inactivation efficiency and analysis of the sensitivity of several parameters. Low influent E. coli concentrations and high UV doses resulted in high energy consumption (EC). The change in airborne endotoxin concentration during UV inactivation was analyzed, and UVC and UVA irradiation showed no marked effect on endotoxin degradation. By contrast, both free and bound endotoxins could be removed by UVD treatment, which is attributed to the ozone generated by the UVD source. The results of this study can provide a better understanding of the air disinfection and airborne endotoxin removal processes.

show abstract

The Lysis of Pathogenic Escherichia coli by Bacteriophages Releases Less Endotoxin Than by β-Lactams

Abstract: SummaryBacteriophages kill bacteria very rapidly. Bacteriophage-induced endotoxin release could be an issue during phage therapy. We show that bacteriophages stop cell growth more rapidly than do β-lactams, resulting in a lower endotoxin release.

Cited by 77 publications

References 47 publications

Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection

Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection

Review article: bacteriophages in gastroenterology—from biology to clinical applications

Inactivation of airborne bacteria using different UV sources: Performance modeling, energy utilization, and endotoxin degradation

Contact Info

Product

Resources

About