The magic and mystery of MicroRNA-27 in atherosclerosis

Chen, Wujun; Yin, Kai; Zhao, Guo-Jun; Fu, Yue; Tang, Chao-Ke

doi:10.1016/j.atherosclerosis.2012.01.020

Cited by 110 publications

(69 citation statements)

References 66 publications

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“…Cells or murine tissues were collected for protein extraction and detection of ABCA1, ABCG1, PPARγ and LXRα, as described previously [21]. The concentration of proteins was measured using a BCA protein assay kit (CWBIO, Peking, China).…”

Section: Methodsmentioning

confidence: 99%

Leonurine Prevents Atherosclerosis Via Promoting the Expression of ABCA1 and ABCG1 in a Pparγ/Lxrα Signaling Pathway-Dependent Manner

Jiang

Ren

Chen

et al. 2017

Cell Physiol Biochem

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Background/Aims: Previous studies have demonstrated that leonurine, a unique alkaloid compound of Herba leonuri, can exert anti-oxidative and anti-inflammatory effects on the development of atherosclerosis (AS). This study was designed to investigate the effects of leonurine on cholesterol efflux from THP-1 macrophage-derived foam cells and development of atherosclerotic lesions in apoE^-/- mice, and further determine the potential mechanisms. Methods: Human THP-1 cells were fully differentiated into foam cells by the pre-treatment with phorbol-12-myristate-13-acetate (PMA) and oxidized density lipoproteins (ox-LDL). After cells were incubated with various concentrations of leonurine, Oil Red O staining and high-performance liquid chromatography (HPLC) assays were utilized to detect cellular lipid accumulation and cholesterol content, respectively. Cellular cholesterol efflux was determined by liquid scintillation counting. The mRNA and protein levels of ATP-binding cassette transporter A1/G1 (ABCA1/G1), peroxisome proliferator-activated receptor γ (PPARγ) and liver X receptor α (LXRα) in foam cells were assessed using real-time quantitative PCR (RT-qPCR) and western blot analyses, respectively. Plasma triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) levels in apoE^-/- mice were evaluated using enzymatic methods. The atherosclerotic lesion sizes and collagen contents in aortic roots were determined by Oil Red O and Masson’s trichrome staining, respectively. Results: Oil Red O staining and liquid scintillation counting assays showed that leonurine significantly inhibited lipid accumulation and promoted ³H-cholesterol efflux in human THP-1 macrophage-derived foam cells in a concentration-dependent manner. Besides, both the mRNA and protein levels of ABCA1/G1, PPARγ and LXRα were enhanced by leonurine, which were attenuated by LXRα siRNA or PPARγ siRNA transfection. Finally, leonurine improved plasma lipid profile, decreased atherosclerotic lesion sizes, increased collagen contents and amplified PPARγ, LXRα and ABCA1/G1 expressions in aortic roots of apoE^-/- mice. Conclusions: Leonurine can promote cholesterol efflux and alleviate cellular lipid accumulation by magnifying the expression of ABCA1/G1 in a PPARγ/LXRα signaling pathway-dependent manner in human THP-1 macrophage-derived foam cells and abate atherogenesis in apoE^-/- mice, which may offer a promising therapeutic intervention of leonurine in protecting against AS.

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Section: Methodsmentioning

confidence: 99%

Leonurine Prevents Atherosclerosis Via Promoting the Expression of ABCA1 and ABCG1 in a Pparγ/Lxrα Signaling Pathway-Dependent Manner

Jiang

Ren

Chen

et al. 2017

Cell Physiol Biochem

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“…El 13% de la variación en la expresión del miR-155 se debe a un efecto positivo de la testosterona libre sérica. Estos miRNAs influencian la expresión de 73 genes que están involucrados en el metabolismo de lípidos, carbohidratos, secreción y acción hormonal, procesos inflamatorio y desarrollo del sistema endocrino [37][38][39][40] .…”

Section: Micrornas En Sopunclassified

Epigenética del síndrome de ovario poliquístico

Recabarren³

et al. 2017

Rev. méd. Chile

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Epigenetics of polycystic ovary syndromeE l síndrome de ovario poliquístico (SOP), es una disfunción endocrino-metabólica con una prevalencia de 6-10% en mujeres de edad reproductiva 1,2 . Se caracteriza por hiperandrogenismo, oligo-anovulación y morfología de ovario poliquística. Representa la causa más frecuente de infertilidad anovulatoria e hiperandrogenismo en mujeres. Además, la mayoría de las pacientes con SOP desarrollan resistencia insulínica periférica (RI) y poseen un mayor riesgo de desarrollar diabetes mellitus tipo 2 (DM2) 3 .Su etiología es multifactorial, y es tema de investigación relevante ya que el SOP se considera un desorden genético complejo, en donde numerosos genes contribuyen parcialmente al fenotipo sin que alguno de ellos constituya uno de riesgo mayor. El fenotipo es también influenciado por factores ambientales 4,5 , siendo finalmente la suma de ambos factores, la que gatilla el síndrome [6][7][8] . A esto debemos sumar la acción del ambiente, siendo finalmente la suma de ambos factores, los gatilladores del síndrome 7,8 . Se ha sugerido que existen factores genéticos implicados en el desarrollo del síndrome, debido a que se ha observado un fuerte componente de agregación familiar, siendo afectados entre 20-40% de los parientes de primer grado de las mujeres con SOP 9-12 . En los últimos años se ha propuesto que la programación fetal podría estar implicada en la patogenia del SOP. En este contexto la exposición prenatal a andrógenos (EPA) podría ser uno de los principales candidatos como factor reprogramador [13][14][15][16][17][18][19][20] . Independiente de la fuente del exceso de andrógenos prenatal, estas observaciones sugieren que el SOP podría ser programado in utero por la influencia del aumento de andrógenos e insulina, posiblemente mediante la alteración de la expresión génica durante la vida pre y post-natal.Esta revisión tiene por objetivo analizar la información disponible hasta el día de hoy relacionada a dos mecanismos de regulación epigenética como son las metilaciones del ADN y el papel de

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“…МиРНК-27a может принимать участие практически во всех известных процессах, способствующих или непосредственно приводящих к развитию и прогрессированию атеросклерза, включая воспаление, обмен липидов, окислительный стресс, инсулинорезистентность, гипергликемию [14].…”

Section: результаты и обсуждениеunclassified

Profile of microRNAs associated with coronary heart disease in patients with type 2 diabetes

et al. 2016

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Introduction. Cardiovascular disease (CVD) remain the leading cause of death in industrialized countries. Patients with coronary heart disease (CHD) in combination with diabetes mellitus type 2 (T2DM) are characterized by more severe CHD and poor prognosis. Resent data indicate microRNAs (miRNAs) as important participants in the pathogenesis of various pathological conditions, including obesity, T2DM and CVD.The aim of this study was to determine expression of miRNAs associated with the development of CHD, and transforming growth factor beta (TGF-β) in a patients with T2DM and obesity Materials and methods. 42 patients with 1-2 degrees obesity and diagnosed T2DM were divided into 2 groups. The first group with CHD, the second group - without CHD. 9 miRNAs were evaluated: miRNA-1, miRNA-21, miRNA-26a, m miRNA-27, miRNA-33a, miRNA-33b, miRNA-133a, miRNA-133b, miRNA-208.Results and discussion. Significant differences were found in expression of miRNA-21, miRNA-26a, miRNA27a. An increased expression of miRNA-21, miRNA-27a was found in patients CHD while the expression of miRNA-26a was reduced in comparison with the group without CHD.Conclusion. The results of this study may be an initial step for the detection of molecular basis in CHD pathogenesis in these patients by quantifying miRNA expression. Introduction. Cardiovascular disease (CVD) remain the leading cause of death in industrialized countries. Patients with coronary heartdisease (CHD) in combination with diabetes mellitus type 2 (T2DM) are characterized by more severe CHD and poor prognosis. Resent data indicate microRNAs (miRNAs) as important participants in the pathogenesis of various pathological conditions, including obesity, T2DM and CVD.The aim of this study was to determine expression of miRNAs associated with the development of CHD, and transforming growth factor beta (TGF-β) in a patients with T2DM and obesity Materials and methods. 42 patients with 1-2 degrees obesity and diagnosed T2DM were divided into 2 groups. The first group with CHD, the second group - without CHD. 9 miRNAs were evaluated: miRNA-1, miRNA-21, miRNA-26a, m miRNA-27, miRNA-33a, miRNA-33b, miRNA-133a, miRNA-133b, miRNA-208.Results and discussion. Significant differences were found in expression of miRNA-21, miRNA-26a, miRNA27a. An increased expression of miRNA-21, miRNA-27a was found in patients CHD while the expression of miRNA-26a was reduced in comparison with the group without CHD.Conclusion. The results of this study may be an initial step for the detection of molecular basis in CHD pathogenesis in these patients by quantifying miRNA expression.

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The magic and mystery of MicroRNA-27 in atherosclerosis

Cited by 110 publications

References 66 publications

Leonurine Prevents Atherosclerosis Via Promoting the Expression of ABCA1 and ABCG1 in a Pparγ/Lxrα Signaling Pathway-Dependent Manner

Leonurine Prevents Atherosclerosis Via Promoting the Expression of ABCA1 and ABCG1 in a Pparγ/Lxrα Signaling Pathway-Dependent Manner

Epigenética del síndrome de ovario poliquístico

Profile of microRNAs associated with coronary heart disease in patients with type 2 diabetes

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