The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

Freisleben, Hans-Joachim

doi:10.3390/ijms20205217

Cited by 10 publications

(7 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This indicates an excellent cytotoxic efficacy and an improved nebulisation suitability of liposomal formulations containing tetraether lipids due to the strong adhesion forces within their lipid bilayer [ 62 ]. Besides, the ability of TEL to positively affect the intermembrane exchange of the active ingredient, thus enhancing the delivery [ 64 ], was also confirmed by these results. The difference between dark control and irradiated lyophilised and nebulised curcumin-liposomes was also significant ( p < 0.05) for DT and DC, but not for DD.…”

Section: Resultsmentioning

confidence: 70%

“…Both, EE and LC seemed quite stable after the process of nebulisation, which speaks again for the high degree of membrane stability as it makes the liposomes less permeable by preserving membrane integrity. Stabilisation through the bipolar structure of TEL also provides thermal stability [ 63 ] and better long-term storage stability rates [ 64 ]. Besides, TEL can positively affect the intermembrane exchange of the active ingredient and thereby enhance the delivery [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

“…Stabilisation through the bipolar structure of TEL also provides thermal stability [ 63 ] and better long-term storage stability rates [ 64 ]. Besides, TEL can positively affect the intermembrane exchange of the active ingredient and thereby enhance the delivery [ 64 ]. The linkages of Cholesterol inside the lipid bilayer of DC similarly promote the encapsulation of curcumin [ 60 ], but with 88.4% ( Table 3 ) to a lower extent.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

et al. 2021

View full text Add to dashboard Cite

Lung cancer is one of the most common causes for a high number of cancer related mortalities worldwide. Therefore, it is important to improve the therapy by finding new targets and developing convenient therapies. One of these novel non-invasive strategies is the combination of pulmonary delivered tetraether liposomes and photodynamic therapy. In this study, liposomal model formulations containing the photosensitiser curcumin were nebulised via two different technologies, vibrating-mesh nebulisation and air-jet nebulisation, and compared with each other. Particle size and ζ-potential of the liposomes were investigated using dynamic light scattering and laser Doppler anemometry, respectively. Furthermore, atomic force microscopy and transmission electron microscopy were used to determine the morphological characteristics. Using a twin glass impinger, suitable aerodynamic properties were observed, with the fine particle fraction of the aerosols being ≤62.7 ± 1.6%. In vitro irradiation experiments on lung carcinoma cells (A549) revealed an excellent cytotoxic response of the nebulised liposomes in which the stabilisation of the lipid bilayer was the determining factor. Internalisation of nebulised curcumin-loaded liposomes was visualised utilising confocal laser scanning microscopy. Based on these results, the pulmonary application of curcumin-loaded tetraether liposomes can be considered as a promising approach for the photodynamic therapy against lung cancer.

show abstract

Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

et al. 2021

View full text Add to dashboard Cite

show abstract

“…However, instability of the BLM made of conventional diester lipids has been a major drawback in these applications [ 5 ]. To remedy this problem, tough materials such as archaea tetraether lipids (reviewed in [ 6 ]), triblock copolymers [ 7 , 8 ] and polymerized lipids [ 3 ] have been used. Since the structure of lipids can affect biological activities of membrane-bound proteins [ 9 ], it is important to note that triblock copolymers and polymerized lipids are structurally very different from naturally occurring lipids, which could lead to unnatural responses from a biological sample.…”

Section: Introductionmentioning

confidence: 99%

Certain, but Not All, Tetraether Lipids from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius Can Form Black Lipid Membranes with Remarkable Stability and Exhibiting Mthk Channel Activity with Unusually High Ca2+ Sensitivity

Bonanno

Chong

2021

IJMS

View full text Add to dashboard Cite

Bipolar tetraether lipids (BTL) have been long thought to play a critical role in allowing thermoacidophiles to thrive under extreme conditions. In the present study, we demonstrated that not all BTLs from the thermoacidophilic archaeon Sulfolobus acidocaldarius exhibit the same membrane behaviors. We found that free-standing planar membranes (i.e., black lipid membranes, BLM) made of the polar lipid fraction E (PLFE) isolated from S. acidocaldarius formed over a pinhole on a cellulose acetate partition in a dual-chamber Teflon device exhibited remarkable stability showing a virtually constant capacitance (~28 pF) for at least 11 days. PLFE contains exclusively tetraethers. The dominating hydrophobic core of PLFE lipids is glycerol dialky calditol tetraether (GDNT, ~90%), whereas glycerol dialkyl glycerol tetraether (GDGT) is a minor component (~10%). In sharp contrast, BLM made of BTL extracted from microvesicles (Sa-MVs) released from the same cells exhibited a capacitance between 36 and 39 pF lasting for only 8 h before membrane dielectric breakdown. Lipids in Sa-MVs are also exclusively tetraethers; however, the dominating lipid species in Sa-MVs is GDGT (>99%), not GDNT. The remarkable stability of BLMPLFE can be attributed to strong PLFE–PLFE and PLFE–substrate interactions. In addition, we compare voltage-dependent channel activity of calcium-gated potassium channels (MthK) in BLMPLFE to values recorded in BLMSa-MV. MthK is an ion channel isolated from a methanogenic that has been extensively characterized in diester lipid membranes and has been used as a model for calcium-gated potassium channels. We found that MthK can insert into BLMPLFE and exhibit channel activity, but not in BLMSa-MV. Additionally, the opening/closing of the MthK in BLMPLFE is detectable at calcium concentrations as low as 0.1 mM; conversely, in diester lipid membranes at such a low calcium concentration, no MthK channel activity is detectable. The differential effect of membrane stability and MthK channel activity between BLMPLFE and BLMSa-MV may be attributed to their lipid structural differences and thus their abilities to interact with the substrate and membrane protein. Since Sa-MVs that bud off from the plasma membrane are exclusively tetraether lipids but do not contain the main tetraether lipid component GDNT of the plasma membrane, domain segregation must occur in S. acidocaldarius. The implication of this study is that lipid domain formation is existent and functionally essential in all kinds of cells, but domain formation may be even more prevalent and pronounced in hyperthermophiles, as strong domain formation with distinct membrane behaviors is necessary to counteract randomization due to high growth temperatures while BTL in general make archaea cell membranes stable in high temperature and low pH environments whereas different BTL domains play different functional roles.

show abstract

“…Studies of the organization of archaeal lipids in artificial membranes between two water environments indicate that in these systems lipids are generally organised as monolayers of fully expanded molecules, normal to the membrane fields [8]. Archaeal tetraether lipids can be of great value in bioelectronics, biomedicine and nanotechnology, because they can self-assemble into an ordered structure with tremendous stability against chemical degradation and microbes [9] [10] [11].…”

Section: Introductionmentioning

confidence: 99%

Stability Behaviour of Monolayer Tetraether Lipids on the Amino-Silanised Silicon Wafer: Comparative Study between Langmuir-Blodgett Monolayers with Self-Assembled Monolayers

Vidawati¹,

Bakowsky²,

Rothe³

2020

AMPC

View full text Add to dashboard Cite

This study investigated the stability behaviour of molecular monolayer symmetric chemically modified tetraether lipids caldarchaeol-PO 4 on the amino-silanised silicon wafer using Langmuir-Blodgett films, Self Assembling Monolayers (SAMs), ellipsometry, and atomic force microscopy (AFM). The monolayers of caldarchaeol-PO 4 were stable on the solid surface amino-silanised silicon wafer. The organizations of molecular monolayers caldarchaeol-PO 4 by Langmuir-Blodgett method and SAMs have been analyzed. The surface of pressure in Langmuir-Blodgett processing is carried out monolayers caldarchaeol-PO 4 more flat island inhomogeneous. Another method of monolayers caldarchaeol-PO 4 by SAMs is showed a large flat domain. Monolayers caldarchaeol-PO 4 by Langmuir-Blodgett method seems to be stable and chemically resistant after washing with organic solvent and an additional treatment ultrasonification with various thickness lipids arround 2 nm to 6 nm. Conversely, monolayer caldarchaeol-PO 4 by SAMs appears fewer than monolayers caldarchaeol-PO 4 by Langmuir-Blodgett method, the thickness of various from 1 nm to 3 nm.

show abstract

The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

Cited by 10 publications

References 69 publications

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

Improvement of Pulmonary Photodynamic Therapy: Nebulisation of Curcumin-Loaded Tetraether Liposomes

Certain, but Not All, Tetraether Lipids from the Thermoacidophilic Archaeon Sulfolobus acidocaldarius Can Form Black Lipid Membranes with Remarkable Stability and Exhibiting Mthk Channel Activity with Unusually High Ca2+ Sensitivity

Stability Behaviour of Monolayer Tetraether Lipids on the Amino-Silanised Silicon Wafer: Comparative Study between Langmuir-Blodgett Monolayers with Self-Assembled Monolayers

Contact Info

Product

Resources

About