The Maintenance of Human Normal Cells and Tumor Cells in Continuous Culture: I. Preliminary Report: Cultivation of Mesoblastic Tumors and Normal Tissue and Notes on Methods of Cultivation

Gey, George O.; Gey, Margaret K.

doi:10.1158/ajc.1936.45

Cited by 629 publications

(221 citation statements)

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“…Islets of Langerhans were isolated from rat pancreas by collagenase digestion [11] and incubated for 60 min at 37°C in a bicarbonate-buffered (pH 7.4) physiological salt solution [12] containing 2 mM glucose, 2 mM CaCI2 and 0.5 mg/ml bovine serum albumin (BSA, fraction V). In experiments using intact islets, groups of three islets were incubated for 60 min at 37°C in 0.6 ml of the physiological salt solution containing the test substances of interest.…”

Section: Methodsmentioning

confidence: 99%

Catecholamine inhibition of Ca²⁺‐induced insulin secretion from electrically permeabilised islets of Langerhans

et al. 1987

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Noradrenaline (1-10 gM) inhibited Ca 2 +-induced insulin secretion from electrically permeabilised islets of Langerhans with an efficacy similar to that for inhibition of glucose-induced insulin secretion from intact islets. The inhibition of insulin secretion from permeabilised islets was blocked by the ~-adrenoreceptor antagonist, yohimbine. Adenosine Y,5'-cyclic monophosphate (cAMP) did not relieve the noradrenaline inhibition of Ca 2 +-induced secretion from the permeabilised islets, although noradrenaline did not affect the secretory responses to cAMP at substimulatory (50 nM) concentrations of Ca 2 +. These results suggest that catecholamines do not inhibit insulin secretion solely by reducing B-cell adenylate cyclase activity, and imply that one site of action of noradrenaline is at a late stage in the secretory process.

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Section: Methodsmentioning

confidence: 99%

Catecholamine inhibition of Ca²⁺‐induced insulin secretion from electrically permeabilised islets of Langerhans

et al. 1987

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“…Islets of Langerhans were isolated from rat pancreas by collagenase digestion HZ] and incubated for 60 min at 37Y.Z in a bicarbonate-buffered physiological salt solution [13] containing 2 mM glucose, 2 mM GaCl2 and 0.5 mg/ml bovine serum albumen (BSA, fraction V, Sigma}. Isolated islets were permeabilised by exposure to a high-intensity electric field, as described [ Differences between means were assessed by analysis of variance or Student's unpaired t-test, as appropriate.…”

Section: Methodsmentioning

confidence: 99%

Regulation of insulin secretion by cAMP in rat islets of Langerhans permeabilised by high‐voltage discharge

1986

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Adenosine 3',5-cyclic monophosphate (CAMP) was shown to stimulate ins&in secretion from electrically permeabilised islets of Langerhans incubated in Ca2+/EGTA buffers. CAMP-induced insulin secretion occurred in the presence of either sub-stimulatory (50 nM) or stimulatory (> 100 nM) concentrations of Ca2+.Similar effects on secretion were obtained in response to I-bromo-CAMP (8-Br-CAMP) or the phosphodiesterase inhibitor, 3-isobutyl-l-methylxanthine. Forskolin (0.2-20 PM) increased adenylate cyclase activity and enhanced insulin secretion from the ~~eabili~ islets. These results suggest that, in electrically permeabilised islets, CAMP-indu~d insulin secretion is not dependent on changes in cytosohc Ca2+. Insulin secretion (Islets of Langerhans)Electrical permeabilization cyclic AMP Forskolin Isobutylmethylxanthine

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“…The isolation medium was a bicarbonate buffered physiological saline solution equilibrated to pH 7.4 by gassing with 02:CO2 (95:5) (Gey & Gey, 1936). The buffer was supplemented with 4 mM glucose and 1 mM CaCl2.…”

Section: Isolation Of Islets Of Langerhansmentioning

confidence: 99%

Antagonism of the stimulatory effects of efaroxan and glibenclamide in rat pancreatic islets by the imidazoline, RX801080

Brown

Chan

Stillings³

et al. 1993

British J Pharmacology

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1 The imidazoline M2-adrenoceptor antagonist, efaroxan, stimulates insulin secretion from rat isolated islets and antagonizes the ability of diazoxide to inhibit glucose-induced insulin secretion. These effects result from closure of ATP-sensitive potassium channels although the mechanisms involved have not been elucidated. 2 In the present work, we have examined the effects of a close structural analogue of efaroxan, RX801080, in rat isolated islets of Langerhans. RX801080 was found to be ineffective as a stimulator of insulin secretion and did not prevent the inhibition of insulin secretion mediated by diazoxide. 3 RX801080 acted as an antagonist of the actions of several imidazolines (efaroxan, phentolamine and midaglizole) in rat islets. It dose-dependently inhibited the ability of efaroxan to antagonize the effects of diazoxide in islets and also completely inhibited the direct stimulation of insulin secretion mediated by efaroxan. 4 RX801080 also antagonized the effects of the non-imidazoline, ATP-sensitive potassium channel blocker, glibenclamide, in rat islets. It inhibited both the capacity of glibenclamide to stimulate insulin secretion and the ability of glibenclamide to overcome the inhibitory effects of diazoxide in rat islets. 5 Antagonism of glibenclamide responses by RX801080 was not due to inhibition of binding of the sulphonylurea to its receptor on the pancreatic P-cell. 6 The results suggest that imidazoline compounds and sulphonylureas interact with distinct binding sites on islet cells, but that these sites can interact functionally to control islet cell ATP-sensitive potassium channel activity and insulin secretion.

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The Maintenance of Human Normal Cells and Tumor Cells in Continuous Culture: I. Preliminary Report: Cultivation of Mesoblastic Tumors and Normal Tissue and Notes on Methods of Cultivation

Cited by 629 publications

References 4 publications

Catecholamine inhibition of Ca²⁺‐induced insulin secretion from electrically permeabilised islets of Langerhans

Catecholamine inhibition of Ca²⁺‐induced insulin secretion from electrically permeabilised islets of Langerhans

Regulation of insulin secretion by cAMP in rat islets of Langerhans permeabilised by high‐voltage discharge

Antagonism of the stimulatory effects of efaroxan and glibenclamide in rat pancreatic islets by the imidazoline, RX801080

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The Maintenance of Human Normal Cells and Tumor Cells in Continuous Culture: I. Preliminary Report: Cultivation of Mesoblastic Tumors and Normal Tissue and Notes on Methods of Cultivation

Cited by 629 publications

References 4 publications

Catecholamine inhibition of Ca2+‐induced insulin secretion from electrically permeabilised islets of Langerhans

Catecholamine inhibition of Ca2+‐induced insulin secretion from electrically permeabilised islets of Langerhans

Regulation of insulin secretion by cAMP in rat islets of Langerhans permeabilised by high‐voltage discharge

Antagonism of the stimulatory effects of efaroxan and glibenclamide in rat pancreatic islets by the imidazoline, RX801080

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Catecholamine inhibition of Ca²⁺‐induced insulin secretion from electrically permeabilised islets of Langerhans

Catecholamine inhibition of Ca²⁺‐induced insulin secretion from electrically permeabilised islets of Langerhans