2023
DOI: 10.1101/2023.02.06.527228
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The maize preligule band is subdivided into distinct domains with contrasting cellular properties prior to ligule outgrowth

Abstract: The maize ligule is a fringe of epidermis-derived tissue, which arises from the preligule band (PLB) at a boundary between the blade and sheath. A hinge-like auricle also develops immediately distal to the ligule and contributes to blade angle. Here, we characterize the stages of PLB and early ligule development in terms of topography, cell area, division orientation, cell wall rigidity, and auxin response dynamics. Differential thickening of epidermal cells and localized periclinal divisions contributed to th… Show more

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“…Three to five-week-old maize plants were used and leaves were removed until the ligule height was < 2 mm, and abaxial symmetrically dividing leaf epidermal samples were imaged. While imaging PIN1-YFP, the developing ligule was selected for imaging, as described (Neher et al, 2023). Samples were mounted in water.…”
Section: Methodsmentioning
confidence: 99%
“…Three to five-week-old maize plants were used and leaves were removed until the ligule height was < 2 mm, and abaxial symmetrically dividing leaf epidermal samples were imaged. While imaging PIN1-YFP, the developing ligule was selected for imaging, as described (Neher et al, 2023). Samples were mounted in water.…”
Section: Methodsmentioning
confidence: 99%