The maize ZmMYB42 represses the phenylpropanoid pathway and affects the cell wall structure, composition and degradability in Arabidopsis thaliana

Sonbol, F.M.; Fornalé, Silvia; Capellades, Montserrat; Encina, Antonio; Touriño, Sonia; Torres, Josep Lluı́s; Rovira, Pere; Ruel, K.; Puigdomènech, Pere; Rigau, Joan; Caparrós-Ruiz, David

doi:10.1007/s11103-009-9473-2

Cited by 153 publications

(146 citation statements)

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“…MYB42 was able to interact in vivo with the cesa7 promoter (Supplemental Figure 8). This is in line with previous results showing that MYB42 alters cell wall polysaccharides biosynthesis when expressed in Arabidopsis (Sonbol et al, 2009). …”

Section: The Lignin Repressors Myb31 and Myb42 Interact With Zml2 Andsupporting

confidence: 93%

“…As previously mentioned, MYB11 is evolutionarily closely related to MYB31 and MYB42, which are already described as lignin repressors (Fornalé et al, 2006(Fornalé et al, , 2010Sonbol et al, 2009). We performed ChIP-qPCR using specific antibodies against MYB31 and MYB42 and determined that they bind in vivo to 7 and 5 lignin genes, respectively (Figure 7; Supplemental Figure 8).…”

Section: The Lignin Repressors Myb31 and Myb42 Interact With Zml2 Andmentioning

confidence: 70%

“…Several studies have identified different regulators of lignin biosynthesis in grasses (reviewed in Gray et al, 2012), such as maize (Zea mays) MYB31 and MYB42, two subgroup 4 R2R3-MYB transcription factors that act as repressors of the lignin pathway (Fornalé et al, 2006(Fornalé et al, , 2010Sonbol et al, 2009). Despite their different roles in lignin regulation, both factors repress the maize caffeic acid O-methyl transferase (comt) gene (AC196475).…”

Section: Introductionmentioning

confidence: 99%

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A MYB/ZML Complex Regulates Wound-Induced Lignin Genes in Maize

et al. 2015

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Lignin is an essential polymer in vascular plants that plays key structural roles in vessels and fibers. Lignification is induced by external inputs such as wounding, but the molecular mechanisms that link this stress to lignification remain largely unknown. In this work, we provide evidence that three maize (Zea mays) lignin repressors, MYB11, MYB31, and MYB42, participate in wound-induced lignification by interacting with ZML2, a protein belonging to the TIFY family. We determined that the three R2R3-MYB factors and ZML2 bind in vivo to AC-rich and GAT(A/C) cis-elements, respectively, present in a set of lignin genes. In particular, we show that MYB11 and ZML2 bind simultaneously to the AC-rich and GAT(A/C) cis-elements present in the promoter of the caffeic acid O-methyl transferase (comt) gene. We show that, like the R2R3-MYB factors, ZML2 also acts as a transcriptional repressor. We found that upon wounding and methyl jasmonate treatments, MYB11 and ZML2 proteins are degraded and comt transcription is induced. Based on these results, we propose a molecular regulatory mechanism involving a MYB/ZML complex in which wound-induced lignification can be achieved by the derepression of a set of lignin genes.

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Section: The Lignin Repressors Myb31 and Myb42 Interact With Zml2 Andsupporting

confidence: 93%

Section: The Lignin Repressors Myb31 and Myb42 Interact With Zml2 Andmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

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A MYB/ZML Complex Regulates Wound-Induced Lignin Genes in Maize

et al. 2015

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“…9,12,16,21 In addition, some MYBs have been suggested to function as repressors in fine-tuning the expression level of phenylpropanoid biosynthetic genes. [46][47][48][49][50] Identification and characterization of all the cis elements and transcription factors involved in regulation of lignin biosynthesis will be essential for gaining a full picture of the complexity of transcriptional control of lignin biosynthesis. Second, little is known about how lignin heterogeneity is regulated at the transcription level.…”

Section: Acknowledgementsmentioning

confidence: 99%

Transcriptional regulation of lignin biosynthesis

Zhong

Ye²

2009

Plant Signaling & Behavior

224

162

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“…The MYB4 homologs from Arabidopsis and petunia directly repress phenylpropanoid biosynthetic genes, such as CINNAMATE-4-HYDROXYLASE (C4H) or 4-COUMARATE CoA LIGASE (4CL), responsible for the synthesis of UV-protecting sinapate esters or the floral volatiles phenylacetaldehyde and methyl benzoate (Jin et al, 2000;Colquhoun et al, 2011). In maize, ZmMYB42 and ZmMYB31 repress lignin genes affecting cell wall structure, composition, and degradability (Sonbol et al, 2009;Fornalé et al, 2010). MYB repressors specifically regulating flavonoid synthesis have also been found.…”

mentioning

confidence: 99%

The Phenylpropanoid Pathway Is Controlled at Different Branches by a Set of R2R3-MYB C2 Repressors in Grapevine

Cavallini

Matus²,

Finezzo³

et al. 2015

Plant Physiol.

274

280

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Because of the vast range of functions that phenylpropanoids possess, their synthesis requires precise spatiotemporal coordination throughout plant development and in response to the environment. The accumulation of these secondary metabolites is transcriptionally controlled by positive and negative regulators from the MYB and basic helix-loop-helix protein families. We characterized four grapevine (Vitis vinifera) R2R3-MYB proteins from the C2 repressor motif clade, all of which harbor the ethylene response factor-associated amphiphilic repression domain but differ in the presence of an additional TLLLFR repression motif found in the strong flavonoid repressor Arabidopsis (Arabidopsis thaliana) AtMYBL2. Constitutive expression of VvMYB4a and VvMYB4b in petunia (Petunia hybrida) repressed general phenylpropanoid biosynthetic genes and selectively reduced the amount of small-weight phenolic compounds. Conversely, transgenic petunia lines expressing VvMYBC2-L1 and VvMYBC2-L3 showed a severe reduction in petal anthocyanins and seed proanthocyanidins together with a higher pH of crude petal extracts. The distinct function of these regulators was further confirmed by transient expression in tobacco (Nicotiana benthamiana) leaves and grapevine plantlets. Finally, VvMYBC2-L3 was ectopically expressed in grapevine hairy roots, showing a reduction in proanthocyanidin content together with the down-regulation of structural and regulatory genes of the flavonoid pathway as revealed by a transcriptomic analysis. The physiological role of these repressors was inferred by combining the results of the functional analyses and their expression patterns in grapevine during development and in response to ultraviolet B radiation. Our results indicate that VvMYB4a and VvMYB4b may play a key role in negatively regulating the synthesis of small-weight phenolic compounds, whereas VvMYBC2-L1 and VvMYBC2-L3 may additionally fine tune flavonoid levels, balancing the inductive effects of transcriptional activators.

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The maize ZmMYB42 represses the phenylpropanoid pathway and affects the cell wall structure, composition and degradability in Arabidopsis thaliana

Cited by 153 publications

References 70 publications

A MYB/ZML Complex Regulates Wound-Induced Lignin Genes in Maize

A MYB/ZML Complex Regulates Wound-Induced Lignin Genes in Maize

Transcriptional regulation of lignin biosynthesis

The Phenylpropanoid Pathway Is Controlled at Different Branches by a Set of R2R3-MYB C2 Repressors in Grapevine

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