The major Alternaria alternata allergen, Alt a 1: A reliable and specific marker of fungal contamination in citrus fruits

Gabriel, Marta Fonseca; N, Uriel; Teifoori, Fardis; Postigo, Idoia; Suñén, Ester; Martı́nez, Jorge

doi:10.1016/j.ijfoodmicro.2017.06.006

Cited by 35 publications

(35 citation statements)

References 22 publications

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“…In recent years, several fungal allergens (Alt a 1, Alt a 6, Asp f 1, Asp f 3, Asp n 1, Asp n 3, and others) have been presented as valuable molecular markers of allergenicity and pathogenicity (Matricardi et al, 2016). Accordingly, it is expected that the use of approaches targeting the Asp n 3 gene and/or protein to unequivocally identify and detect the pathogen A. niger will yield privileged and powerful information regarding the quality and biosecurity control of foodstuffs (Gabriel et al, 2016b(Gabriel et al, , 2017.…”

Section: Discussionmentioning

confidence: 99%

“…Aspergillus niger was grown on malt extract agar plates for 4 days at 25 • C. The spores were collected in 0.2% agar and 0.05% Tween 80 solution. Then, the spore suspension was diluted 1:100, counted in a Neubauer chamber, and adjusted to a final concentration of 1 × 10 6 spores/ml with sterile phosphatebuffered saline (PBS) and 0.05 Tween 80 (Gabriel et al, 2017).…”

Section: Fungal Strains and Culture Conditionsmentioning

confidence: 99%

“…The traditional identification of fungi by means of methodologies based on morphological characteristics has a low degree of sensitivity and requires considerable expertise. To address these shortcomings, in recent years, molecular methods such as polymerase chain reaction (PCR) have been considered suitable alternatives for the rapid and early diagnosis of fungal contamination (Gabriel et al, 2015(Gabriel et al, , 2017.…”

Section: Introductionmentioning

confidence: 99%

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The Aspergillus niger Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in Mangifera indica Fruit

et al. 2021

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The aim of this work was to study the value of the main allergen Asp n 3 of Aspergillus niger as a molecular marker of allergenicity and pathogenicity with the potential to be used in the identification of A. niger as a contaminant and cause of spoilage of Mangifera indica. Real-time polymerase chain reaction (RT-PCR) was used for the amplification of Asp n 3 gene. Two pairs of primers were designed: one for the amplification of the entire sequence and another one for the amplification of the most conserved region of this peroxisomal protein. The presence of A. niger was demonstrated by the early detection of the allergenic protein Asp n 3 coding gene, which could be considered a species-specific marker. The use of primers designed based on the conserved region of the Asp n 3 encoding gene allowed us to identify the presence of the closely related fungal species Aspergillus fumigatus by detecting Asp n 3 homologous protein, which can be cross-reactive. The use of conserved segments of the Asp n 3 gene or its entire sequence allows us to detect phylogenetically closely related species within the Aspergilaceae family or to identify species-specific contaminating fungi.

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Section: Discussionmentioning

confidence: 99%

Section: Fungal Strains and Culture Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Aspergillus niger Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in Mangifera indica Fruit

et al. 2021

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“…Genes such as histone‐3, glyceraldehyde 3‐phosphate dehydrogenase ( Gpd ), Alt a1 , AaSdhB , AaSdhC , AaSdhD, ITS and β‐tubulin have been used for Alternaria species identification (Table 2). The Alt a1 gene is a widely used marker for Alternaria species (Gabriel et al , 2017). The polyketide synthetase (PKS) gene and nonribosomal peptide synthesis (NRPS) gene are essential for Alternaria toxin synthesis and regulation and can also be used to identify Alternaria toxin‐producing species (Chen et al , 2015).…”

Section: Wgs and Genetic Marker Identification Of Related Microbes Onmentioning

confidence: 99%

DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables

Shen

Nie

Kuang

et al. 2020

Microbial Biotechnology

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Summary The development of DNA sequencing technology has provided an effective method for studying foodborne and phytopathogenic microorganisms on fruits and vegetables (F & V). DNA sequencing has successfully proceeded through three generations, including the tens of operating platforms. These advances have significantly promoted microbial whole‐genome sequencing (WGS) and DNA polymorphism research. Based on genomic and regional polymorphisms, genetic markers have been widely obtained. These molecular markers are used as targets for PCR or chip analyses to detect microbes at the genetic level. Furthermore, metagenomic analyses conducted by sequencing the hypervariable regions of ribosomal DNA (rDNA) have revealed comprehensive microbial communities in various studies on F & V. This review highlights the basic principles of three generations of DNA sequencing, and summarizes the WGS studies of and available DNA markers for major bacterial foodborne pathogens and phytopathogenic fungi found on F & V. In addition, rDNA sequencing‐based bacterial and fungal metagenomics are summarized under three topics. These findings deepen the understanding of DNA sequencing and its application in studies of foodborne and phytopathogenic microbes and shed light on strategies for the monitoring of F & V microbes and quality control.

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“…When the protein content present in fruit (for example, kiwifruit) is low, DNA‐based methods can be used to detect minute contaminations from the fruit. PCR or Rt‐PCR detection methods have been used to detect the concentrations of allergens present in citrus fruits (Gabriel et al., ), peaches (Graziano, Gullì, & Marmiroli, ), and strawberries (Ishibashi, Yoshikawa, & Uno, ), and is highly specific and sensitive compared to the traditional detection methods (Holzhauser & Röder, ). As kiwifruit allergy is not as common as the “Big Eight” allergies (peanut, tree nut, milk, egg, soy, sesame, fish, and wheat allergies), there are very limited reports regarding the detection of kiwifruit or related commercial products.…”

Section: Pathogenesis Clinical Features Diagnosis and Epidemiologymentioning

confidence: 99%

A Comprehensive Review on Kiwifruit Allergy: Pathogenesis, Diagnosis, Management, and Potential Modification of Allergens Through Processing

Jin

Vanga

McCusker

et al. 2019

Comp Rev Food Sci Food Safe

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Kiwifruit is rich in bioactive components including dietary fibers, carbohydrates, natural sugars, vitamins, minerals, omega‐3 fatty acids, and antioxidants. These components are beneficial to boost the human immune system and prevent cancer and heart diseases. However, kiwifruit is emerging as one of the most common elicitors of food allergies worldwide. Kiwifruit allergy results from an abnormal immune response to kiwifruit proteins and occur after consuming this fruit. Symptoms range from the oral allergy syndrome (OAS) to the life‐threatening anaphylaxis. Thirteen different allergens have been identified in green kiwifruit and, among these allergens, Act d 1, Act d 2, Act d 8, Act d 11, and Act d 12 are defined as the “major allergens.” Act d 1 and Act d 2 are ripening‐related allergens and are found in abundance in fully ripe kiwifruit. Structures of several kiwifruit allergens may be altered under high temperatures or strong acidic conditions. This review discusses the pathogenesis, clinical features, and diagnosis of kiwifruit allergy and evaluates food processing methods including thermal, ultrasound, and chemical processing which may be used to reduce the allergenicity of kiwifruit. Management and medical treatments for kiwifruit allergy are also summarized.

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The major Alternaria alternata allergen, Alt a 1: A reliable and specific marker of fungal contamination in citrus fruits

Cited by 35 publications

References 22 publications

The Aspergillus niger Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in Mangifera indica Fruit

The Aspergillus niger Major Allergen (Asp n 3) DNA-Specific Sequence Is a Reliable Marker to Identify Early Fungal Contamination and Postharvest Damage in Mangifera indica Fruit

DNA sequencing, genomes and genetic markers of microbes on fruits and vegetables

A Comprehensive Review on Kiwifruit Allergy: Pathogenesis, Diagnosis, Management, and Potential Modification of Allergens Through Processing

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