The Major Protein of Chloroplast Stroma, Ribulosebisphosphate Carboxylase

Paech, Christian

doi:10.1007/978-3-642-82587-3_11

Cited by 3 publications

(2 citation statements)

References 201 publications

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“…The treatment of leaf segments with kinetin changed the polypeptides synthesized by chloroplasts (lane 2). Figure 4 shows, as Figure 1, that no polypeptide of 54 kDa, equivalent to the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) (Paech 1985), was detected among the proteins synthesized by chloroplasts from senescent leaves.…”

Section: Protein Syntheses By Senescent Chloroplastsmentioning

confidence: 94%

Translational control of chloroplast protein synthesis during senescence of barley leaves

Martı́n

Sabater

1989

Physiologia Plantarum

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A light‐driven homologous system for the translation of chloroplast mRNA has been prepared from chloroplasts isolated from senescent barley leaves and treated with micrococcal nuclease. We have investigated the potential of this system for studies on translational specificity. Chloroplast mRNAs from senescent and kinetin‐treated leaves have been translated in the homologous system and the products have been compared with the polypeptides synthesized by intact chloroplasts isolated from senescent and kinetin‐treated leaves. Isolated intact chloroplasts from senescent barley leaves specifically synthesized polypeptides of: 80,66,58,50,48 and 35 kDa but did not synthesize the 54 kDa large subunit of ribulose‐1, 5‐bisphosphate carboxylase oxygenase. Chloroplasts isolated from leaves treated for 18 h with kinetin specifically synthesized polypeptides of: 85,75 and 54 kDa. Several polypeptides (40,32,25 and 18 kDa) are synthesized by chloroplasts of both senescent and kinetin‐treated leaves. The homologous translation system derived from senescent chloroplasts synthesized only senescence polypeptides whether it was supplied with chloroplast mRNA from senescent leaves or leaves treated with kinetin for 18 h. The homologous translation system derived from chloroplasts of kinetin‐treated leaves synthesized either senescence polypeptides or kinetin‐induced polypeptides depending on the source of chloroplast mRNA, senescent leaves or leaves treated with kinetin, respectively. The results indicate that kinetin controls chloroplast gene expression in senescent leaves at both the transcriptional and translational level.

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Section: Protein Syntheses By Senescent Chloroplastsmentioning

confidence: 94%

Translational control of chloroplast protein synthesis during senescence of barley leaves

Martı́n

Sabater

1989

Physiologia Plantarum

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“…A pesquisa das classes das auronas e chalconas foi realizada após a extração inicial por decocção seguida de filtragem com acetato de etila, logo após, ocorreu a adição de solução aquosa de hidróxido de sódio, considerando-se positivas as amostras de tons vermelho-alaranjados [16] .…”

Section: Prospecção Fitoquímicaunclassified

Análise fitoquímica, toxicidade, potencial antioxidante e atividade antibacteriana da Ceiba speciosa (A.St.-Hil.) Ravenna

Braga

Barcelos

Calazans

et al. 2019

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O presente estudo realizou a prospecção fitoquímica e avaliou a toxicidade aguda, o potencial antioxidante e a atividade antibacteriana das flores da Ceiba speciosa. A triagem dos metabólitos foi realizada por reações colorimétricas, a toxicidade foi avaliada in vitro por meio do teste de letalidade frente ao microcrustáceo Artemia salina, a atividade antioxidante pelo sequestro de radicais livres, utilizando o radical sintético 2,2-difenil-1-picrilhidrazila (DPPH) e a atividade antimicrobiana pela difusão em disco frente às bactérias Escherichia coli, Shigella spp. e Staphylococcus aureus. Os resultados indicaram a presença de flavonoides e saponinas, observou-se a ausência da toxicidade das amostras (DL50=1.067 ug/mL) e a positividade para a atividade antioxidante (EC50=56,7 mg/mL). Em relação à atividade antibacteriana, as soluções hidrometanólicas a 80% (80:20, v/v) apresentaram efeito nas quatro maiores concentrações (100 mg/mL, 50 mg/mL, 25 mg/mL, 12,5 mg/mL) para as cepas de E. coli e Shiguela spp., e para o S. aureus o mesmo ocorreu a partir de 25 mg/mL. Concluiu-se sobre a ausência de toxicidade, positividade para o efeito antioxidante e atividade antibacteriana para as soluções hidrometanólicas. Sugere-se a continuidade das investigações para exploração do potencial biológico das flores de Ceiba speciosa, que pode constituir-se em uma alternativa como recurso terapêutico.

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Oxidative stress causes rapid membrane translocation and in vivo degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase.

Mehta¹,

Fawcett²,

Porath³

et al. 1992

Journal of Biological Chemistry

178

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The Major Protein of Chloroplast Stroma, Ribulosebisphosphate Carboxylase

Cited by 3 publications

References 201 publications

Translational control of chloroplast protein synthesis during senescence of barley leaves

Translational control of chloroplast protein synthesis during senescence of barley leaves

Análise fitoquímica, toxicidade, potencial antioxidante e atividade antibacteriana da Ceiba speciosa (A.St.-Hil.) Ravenna

Oxidative stress causes rapid membrane translocation and in vivo degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase.

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