The major proteins of the Escherichia coli outer cell envelope membrane: Evidence for the structural gene of protein II

Henning, Ulf; Hindennach, Ingrid; Haller, I.

doi:10.1016/0014-5793(76)80168-8

Cited by 39 publications

(21 citation statements)

References 8 publications

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“…From P678-54, ompA mutants were prepared by plating organisms with excess phage K3 and isolating resistant colonies. All non-mucoid strains resistant to phage K3 map at the ompA locus (Manning et al, 1976;Henning et al, 1976) but the ompA strains used here were also resistant to phage TuII* and to colicin L and were devoid of OmpA protein. One ompA mutant of strain P678-54 was used to prepare plasmid-bearing strains.…”

Section: Methodsmentioning

confidence: 99%

Suppression by the ColV, I-K94 Plasmid of a Growth Lesion in ompA Mutants of Escherichia coli

Deeney¹,

Goodson²,

Rossouw³

et al. 1986

Microbiology

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Section: Methodsmentioning

confidence: 99%

Suppression by the ColV, I-K94 Plasmid of a Growth Lesion in ompA Mutants of Escherichia coli

Deeney¹,

Goodson²,

Rossouw³

et al. 1986

Microbiology

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“…However, mutants with defects in the polysaccharide core of the LPS are poor recipients for R64drdll and other Ilike plasmids (Havekes et d., 19776;Manning & Reeves, 1977). Several F-mutants have been described which have altered LPS, although the decrease in transfer may be due to changes in outer-membrane proteins and/or the interaction between these proteins and LPS (Achtman et af., 1978;Henning et al, 1976;Puspurs et af., 1983).…”

Section: A G E N C O a N D V L Clarkmentioning

confidence: 99%

“…E. coli Fmutants that are deficient in conjugation (Con-) (Achtman et al, 1978;Havekes & Hoekstra, 1976;Henning et d., 1976;Schweizer & Henning, 1977) cannot form stable mating aggregates in liquid, but can form mating aggregates stable enough to allow DNA transfer on solid surfaces. Henning et al (1976) have shown that the gene product of the ompA cistron is needed for cells to function efficiently as recipients with Fcarrying donor cells. The ompA gene product, OmpA protein, is believed to be necessary for stabilization of mating aggregates rather than acting as a receptor for the F pilus.…”

Section: Introductionmentioning

confidence: 99%

Role of Outer-membrane Proteins and Lipopolysaccharide in Conjugation between Neisseria gonorrhoeae and Neisseria cinerea

Genco

Clark²

1988

Microbiology

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Little is known concerning the mechanism involved in cell contact between the donor and recipient during conjugation in Neisseria gonorrhoeae. The formation of stable mating pairs during conjugation in Escherichia coli appears to require a specific protein as well as LPS in the outer membrane of the recipient cell. To attempt to identify the cell surface components necessary for conjugation in the neisseriae, we began a comparison of the outer membrane of Neisseria cinerea strains that can (Con+) and cannot (Con-) serve as recipients in conjugation with N. gonorrhoeae. There were no differences in outer-membrane protein profiles on SDS-polyacrylamide gel electrophoresis between Con+ and Con- strains that could be correlated with the ability to conjugate. However, whole outer membrane isolated from Con+ strains specifically inhibited conjugation while those from Con- strains did not. Proteolytic cleavage of outer-membrane proteins by trypsin, pronase or alpha-chymotrypsin abolished the inhibitory effect of Con+ outer membranes, suggesting that these outer membranes contained a protease-sensitive protein(s) involved in conjugation. Although periodate oxidation of Con+ outer-membrane carbohydrates did not abolish the inhibitory action of these membranes, purified LPS from both Con+ and Con- strains inhibited conjugation when added at low concentrations. These results suggest that conjugation requires the presence of a specific conjugal receptor that consists of both LPS and one or more outer-membrane proteins. Both Con+ and Con- strains contain the necessary LPS, but only Con+ strains contain the required protein(s).

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“…The strict receptor specificity of many bacteriophages has made them useful tools in the study of bacterial surface structures [ 1 ]. Recently, several potential OM protein phages have been described for E. call, and some of them used to select mutants of OM proteins [3][4][5]. Recently, several potential OM protein phages have been described for E. call, and some of them used to select mutants of OM proteins [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Salmonella bacteriophages that use outer membrane protein receptors

Siitonen

1977

FEMS Microbiology Letters

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The major proteins of the Escherichia coli outer cell envelope membrane: Evidence for the structural gene of protein II

Cited by 39 publications

References 8 publications

Suppression by the ColV, I-K94 Plasmid of a Growth Lesion in ompA Mutants of Escherichia coli

Suppression by the ColV, I-K94 Plasmid of a Growth Lesion in ompA Mutants of Escherichia coli

Role of Outer-membrane Proteins and Lipopolysaccharide in Conjugation between Neisseria gonorrhoeae and Neisseria cinerea

Salmonella bacteriophages that use outer membrane protein receptors

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